Characterization of the complete chloroplast genome of a purple passion flower variety ‘Pingtang No.1’ (Passiflora edulia Sims) in China and phylogenetic relationships

Abstract

Passion flower (Passiflora edulia Sims) is an important fruit that is of great economic importance. In this study, we presented the chloroplast genome of a purple passion flower variety 'Pingtang No.1' in China using BGISEQ-500 sequencing. Its chloroplast genome is 152,621 bp in size. It contains a pair of inverted repeat regions of 25,989 bp, each separating a small single copy region of 13,352 bp and a large single copy region of 85,141 bp. Totally, 111 unique genes, including 77 protein coding genes, 30 tRNAs, and 4 rRNAs, were identified and annotated in the chloroplast genome. Phylogenetic maximum likelihood analysis based on chloroplast genomes of 35 plant species, mainly from the genus Passiflora indicated that 'Pingtang No.1' and yellow passion flower 'IAPAR-123' (Passiflora edulis) cluster together. The chloroplast genome can be used for a better understanding of the evolutionary relationships of plant species of the family Passifloraceae, especially the genus Passiflora. ARTICLE HISTORY

Characterization of complete chloroplast genome of artificial hybrid passion fruit ‘Ziyan’, Passiflora edulis Sims × P. edulis f. edulis Sims (Passifloraceae)

“…The bootstrap values were calculated using 1000 replicates (Stamatakis 2014). As shown in Figure 1, phylogenetic analysis revealed that 'Ziyan' is clustered with the yellow passion flower 'IAPAR-123', within Passifloraceae, which is in agreement with a previous study (Yang et al 2019).…”

“…In the current market, yellow and purple passion fruits are both sold as the main varieties. The entire chloroplast (cp) genomes of yellow and purple passion fruits were reported by Cauz-Santos et al (2017) and Yang et al (2019). However, there is no complete chloroplast genome information for their hybrid.…”

Characterization of the complete chloroplast genome of Houttuynia cordata Thunb and phylogenetic relationships

“…The specimen sample of H. cordata Thunb was isolated from Teaching Experiment Farm of Guizhou University, Guiyang, Guizhou Province, China (26 21 0 29 00 N; 106 41 0 01 00 E) and sample was deposited at Guizhou University. Total genomic DNA was extracted from fresh leaves according to my previous study (Yang et al 2019), stored at the Guizhou University (No. YXC01) and was used for the shotgun library construction and BGISEQ-500 sequencing to generate about 1 Gbp data.…”

“…Total genomic DNA was extracted from fresh leaves according to my previous study (Yang et al. 2019 ), stored at the Guizhou University (No. YXC01) and was used for the shotgun library construction and BGISEQ-500 sequencing to generate about 1 Gbp data.…”

Characterization of the complete chloroplast genome of Aster subulatus Michx

“…Total genomic DNA was extracted from fresh leaves according to the method prescribed by Yang et al. ( 2019 ) and stored at the Guizhou University (No. JDC01).…”