Characterization of the complete mitochondrial genome sequence of the dog roundworm<i>Toxascaris leonina</i>(Nematoda, Ascarididae) from China

Xie, Yue; Li, Hongyu; Wang, Chengdong; Li, Yingxin; Liu, Yunjian; Meng, Xiaduo; Wang, Lu; Zhou, Xuan; Zheng, Youle; Zuo, Zhicai; Gu, Xiaobin; Yang, Guangyou

doi:10.1080/23802359.2019.1675545

Cited by 5 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The complete mitochondrial genome of A. ovis was 14,205 bp in size with 72.0% AT and encoded 12 protein-coding genes, 22 tRNA genes, and two rRNA genes. All genes were present on the same strand and unidirectionally transcribed, typical for other roundworms characterized so far (Park et al 2011;Xie et al 2011Xie et al , 2013Xie et al , 2019Liu et al 2012). Nine of the 12 protein-coding genes were predicted to use the TAA or TAG (atp6, cytb, cox2-3, nad1, nad4, and nad6) as the stop codons, while the remaining genes (cox1, nad2, and nad5) were deduced to end with an incomplete codon 'T' or 'TA'.…”

mentioning

confidence: 94%

“…Nine of the 12 protein-coding genes were predicted to use the TAA or TAG (atp6, cytb, cox2-3, nad1, nad4, and nad6) as the stop codons, while the remaining genes (cox1, nad2, and nad5) were deduced to end with an incomplete codon 'T' or 'TA'. Twenty-two tRNA genes ranged in size from 51 bp (tRNA (AGN) -Ser and tRNA-Thr) to 61 bp (tRNA-Lys, tRNA-Ile, and tRNA-Met) and had distinctly different stem-loop structures when compared to those of other metazoan mtDNAs (Park et al 2011;Liu et al 2012;Xie et al 2013;Xie et al 2019). Two rRNAs, the small rRNA (rrnS; 692 bp) and large (rrnL; 960 bp) subunits, were located between tRNA-Glu and tRNA (UCN) -Ser and between tRNA-His and nad3, respectively.…”

mentioning

confidence: 99%

“… 2013 ; Xie et al. 2019 ). Two rRNAs, the small rRNA ( rrn S; 692 bp) and large ( rrn L; 960 bp) subunits, were located between tRNA-Glu and tRNA (UCN) -Ser and between tRNA-His and nad3 , respectively.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The mitochondrial genome of the sheep roundworm Ascaris ovis (Ascaridida: Nematoda) from Southwest China

Chen

Wang

Zhou

et al. 2021

Mitochondrial DNA Part B

Self Cite

View full text Add to dashboard Cite

The Ascaris roundworms (Ascaridida: Nematoda), one of the commonest soil-transmitted helminths (STHs), can cause ascariasis with significant socioeconomic and public health impact. In this study, the mitochondrial genome of Ascaris ovis, a representative of this genus from the sheep in Southwest China was determined using Illumina sequencing technology. The assembled genome was 14,205 bp in size and encoded 36 genes, including 12 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. Phylogenetic analysis showed that A. ovis grouped with the congeneric Ascaris lumbricoides of humans, Ascaris spp. of non-human primates and Ascaris suum of pigs and together formed a monophyletic group relationship with either species of Baylisascaris/Toxascaris/Parascaris, species of Toxocara, species of Anisakis/Pseudoterranova or species of Ascaridia/Heterakis in the order Ascaridida, supporting its genetic similarity with A. lumbricoides, A. suum, and other congeneric species. The cumulative mitochondrial DNA data of this genus should contribute to a better understanding of the phylogenetic relationships among these roundworms.

show abstract

mentioning

confidence: 94%

mentioning

confidence: 99%

See 1 more Smart Citation

The mitochondrial genome of the sheep roundworm Ascaris ovis (Ascaridida: Nematoda) from Southwest China

Chen

Wang

Zhou

et al. 2021

Mitochondrial DNA Part B

Self Cite

View full text Add to dashboard Cite

show abstract

“…The mitogenome of the tiger T. leonina was 14,248 bp in size and encoded 12 protein-coding genes (PCGs), 22 tRNAs, and two rRNAs. Similar to congeneric species including those from the dog and cheetah (Liu et al 2014;Jin et al 2019;Xie et al 2019), all of these genes were located on the same strand and transcribed in one direction. Twelve PCGs, except nad2 and nad5 genes deduced to use an incomplete stop codon 'T', were predicted to use the typical TAG as the stop codons.…”

