Characterization of the complete chloroplast genome of the Musk Larkspur <i>Delphinium brunonianum</i> (Ranunculales: Ranunculaceae)

Li, Qien; Guo, Xiao; Yuan, Farong; Nima, Cairang; Dongzhi, Duojie; Duojie,; Li, XianJia

doi:10.1080/23802359.2020.1775522

Cited by 2 publications

(5 citation statements)

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“…genome sequenced here, all contained a total of 129 genes (including 111 unique genes), with the total GC content ranging from 38.13% to 38.30% (Table 1). However, compared with the previously published plastomes of other seven taxa in Delphinium [35][36][37], some taxa were found to contain different numbers of genes in different samples, for instance, D. anthriscifolium (MK253461), D. brunonianum (NC_051554), D. candelabrum var. monanthum (MW246165), D. ceratophorum (MK253460), D. maackianum Regel (NC_047293) and D. yunnanense (MW246156) were reported contained 128, 131, 130, 128, 129 and 130 genes, respectively, whereas all annotated here contained only 129 genes.…”

Section: Plastome Features In Delphiniummentioning

confidence: 69%

“…monanthum (Hand.-Mazz.) W. T. Wang MW246165 153,995 38.25 84,862 26,543 16,047 129 (111) 84 (77) 37 (30) 8 (4) [ 36 ] D. ceratophorum Franch. MK253460 154,245 38.27 84,801 26,560 16,324 129 (111) 84 (77) 39 (31) 8 (4) [ 35 ] D. elatum var.…”

Section: Resultsmentioning

confidence: 99%

“…(*) OR263590 154,255 38.25 84,765 26,594 16,302 129 (111) 84 (77) 37 (30) 8 (4) This study D. shawurense W. T. Wang (*) OR263585 154,284 38.26 84,820 26,561 16,342 129 (111) 84 (77) 37 (30) 8 (4) This study D. winklerianum Huth (*) OR263589 154,235 38.26 84,754 26,594 16,293 129 (111) 84 (77) 37 (30) 8 (4) This study D. yunnanense Franch. MW246156 154,053 38.26 84,639 26,551 16,312 129 (111) 84 (77) 37 (30) 8 (4) [ 36 ] …”

Section: Resultsmentioning

confidence: 99%

“…However, currently, only seven Chinese Delphinium cp. genomes were available (https://www.ncbi.nlm.nih.gov), which limited our knowledge of the organization and evolution of plastomes in Delphinium and the tribe Delphinieae [34][35][36][37][38].…”

Section: Discussionmentioning

confidence: 99%

“…These eight cp. genomes were combined and compared with previously reported plastomes of six other Chinese Delphinium species [33][34][35][36][37]. The aims were: (1) to present the structure of cp.…”

Section: Discussionmentioning

confidence: 99%

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Complete chloroplast genomes of eight Delphinium taxa (Ranunculaceae) endemic to Xinjiang, China: insights into genome structure, comparative analysis, and phylogenetic relationships

