Characterization of the CCD and CMOS cameras for grating-based phase-contrast tomography

Lytaev, Pavel; Hipp, Alexander; Lottermoser, L.; Herzen, Julia; Greving, Imke; Khokhriakov, Igor; Meyer-Loges, Stephan; Plewka, Jörn; Burmester, Jörg; Caselle, M.; Vogelgesang, Matthias; Chilingaryan, S.; Kopmann, A.; Balzer, M.; Schreyer, A.; Beckmann, Felix

doi:10.1117/12.2061389

Cited by 18 publications

(14 citation statements)

References 10 publications

(11 reference statements)

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“…Once trimmed, the first specimen was stained in 1% iodine solution and critical point dried. Synchrotron radiation-based microcomputed tomography scans of this critical point dried sponge were recorded at the Imaging Beamline P05 (IBL; Greving et al, 2014;Haibel et al, 2010;Wilde et al, 2016) operated by the Helmholtz-Zentrum-Geesthacht at the storage ring PETRA III (Deutsches Elektronen Synchrotron -DESY, Hamburg, Germany). Imaging was done at photon energy of 35 keV.…”

Section: Synchrotron Radiation Micro-computed Tomographymentioning

confidence: 99%

“…Imaging was done at photon energy of 35 keV. Projections were recorded with a sample to detector distance of 200 mm using a custom developed 20 MP CMOS camera system (Lytaev et al, 2014) with an effective pixel size of 1.28 μm. For each tomographic scan 3601 projections at equal intervals between 0 and π were recorded.…”

Section: Synchrotron Radiation Micro-computed Tomographymentioning

confidence: 99%

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Integrative anatomical study of the branched annelid Ramisyllis multicaudata (Annelida, Syllidae)

Ponz‐Segrelles

Glasby²,

Helm

et al. 2021

Journal of Morphology

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The sponge‐dwelling Syllidae Ramisyllis multicaudata and Syllis ramosa are the only annelid species for which a branched body with one head and multiple posterior ends is known. In these species, the head is located deep within the sponge, and the branches extend through the canal system of their host. The morphology of these creatures has captivated annelid biologists since they were first discovered in the late XIXth century, and their external characteristics have been well documented. However, how their branched bodies fit within their symbiotic host sponges and how branches translate into internal anatomy has not been documented before. These features are crucially relevant for understanding the body of these animals, and therefore, the aim of this study was to investigate these aspects. In order to assess these questions, live observation, as wells as histology, immunohistochemistry, micro‐computed tomography, and transmission electron microscopy techniques were used on specimens of R. multicaudata. By using these techniques, we show that the complex body of R. multicaudata specimens extends greatly through the canal system of their host sponges. We demonstrate that iterative external bifurcation of the body is accompanied by the bifurcation of the longitudinal organ systems that are characteristic of annelids. Additionally, we also highlight that the bifurcation process leaves an unmistakable fingerprint in the form of newly‐described “muscle bridges.” These structures theoretically allow one to distinguish original and derived branches at each bifurcation. Last, we characterize some of the internal anatomical features of the stolons (reproductive units) of R. multicaudata, particularly their nervous system. Here, we provide the first study of the internal anatomy of a branched annelid. This information is not only crucial to deepen our understanding of these animals and their biology, but it will also be key to inform future studies that try to explain how this morphology evolved.

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Section: Synchrotron Radiation Micro-computed Tomographymentioning

confidence: 99%

Section: Synchrotron Radiation Micro-computed Tomographymentioning

confidence: 99%

Integrative anatomical study of the branched annelid Ramisyllis multicaudata (Annelida, Syllidae)

Ponz‐Segrelles

Glasby²,

Helm

et al. 2021

Journal of Morphology

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“…Three-dimensional models of both fossils were developed from SRµCT scans that were done at PETRA III in beamline P05 (operated by Helmholtz-Zentrum Hereon) at the Deutsches Elektronen Synchrotron DESY (Greving et al 2014;Haibel et al 2010) using a custom-developed 20 MP CMOS camera system (Lytaev et al 2014). Amber pieces were mounted on sample stubs with beeswax and then rotated through the beam, with each scan roughly taking 20-50 min.…”

