Characterization of the Cloned Atlantic Cod Neuropeptide Y-Yb Receptor: Peptide-Binding Requirements Distinct from Known Mammalian Y Receptors

Sharma, Parul; Arvidsson, Ann-Kristin; Wraith, Amanda; Beck‐Sickinger, Annette G.; Jönsson‐Rylander, Ann‐Cathrine; Larhammar, Dan

doi:10.1006/gcen.1999.7332

Cited by 12 publications

(6 citation statements)

References 33 publications

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“…The binding properties of the zYb and zYc receptors most resemble those of Y 1 , whereas Ya display a strikingly indiscriminate binding profile (2,35). The cod Yb receptor shows a binding profile that is similar to both zYb and zYc (37). The pharmacological results from our studies, with only a small difference in effect of porcine [Leu 31 ,Pro 34 ]NPY and porcine NPY- , may suggest an interaction with a (hitherto uncloned) receptor more like the zYa than the cod Yb receptor (2, 37), but an interaction with a mixture of the cod Yb receptor and a zYa-like receptor in the cod vasculature cannot be excluded.…”

Section: Discussionmentioning

confidence: 98%

“…These are all structurally distinct from the mammalian NPY receptors. The cod Yb receptor shows 50% identity to Y 1 , Y 4 , and y 6 and only 30% to Y 2 and Y 5 (37). The binding properties of the zYb and zYc receptors most resemble those of Y 1 , whereas Ya display a strikingly indiscriminate binding profile (2,35).…”

Section: Discussionmentioning

confidence: 98%

“…The Y 1 receptor subfamily lineage includes several gene duplications, and different subtypes have evolved in different vertebrate groups. In teleost fish, three or even four duplications have led to zYa, zYb, and zYc receptors in zebrafish (27,34,38) and a Yb receptor in cod (1,37). These are all structurally distinct from the mammalian NPY receptors.…”

Section: Discussionmentioning

confidence: 99%

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Neuropeptide Y effects on vasorelaxation and intestinal contraction in the Atlantic cod Gadus morhua

Shahbazi

Holmgren

Larhammar

et al. 2002

American Journal of Physiology-Regulatory, Integrative and Comp

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Neuropeptide Y (NPY) has prominent cardiovascular effects in mammals and sharks, but no such effect has previously been demonstrated in any teleost fish. In the Atlantic cod, we found that cod NPY (10(-10)-10(-6) M) relaxed celiac arteries precontracted with epinephrine, and weak contractions were elicited in intestinal ring preparations. A few NPY-immunoreactive nerve fibers were present along small gut arteries. The results suggest that cod NPY produces vasorelaxation both by a direct action on smooth muscle and by release of prostaglandins, but with no involvement of nitric oxide, leukotrienes, or endothelium-derived relaxing factors. An additional indirect effect involving another neurotransmitter may occur. Cod NPY (10(-7) M) and human NPY (10(-7) M) had identical effects on the vessels. Small differences only in the effects of porcine [Leu(31),Pro(34)]NPY, NPY-(13-36), and cod NPY suggest the presence of a Y(1) subfamily receptor, similar to the zebrafish Ya receptor. A physiological role for NPY in teleost vasculature is concluded, but surprisingly the effect, a vasodilation, is opposite to that in mammals and is mediated by prostaglandins.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Neuropeptide Y effects on vasorelaxation and intestinal contraction in the Atlantic cod Gadus morhua

Shahbazi

Holmgren

Larhammar

et al. 2002

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Binding competition assays with Y1 and Y2 agonists suggested that the dogfish receptor closely resembles the mammalian Y1‐receptor subtype (McVey et al, 1996). Although the Y1‐receptor subtype has yet been not characterized in non‐tetrapod vertebrates, three receptor subtypes have been cloned in teleost fish: Ya (Starbäck et al, 1999), Yb (Lundell et al, 1997; Arvidsson et al, 1998; Sharma et al, 1999), and Yc (Ringvall et al, 1997). The pharmacological profile of both the Yb and the Yc receptor is similar to mammalian Y1, whereas Ya resembles the Y5 feeding receptor even though these two receptors shared an amino acid identity of only 27% (Starbäck et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Peptide YY (PYY) and fish pancreatic peptide Y (PY) expression in the brain of the sea bass (Dicentrarchus labrax) as revealed by in situ hybridization