Section: Mitogenome Announcementmentioning

confidence: 88%

The complete mitogenome ofToxascaris leoninafrom the Siberian tiger (Panthera tigris altaica)

Liu

Wang

et al. 2021

Mitochondrial DNA Part B

Self Cite

View full text Add to dashboard Cite

Toxascaris leonina is a polyxenical parasite and commonly found in canids and felids. In this study, we used the Illumina high throughput sequencing and assembly to determine the complete mitogenome of a representative of this parasite from the Siberian tiger (Panthera tigris altaica). The genome was 14,248 bp in size and encoded 12 protein-coding genes, 22 transfer RNAs, and two ribosomal RNAs. Phylogeny showed that two canid (dog)-originated T. leonina were phylogenetically distinct from two felid-originated T. leonina (tiger isolate and cheetah isolate), suggesting at least two distinct subclades of T. leonina infecting these hosts and supporting once again that T. leonina represents a species complex. Furthermore, four isolates of T. leonina grouped together and were more closely related to other species from the family Ascarididae than species of families Toxocaridae, Anisakidae and Ascaridiidae, demonstrating phylogenetic stability of these paraphyletic groups characterized in this study. These cumulative mitochondrial DNA data provide a better understanding of phylogenetic relationships of this polyxenical and zoonotic roundworm species.

show abstract

“…It is clear that T. leonina is a cosmopolitan and polyxenical parasite in wild and domestic canids and felids [1]. However, recent studies by Fogt-Wyrwas et al [12], Jin et al [11] and Xie et al [61] consistently pointed out that T. leonina may be a species complex. Because combined molecular evidence from ribosomal nuclear DNA strongly showed the separation of T. leonina from different hosts into three distinct clades, i.e.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences

Xie

et al. 2020

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

Background: Despite the public health importance of toxocariasis/toxascariasis, only a few species of these ascaridoid parasites from wild canine and feline carnivores have been studied at the molecular level so far. Poor understanding of diversity, host distribution and the potential (zoonotic) transmission of the ascaridoid species among wild animals negatively affects their surveillance and control in natural settings. In this study, we updated previous knowledge by profiling the genetic diversity and phylogenetic relationships of ascaridoid species among eleven wild canine and feline animals on the basis of a combined analysis of the ribosomal internal transcribed spacer region (ITS) gene and the partial mitochondrial cytochrome c oxidase subunit 2 (cox2) and NADH dehydrogenase subunit 1 (nad1) genes. Results: In total, three genetically distinct ascaridoid lineages were determined to be present among these wild carnivores sampled, including Toxocara canis in Alopex lagopus and Vulpes vulpes, Toxocara cati in Felis chaus, Prionailurus bengalensis and Catopuma temmincki and Toxascaris leonina in Canis lupus, Panthera tigris altaica, Panthera tigris amoyensis, Panthera tigris tigris, Panthera leo and Lynx lynx. Furthermore, it was evident that T. leonina lineage split into three well-supported subclades depending on their host species, i.e. wild felids, dogs and wolves and foxes, based on integrated genetic and phylogenetic evidence, supporting that a complex of T. leonina other than one species infecting these hosts. Conclusions: These results provide new molecular insights into classification, phylogenetic relationships and epidemiological importance of ascaridoids from wild canids and felids and also highlight the complex of the taxonomy and genetics of Toxascaris in their wild and domestic carnivorous hosts.

show abstract

Characterization of the complete mitochondrial genome sequence of the dog roundwormToxascaris leonina(Nematoda, Ascarididae) from China

Cited by 5 publications

References 14 publications

The mitochondrial genome of the sheep roundworm Ascaris ovis (Ascaridida: Nematoda) from Southwest China

The mitochondrial genome of the sheep roundworm Ascaris ovis (Ascaridida: Nematoda) from Southwest China

The complete mitogenome ofToxascaris leoninafrom the Siberian tiger (Panthera tigris altaica)

Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences

Contact Info

Product

Resources

About