Song,

Zhu,

2024

BMC Plant Biol

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Background Delphinium L. represents a taxonomically intricate genus of significant phylogenetic and economic importance in Ranunculaceae. Despite the existence of few chloroplast genome datasets, a comprehensive understanding of genome structures and selective pressures within the genus remains unknown. Furthermore, several taxa in this genus are exclusively found in Xinjiang, China, a region renowned for its distribution and diversity of Chinese and Central Asian Delphinium species. Therefore, investigating the features of chloroplast genomes in this area will provide valuable insights into the evolutionary processes and phylogenetic relationships of the genus. Results In this study, the eight newly completed chloroplast genomes are examined, ranging in length from 153,979 bp to 154,284 bp. Alongside these, analysing six previously reported taxa re-annotated in Delphinium, 111 unique genes are identified across all samples. Genome structure, distributions of simple sequence repeats and short dispersed repeats, as well as gene content are similar among these Delphinium taxa. Nine hypervariable intergenic spacers and protein coding regions, including ndhF-trnL(TAG), rpl16-intron, rpl33, rps15, rps18, trnK(TTT)-trnQ(TTG), trnP(TGG)-psaJ, trnT(GGT)-psbD and ycf1, are identified among 13 perennial Delphinium. Selective pressure and codon usage bias of all the plastid genes are performed within 14 Delphinium taxa. Phylogenetic analysis based on 14 Delphinium plastomes, alongside two Aconitum (Ranunculaceae) species serving as outgroup taxa, reveals the monophyletic nature of Delphinium. Our findings further discern Delphinium into two distinct clades: perennial species (clade I) and annual species (clade II). In addition, compared with the nrDNA ITS topology, cytological data and morphological characters, D. mollifolium and D. maackianum showed potential involvement in hybridization or polyploidization processes. Excluding these two species, the perennial Delphinium (clade I) exhibits a stronger consistency with the morphology-based system that utilized seed morphology. Conclusion This study represents the first comprehensive analysis of plastomic variations among Delphinium taxa, based on the examination of 14 complete plastomes. The chloroplast genome structure of Delphinium is similar to other angiosperms and possesses the typical quadripartite structure with the conserved genome arrangement and gene features. In addition, the variation of non-coding regions is larger than coding regions of the chloroplast genome. Through DNA sequence divergence across Delphinium plastomes and subsequent phylogenomic analyses ndhF-trnL(TAG) and ycf1 are identified as promising molecular markers. These highly variable loci held significant potential for future phylogenetic and phylogeographic studies on Delphinium. Our phylogenomic analyses based on the whole plastomes, concatenation of 132 unique intergenic spacer regions, concatenation of 77 unique protein-coding genes and nrDNA ITS, all support the monophyly of Delphinium and perennial taxa clusters together into one clade within this genus. These findings provide crucial data for systematic, phylogenomic and evolutionary research in the genus for future studies.

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Section: Plastome Features In Delphiniummentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Complete chloroplast genomes of eight Delphinium taxa (Ranunculaceae) endemic to Xinjiang, China: insights into genome structure, comparative analysis, and phylogenetic relationships

Song,

Zhu,

2024

BMC Plant Biol

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Delphinium as a model for development and evolution of complex zygomorphic flowers

Sharma,

Pandher,

Alcaraz Echeveste

et al. 2024

Front. Plant Sci.

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The complex zygomorphic flowers of the early-diverging eudicot Delphinium provide an opportunity to explore intriguing evolutionary, developmental, and genetic questions. The dorsal perianth organs, consisting of a spurred sepal and the nectar-bearing spurred petal(s) in Delphinium, contribute to the dorso-ventralization and zygomorphic flower morphology. The seamless integration of the two or three dorsal petaloid spurred organs is considered a synorganization, and the resulting organ complex is referred to as a hyperorgan. The hyperorgan shows variability within the tribe due to variation in the number, size, and shape of the spurs. Research in recent decades within this tribe has enhanced our understanding of morphological evolution of flowers. More recently, functional studies using the RNAi approach of Virus-Induced Gene Silencing (VIGS) have unraveled interesting results highlighting the role of gene duplication in the functional diversification of organ identity and symmetry genes. Research in this early-diverging eudicot genus bridges the gaps in understanding the morphological innovations that are mostly studied in model grass and core eudicot clades. This first comprehensive review synthesizes eco-evo-devo research on Delphinium, developing a holistic understanding of recent advancements and establishing the genus as an exceptional model for addressing fundamental questions in developmental genetics, particularly in the evolution of complex flowers. This progress highlights Delphinium’s significant potential for future studies in this field.

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Characterization of the complete chloroplast genome of the Musk Larkspur Delphinium brunonianum (Ranunculales: Ranunculaceae)

Cited by 2 publications

References 14 publications

Complete chloroplast genomes of eight Delphinium taxa (Ranunculaceae) endemic to Xinjiang, China: insights into genome structure, comparative analysis, and phylogenetic relationships

Complete chloroplast genomes of eight Delphinium taxa (Ranunculaceae) endemic to Xinjiang, China: insights into genome structure, comparative analysis, and phylogenetic relationships

Delphinium as a model for development and evolution of complex zygomorphic flowers

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