Section: Methodsmentioning

confidence: 99%

The first fossils of the most basal pseudoscorpion family (Arachnida: Pseudoscorpiones: Pseudotyrannochthoniidae): evidence for major biogeographical shifts in the European paleofauna

Schwarze

Harms

Hammel

et al. 2021

PalZ

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Pseudoscorpions belong to the oldest terrestrial lineages with origins in the Devonian (ca. 385 Ma) but their fossil record is extremely sparse and little is known about their diversification over time. Here, we describe the first fossil species of the pseudoscorpion family Pseudotyrannochthoniidae that resemble the Devonian fossils in major details, such as the chaetotaxy of the pedipalps. We describe two new species, Allochthonius balticus sp. nov. from Baltic amber and Centrochthonius bitterfeldicus sp. nov. from Bitterfeld amber in northern Europe. Both species can unequivocally be assigned to extant genera and provide further evidence for dramatic range shifts in European invertebrate biota since the Paleogene. Allochthonius Chamberlin, 1929 is a diverse genus in eastern Asia (China, Korea, and Japan) today but does not occur anywhere in central Asia, Europe or North America. Centrochthonius Beier, 1931 is a poorly known genus but seems to be restricted to high altitude habitats in central Asia (China, Kyrgyzstan, and Nepal). With range retractions to regions more than 4600 km away from the European amber deposits, the fossils highlight total lineage extinction in Europe and survival in refugia that are climatically and botanically most similar to the Baltic amber forest of the Eocene. Overall, our results support the concept of morphological but potentially also ecological stasis in major pseudoscorpion lineages over long periods of time and agree with previous studies that suggested a warm temperate rather than subtropical or tropical climate for the time of amber deposition.

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“…This allowed for a high spatial resolution, high tissue contrast and rapid image acquisition. 177 specimens were scanned at the imaging cluster of the KIT Light Source (Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany), 39 specimens at the IBL-P05 imaging beamline [ 40 – 42 ] (operated by the Helmholtz-Zentrum Hereon at PETRA III, Deutsches Elektronen Synchrotron (DESY), Hamburg, Germany), and one specimen at the TOMCAT beamline [ 43 ] (Swiss Light Source (SLS), Paul-Scherrer-Institute (PSI), Villigen, Switzerland). Two larger specimens were scanned with a commercially available µCT-machine (phoenix nanotom, General Electric, Boston, MA) operated by Hereon.…”

Section: Methodsmentioning

confidence: 99%

Juvenile ecology drives adult morphology in two insect orders

et al. 2021

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Most animals undergo ecological niche shifts between distinct life phases, but such shifts can result in adaptive conflicts of phenotypic traits. Metamorphosis can reduce these conflicts by breaking up trait correlations, allowing each life phase to independently adapt to its ecological niche. This process is called adaptive decoupling. It is, however, yet unknown to what extent adaptive decoupling is realized on a macroevolutionary scale in hemimetabolous insects and if the degree of adaptive decoupling is correlated with the strength of ontogenetic niche shifts. It is also unclear whether the degree of adaptive decoupling is correlated with phenotypic disparity. Here, we quantify nymphal and adult trait correlations in 219 species across the whole phylogeny of earwigs and stoneflies to test whether juvenile and adult traits are decoupled from each other. We demonstrate that adult head morphology is largely driven by nymphal ecology, and that adult head shape disparity has increased with stronger ontogenetic niche shifts in some stonefly lineages. Our findings implicate that the hemimetabolan metamorphosis in earwigs and stoneflies does not allow for high degrees of adaptive decoupling, and that high phenotypic disparity can even be realized when the evolution of distinct life phases is coupled.

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Characterization of the CCD and CMOS cameras for grating-based phase-contrast tomography

Cited by 18 publications

References 10 publications

Integrative anatomical study of the branched annelid Ramisyllis multicaudata (Annelida, Syllidae)

Integrative anatomical study of the branched annelid Ramisyllis multicaudata (Annelida, Syllidae)

The first fossils of the most basal pseudoscorpion family (Arachnida: Pseudoscorpiones: Pseudotyrannochthoniidae): evidence for major biogeographical shifts in the European paleofauna

Juvenile ecology drives adult morphology in two insect orders

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