Characterization of the complete mitochondrial genome of the brazilian cownose ray Rhinoptera brasiliensis (Myliobatiformes, Rhinopteridae) in the western Atlantic and its phylogenetic implications

Palacios-Barreto, Paola; Mar‐Silva, Adán Fernando; Bayona‐Vásquez, Natalia J.; Adams, Douglas H.; Díaz‐Jaimes, Píndaro

doi:10.1007/s11033-023-08272-0

Cited by 4 publications

(1 citation statement)

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“…Research on mitochondrial genomes have provided comprehensive insights into molecular evolution patterns, population dynamics, and adaptive processes across a wide array of organisms [1][2][3][4][5][6]. Mitochondrial genomes have been pivotal in molecular evolution and global genetic barcoding initiatives for species identification due to its small size, high substitution rate [7], lack of recombination and large copy number [8,9].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial genome structure and composition in 70 fishes: a key resource for fisheries management in the South Atlantic

Alvarenga,

D’Elia,

Rocha

et al. 2024

BMC Genomics

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Background Phylogenetic gaps of public databases of reference sequences are a major obstacle for comparative genomics and management of marine resources, particularly in the Global South, where economically important fisheries and conservation flagship species often lack closely-related references. We applied target-enrichment to obtain complete mitochondrial genomes of marine ichthyofauna from the Brazilian coast selected based on economic significance, conservation status and lack of phylogenetically-close references. These included sardines (Dorosomatidae, Alosidae), mackerels (Scombridae) croakers (Sciaenidae), groupers (Epinephelidae) and snappers (Lutjanidae). Results Custom baits were designed to enrich mitochondrial DNA across a broad phylogenetic range of fishes. Sequencing generated approximately 100k reads per sample, which were assembled in a total of 70 complete mitochondrial genomes and include fifty-two new additions to GenBank, including five species with no previous mitochondrial data. Departures from the typical gene content and order occurred in only three taxa and mostly involved tRNA gene duplications. Start-codons for all genes, except Cytochrome C Oxidase subunit I (COI), were consistently ATG, whilst a wide range of stop-codons deviated from the prevailing TAA. Phylogenetic analysis confirmed assembly accuracy and revealed signs of cryptic diversification within the Mullus genus. Lineage delimitation methods using Sardinella aurita and S. brasiliensis mitochondrial genomes support a single Operational Taxonomic Unit. Conclusions Target enrichment was highly efficient, providing complete novel mitochondrial genomes with little sequencing effort. These sequences are deposited in public databases to enable subsequent studies in population genetics and adaptation of Latin American fish species and serve as a vital resource for conservation and management programs that rely on molecular data for species and genus-level identification.

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