Characterization of the cellulolytic enzyme produced by Streptomyces griseorubens (Accession No. AB184139) isolated from Indian soil

The goal was to investigate the cellulase enzyme production ability of bacterial strain C1 isolated from cow dung and identified as Bacillus sp. on the basis of 16 S rDNA sequence homology. The effects of different carbon sources like Carboxymethyl cellulose (CMC), avicel, starch, maltose, sucrose, glucose, fructose, galactose and lactose on cellulase production at varying environmental parameters of incubation period (2-10 days), temperature (35-55°C), and pH (6.0-8.5) were examined. The CMC was the best carbon source for cellulase production followed by lactose in this bacterial strain. The maximum enzyme production was achieved at a temperature of 50°C by Bacillus sp. with pH of 7.0 on the 8th day of growth. The nitrogen source NH 4 NO 3 at 0.175% was optimum for cellulase production by this bacterium. A putative mutant (C1M 26 ) was screened from wild C1 strain after mutagenesis with N-methyl-N 0 -nitro-N-nitrosoguanidine (NTG) as a mutagenic agent. The mutant C1M 26 produced a larger amount of cellulase in comparison to wild type C1 strain. ª 2014 Production and hosting by Elsevier B.V. on behalf of King Saud University.

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“…(Sangkharak et al, 2012), Bosea sp. (Sadhu et al, 2012) Streptomyces griseorubens (Prasad et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung

Sadhu

Ghosh

Aditya

et al. 2014

Journal of King Saud University - Science

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“…In this study, temperature was found to influence extracellular enzyme secretion and 35°C was found optimum for cellulase production by Streptomyces sp. In a study by Prasad et al, [29], Streptomyces griseorubens showed highest cellulolytic activity at 45°C on 6 th day of incubation period. In another study, Streptomyces sp.…”

Section: Resultsmentioning

confidence: 94%

Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by <i>Streptomyces</i> Sp

Ishchi¹,

Sibi²

2019

AJBIO

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Cellulases have diversity of industrial applications and their cost effective production using agroindustrial wastes by solid state fermentation poses an efficient method. Actinomycetes are considered highly valuable due to their secondary metabolites production and in this study, an attempt was made to optimize the use of ragi husk and refine the process of cellulase production by temperature mediated solid state fermentation. Actinomycetes were isolated from paper mill industry soil and cellulase producing Streptomyces sp was selected for the experiments. Ragi husk was used as substrate for solid state fermentation of cellulase and varying incubation temperatures (20°C, 25°C, 30°C, 35°C and 40°C) was considered to determine its effect on enzyme activity after 6 th , 9 th and 12 th day of fermentation. The carboxymethyl cellualse (CMC-ase) activity was measured and the observations obtained were compared with the standard glucose curve to determine the amount of reducing sugar (µg ml -1 ) released. Enzyme activity was highest at 35°C and was recorded as 35.14, at the end of 6 th , 9 th and 12 th day of fermentation. Highest amount of reducing sugars at a concentration of 322 µg ml -1 was released at the end of 12 th day at 35°C. The results indicated that the enzyme activity was temperature dependent while using ragi husk as growth substrate under solid state fermentation.

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“…Pemanfaatan lignoselulosa melibatkan proses biokonversi yang dapat dilakukan oleh aktinomiset melalui produksi enzim lignoselulase ektraseluler. Aktinomiset merupakan salah satu mikroba penghasil selulase yang terlibat dalam degradasi utama material berlignoselulosa [7]. Aktinomiset merupakan bakteri Gram positif berfilamen.…”

Section: Pendahuluanunclassified

“…Pembentukan pigmen ditentukan dengan menginokulasikan isolat aktinomiset terpilih masing-masing pada media pepton-yeast extract agar, tyrosine agar, dan tryptone-yeast extract broth. Karakter fisiologi aktinomiset juga diamati dengan pertumbuhan pada media ISP-2 dalam berbagai pH (4)(5)(6)(7)(8). Pertumbuhan pada berbagai sumber karbon dilakukan dengan menginokulasikan aktinomiset pada beberapa sumber karbon (D-glukosa sebagai kontrol positif, L-arabinosa, sukrosa, D-xilosa, inositol, D-manitol, D-fruktosa, rhamnosa dan rafinosa) dalam basal mineral.…”

Section: Identifikasi Fisiologi Dan Biokimia Aktinomisetunclassified

Identifikasi Aktinomiset Selulolitik dan Xilanolitik Indigenous

Utarti

Suwanto²,

Suhartono³

et al. 2020

B. SAINSTEK

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Lignoselulosa merupakan penyusun utama dinding sel tumbuhan, sehingga keberadaannya berlimpah di alam. Aktinomiset indigenous yang memiliki aktivitas selulolitik dan xilanolitik ekstraseluler berpeluang sebagai agens biokonversi limbah berlignoselulosa menjadi produk bermanfaat. Penelitian ini bertujuan untuk mendapatkan aktinomiset potensial yang memiliki aktivitas selulolitik dan xilanolitik. Penelitian ini diawali dari isolasi dan pemurnian aktinomiset indigenous asal lahan perkebunan kelapa sawit dan Taman Nasional Bukit Duabelas Jambi.Tahapan selanjutnya adalah penapisan aktivitas selulolitik dan xilanolitik dari aktinomiset, uji pertumbuhan aktinomiset pada mikrokristalin selulosa, dan identifikasi aktinomiset potensial berdasarkan karakter morfologi, fisiologi dan biokimia. Hasil penelitian menunjukkan bahwa aktinomiset isolat S2 yang diisolasi dari lahan perkebunan kelapa sawit mempunyai aktivitas selulolitik dan xilanolitik lebih baik dari keempat isolat lain. Aktinomiset isolat S2 juga mampu tumbuh secara lebih baik pada mikrokristalin selulosa. Aktivitas selulolitik, xilanolitik dan kemampuan tumbuh pada mikrokristalin selulosa dari aktinomiset isolat S2 menunjukkan potensinya sebagai agens pendegradasi material belignoselulosa. Berdasarkan identifikasi morfologi, fisiologi dan biokimia, aktinomiset isolat S2 tergolong dalam genus Streptomyces.

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Characterization of the cellulolytic enzyme produced by Streptomyces griseorubens (Accession No. AB184139) isolated from Indian soil

Cited by 49 publications

References 13 publications

Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung

Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung

Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by <i>Streptomyces</i> Sp

Identifikasi Aktinomiset Selulolitik dan Xilanolitik Indigenous

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Characterization of the cellulolytic enzyme produced by Streptomyces griseorubens (Accession No. AB184139) isolated from Indian soil

Cited by 49 publications

References 13 publications

Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung

Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung

Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by &lt;i&gt;Streptomyces&lt;/i&gt; Sp

Identifikasi Aktinomiset Selulolitik dan Xilanolitik Indigenous

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Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by <i>Streptomyces</i> Sp