Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily

Ahmad, Awais Ali; Yang, Xuefei; Zhang, Ting; Wang, Chunqun; Zhou, Caixian; Yan, Xingrun; Hassan, Mubashar; Ikram, Muhammad; Hu, Min

doi:10.3390/genes10020107

Cited by 10 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sequences which were adjusted vaguely were evaluated by a recently portrayed strategy [35]. Phylogenetic evaluation was steered by neighbor joining (NJ), maximum likelihood (ML), and maximum parsimony (MP) techniques as indicated by an earlier depicted strategy [36,37,38]. The program FigTree v. 1.4 () was utilized to develop the phylograms.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Complete Mitochondrial Genome of a Whipworm Trichuris skrjabini (Nematoda: Trichuridae)

Ahmad

Shabbir

Yang

et al. 2019

Genes

Self Cite

View full text Add to dashboard Cite

The complete mitochondrial (mt) genome of Trichuris skrjabini has been determined in the current study and subsequently compared with closely related species by phylogenetic analysis based on concatenated datasets of mt amino acid sequences. The whole mt genome of T. skrjabini is circular and 14,011 bp in length. It consists of a total of 37 genes including 13 protein coding genes (PCGs), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNAs) genes, and two non-coding regions. The gene arrangement and contents were consistent with other members of the Trichuridae family including Trichuris suis, Trichuris trichiura, Trichuris ovis, and Trichuris discolor. Phylogenetic analysis based on concatenated datasets of amino acids of the 12 PCGs predicted the distinctiveness of Trichuris skrjabini as compared to other members of the Trichuridae family. Overall, our study supports the hypothesis that T. skrjabini is a distinct species. The provision of molecular data of whole mt genome of T. skrjabini delivers novel genetic markers for future studies of diagnostics, systematics, population genetics, and molecular epidemiology of T. skrjabini.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of the Complete Mitochondrial Genome of a Whipworm Trichuris skrjabini (Nematoda: Trichuridae)

Ahmad

Shabbir

Yang

et al. 2019

Genes

Self Cite

View full text Add to dashboard Cite

show abstract

“…Insect mitogenomes are characterized by several features shared with most eukaryote mitogenomes, such as a double-strand circular structure, the small genome size (approximately 14–21 K), conserved gene content (13 PCGs, 22 tRNAs and 2 rRNAs), and a lack of extensive recombination [2,3,4]. The complete mitogenome can provide genomic features such as complete genome size, the relative position of genes, RNA secondary structure, and replication and transcription of control patterns [5,6]. Its characterization can help us explore evolutionary patterns across species.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Complete Mitochondrial Genome of Harpalus sinicus and Its Implications for Phylogenetic Analyses

Tan

Zhang

et al. 2019

Genes

View full text Add to dashboard Cite

In this study, we report the complete mitochondrial genome of Harpalus sinicus (occasionally named as the Chinese ground beetle) which is the first mitochondrial genome for Harpalus. The mitogenome is 16,521 bp in length, comprising 37 genes, and a control region. The A + T content of the mitogenome is as high as 80.6%. A mitochondrial origins of light-strand replication (OL)-like region is found firstly in the insect mitogenome, which can form a stem-loop hairpin structure. Thirteen protein-coding genes (PCGs) share high homology, and all of them are under purifying selection. All tRNA genes (tRNAs) can be folded into the classic cloverleaf secondary structures except tRNA-Ser (GCU), which lacks a dihydrouridine (DHU) stem. The secondary structure of two ribosomal RNA genes (rRNAs) is predicted based on previous insect models. Twelve types of tandem repeats and two stem-loop structures are detected in the control region, and two stem-loop structures may be involved in the initiation of replication and transcription. Additionally, phylogenetic analyses based on mitogenomes suggest that Harpalus is an independent lineage in Carabidae, and is closely related to four genera (Abax, Amara, Stomis, and Pterostichus). In general, this study provides meaningful genetic information for Harpalus sinicus and new insights into the phylogenetic relationships within the Carabidae.

show abstract

“…For example, it is possible that the moderate differences observed within linages will become more evident as different host subspecies are sampled, as has happened with other Trypanoxyuris species (Solórzano-García et al, 2019); or that the species assemblages occurring in each population vary across the host range. Another possible approach might require the use of next generation sequencing (NGS) methods, gathering large molecular datasets (e.g., complete mitochondrial genomes) that could result in a more robust species delimitation (Ahmad et al, 2019; Duan et al, 2015; Gao et al, 2014; Jaleta et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle

Solórzano‐García

Ospina

Rondón

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

Pinworms of primates are believed to be highly host specific parasites, forming co-evolutionary associations with their hosts. In order to assess the strength and reach of such evolutionary links, we need to have a broad understanding of the pinworm diversity associated with primates. Here, we employed an integrative taxonomic approach to assess pinworm diversity in red howler monkeys in Colombia. Molecular and morphological evidence validate the presence of at least four different species of Trypanoxyuris occurring in red howler monkeys: T. minutus, a widely distributed species, and three new species, T. seunimiii n. sp., T. kemuimae n. sp. and T. kotudoi n. sp. The mitochondrial COI gene and the 28S ribosomal gene were used for phylogenetic assessments through Bayesian inference. The three new species were morphologically distinct and formed reciprocally monophyletic lineages. Further molecular lineage subdivision in T. minutus and T. kotudoi n. sp. without morphological correspondence, suggests the potential scenario for the existence of cryptic species. Phylogenetic relationships imply that the different species of Trypanoxyuris occurring in each howler monkey species were acquired through independent colonization events. On-going efforts to uncover pinworm diversity will allow us to test the degree of host specificity and the co-phylogenetic hypothesis, as well as to further unravel the primate-pinworm evolutionary history puzzle.

show abstract

Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily

Cited by 10 publications

References 53 publications

Characterization of the Complete Mitochondrial Genome of a Whipworm Trichuris skrjabini (Nematoda: Trichuridae)

Characterization of the Complete Mitochondrial Genome of a Whipworm Trichuris skrjabini (Nematoda: Trichuridae)

Characterization of the Complete Mitochondrial Genome of Harpalus sinicus and Its Implications for Phylogenetic Analyses

Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle

Contact Info

Product

Resources

About