Characterization of the co-agonist effects of strontium and calcium on myo-inositol trisphosphate-dependent ion fluxes in cerebellar microsomes

Hannaert-Merah, Zalika; Combettes, Laurent; Coquil, Jean-François; Swillens, Stéphane; Mauger, Jean‐Pierre; Claret, M; Champeil, Philippe

doi:10.1016/0143-4160(95)90054-3

Cited by 28 publications

(42 citation statements)

References 44 publications

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“…Data in Figure 1 $ and various concentrations of unlabelled ligand. As in previous studies [14,33] (Figures 2A and 2B (Figure 3), although at a much lower concentration than type 1 receptors. …”

Section: Nm [$H]inspsupporting

confidence: 85%

“…Equilibrium $ (approx. 35 %) was lower than reported in previous studies (65 %) [33]. This led us to investigate the influence of our experimental conditions on Ca# + inhibition.…”

Section: Figure 1 Presence Of a Micromolar Concentration Of Free Ca 2mentioning

confidence: 53%

“…Other experimental conditions were as described in the legend to Figure 1 for Ca# + . However, these sites exhibit different affinities for Mn# + and Sr# + [20][21][22]. In the present study we determined the selectivity of the Ca# + -binding site responsible for the inhibition of [$H]InsP $ binding to cerebellar membranes by comparing the effects of these cations on this binding.…”

Section: Figure 6 Lack Of Inhibition Of [ 3 H]insp 3 Binding By Buffementioning

confidence: 96%

“…$ binding was measured at 20 mC by using a perfusion protocol similar to that described before [14,33]. A 120 µl aliquot of membrane preparation was diluted 1 : 10 in buffer A at 20 mC; 1 ml of the resulting suspension, containing 200-500 µg of protein, was layered on a glass-fibre filter (GF\C).…”

Section: [$H]inspmentioning

confidence: 99%

“…The selectivities of stimulation and inhibition of IICR for bivalent cations differ [20][21][22], suggesting that different sites are involved. Ca# + -binding regions have been identified in type 1 [23][24][25] and type 2 InsP $ Rs [23] but their function is unknown.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of cerebellar Ins(1,4,5)P3 receptor by interaction between Ins(1,4,5)P3 and Ca2+

Coquil

Picard

Mauger

1999

Biochemical Journal

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We have characterized in detail the Ca# + -dependent inhibition of [$H]Ins(1,4,5)P $ ([$H]InsP $ ) binding to sheep cerebellar microsomes, over a short duration (3 s), with the use of a perfusion protocol. This procedure prevented artifacts previously identified in studies of this Ca# + effect. In a cytosol-like medium at pH 7.1 and 20 mC, a maximal inhibition of approx. 50 % was measured. Both inhibition and its reversal were complete within 3 s. Ca# + decreased the affinity of the receptor for InsP $ by approx. 50 % (K d 146p24 nM at pCa 9 and 321p56 nM at pCa 5.3), without changing the total number of binding sites. Conversely, increasing the [$H]InsP $ concentration from 30 to 400 nM tripled the IC &! for Ca# + and decreased the maximal inhibition by 63 %. This is similar to a partial competitive inhibition between InsP $ binding and inhibitory Ca# + binding and is consistent with InsP $ and

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Section: Nm [$H]inspsupporting

confidence: 85%

“…Equilibrium $ (approx. 35 %) was lower than reported in previous studies (65 %) [33]. This led us to investigate the influence of our experimental conditions on Ca# + inhibition.…”

Section: Figure 1 Presence Of a Micromolar Concentration Of Free Ca 2mentioning

confidence: 53%

Section: Figure 6 Lack Of Inhibition Of [ 3 H]insp 3 Binding By Buffementioning

confidence: 96%

Section: [$H]inspmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of cerebellar Ins(1,4,5)P3 receptor by interaction between Ins(1,4,5)P3 and Ca2+

Coquil

Picard

Mauger

1999

Biochemical Journal

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Relative and combined effects of ethanol and protein deficiency on strontium and barium bone content and fecal and urinary excretion

González‐Reimers

Rodríguez-Moreno

Martı́nez-Riera

et al. 1999

Biol Trace Elem Res

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Strontium metabolism has attracted considerable interest because of to its interaction with calcium, the bone alterations detected after treatment with strontium, and its potential value as a paleodietary indicator. The effects of ethanol on strontium and barium metabolism-another divalent cation which also accumulates in bone--is largely unknown. Based on this fact, we have determined bone content and fecal and urinary excretion of Ba and Sr in four groups of eight animals each pair-fed for 8 wk with (1) a nutritionally adequate diet, (2) a 36% (as energy) ethanol-containing isocaloric diet, (3) a 2% protein, isocaloric diet, and (4) a 36% ethanol, 2% protein isocaloric diet, following the Lieber-DeCarli model. Five additional rats were fed with the control diet ad libitum. We have found that ethanol tends to decrease and a low protein diet to increase bone strontium content; the decrease in bone strontium in the ethanol-fed rats is accompanied by an increase in the absolute excretion of strontium in urine. Ethanol also decreases bone barium content, but the effect of ethanol on urinary barium excretion is opposite that of strontium, a decrease. Thus, we conclude that ethanol alters both barium and strontium metabolism and bone deposition.

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Ion channels in smooth muscle: Regulation by the sarcoplasmic reticulum and mitochondria

et al. 2007

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Characterization of the co-agonist effects of strontium and calcium on myo-inositol trisphosphate-dependent ion fluxes in cerebellar microsomes

Cited by 28 publications

References 44 publications

Regulation of cerebellar Ins(1,4,5)P3 receptor by interaction between Ins(1,4,5)P3 and Ca2+

Regulation of cerebellar Ins(1,4,5)P3 receptor by interaction between Ins(1,4,5)P3 and Ca2+

Relative and combined effects of ethanol and protein deficiency on strontium and barium bone content and fecal and urinary excretion

Ion channels in smooth muscle: Regulation by the sarcoplasmic reticulum and mitochondria

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