et al. 2000

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Tetrapod vertebrates express three neuropeptide Y (NPY)-related peptides: NPY, peptide YY (PYY), and pancreatic polypeptide (PP). Both NPY and PYY mRNA have been localized in the brain of tetrapods whereas PP expression is restricted to the pancreas. Some teleost fish commonly produce NPY and PYY but pancreatic peptide Y (PY) instead of PP. Both NPY and PYY mRNAs are widely distributed in the brain of non-tetrapod species, but no information about PY central expression is available. In the present study, molecular riboprobes were used to study PYY and PY mRNA central distribution in the sea bass (Dicentrarchus labrax). PYY and PY gene expression was predominantly detected within the sea bass forebrain. Telencephalic PYY gene expression was restricted to the ventral part of the ventral telencephalon, and no PY expression was detected in the cerebral hemispheres. Both PYY and PY mRNAs were found within the preoptic area and lateral hypothalamus. Distinct PY or PYY mRNA cell groups were localized in the pretectal area and synencephalon or posterior tubercle, respectively. Caudally, PY gene expression was found in the medial reticular formation, whereas PYY transcripts were localized within the vagal lobe. The results demonstrate that vertebrate brain expresses three NPY-related genes and further support the hypothesis that PP and PY arose by independent gene duplications from PYY. The receptor system of the NPY family as well as gene expression within the main hypophysiotropic and feeding behavior areas suggest an involvement of both peptides in the control of food intake and pituitary secretion.

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“…The third subtype, Yc, may possibly be an orthologue of mammalian y6 (Ringvall et al, 1997; Starbäck et al., 1999). We have also characterized the Yb receptor in Atlantic cod (Arvidsson et al, 1998; Sharma et al, 1999). Recently, we have identified a Y2‐like receptor in zebrafish (R. Fredriksson and D. Larhammar, unpublished data) and a Y1‐like gene in sturgeon (E. T. Larson and D. Larhammar, unpublished data).…”

mentioning

confidence: 99%

Zebrafish Genes for Neuropeptide Y and Peptide YY Reveal Origin by Chromosome Duplication from an Ancestral Gene Linked to the Homeobox Cluster

Söderberg¹,

Wraith²,

Ringvall³

et al. 2000

Journal of Neurochemistry

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Neuropeptide Y (NPY) and peptide YY (PYY) are related 36-amino acid peptides. NPY is widely distributed in the nervous system and has several physiological roles. PYY serves as an intestinal hormone as well as a neuropeptide. We report here cloning of the npy and pyy genes in zebrafish (Danio rerio). NPY differs at only one to four amino acid positions from NPY in other jawed vertebrates. Zebrafish PYY differs at three positions from PYY from other fishes and at 10 positions from mammals. In situ hybridization showed that neurons containing NPY mRNA have a widespread distribution in the brain, particularly in the telencephalon, optic tectum, and rhombencephalon. PYY mRNA was found mainly in brainstem neurons, as reported previously for vertebrates as divergent as the rat and the lamprey, suggesting an essential role for PYY in these neurons. PYY mRNA was observed also in the telencephalon. These results were confirmed by immunocytochemistry. As in the human, the npy gene is located adjacent to homeobox (hox) gene cluster A (copy a in zebrafish), whereas the pyy gene is located close to hoxBa. This suggests that npy and pyy arose from a common ancestral gene in a chromosomal duplication event that also involved the hox gene clusters. As zebrafish has seven hox clusters, it is possible that additional NPY family genes exist or have existed. Also, the NPY receptor system seems to be more complex in zebrafish than in mammals, with at least two receptor genes without known mammalian orthologues.

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Characterization of the Cloned Atlantic Cod Neuropeptide Y-Yb Receptor: Peptide-Binding Requirements Distinct from Known Mammalian Y Receptors

Cited by 12 publications

References 33 publications

Neuropeptide Y effects on vasorelaxation and intestinal contraction in the Atlantic cod Gadus morhua

Neuropeptide Y effects on vasorelaxation and intestinal contraction in the Atlantic cod Gadus morhua

Peptide YY (PYY) and fish pancreatic peptide Y (PY) expression in the brain of the sea bass (Dicentrarchus labrax) as revealed by in situ hybridization

Zebrafish Genes for Neuropeptide Y and Peptide YY Reveal Origin by Chromosome Duplication from an Ancestral Gene Linked to the Homeobox Cluster

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