Characterization of the cardiac ganglion in the crab Neohelice granulata and immunohistochemical evidence of GABA-like extrinsic regulation

Abstract

Please cite this article as: Yang,, M., Carbó Tano,, M., Freudenthal,, R., Hermitte,, G., Characterization of the cardiac ganglion in the crab Neohelice granulata and immunohistochemical evidence of GABAlike extrinsic regulation, Arthropod Structure and Development (2012), doi: 10.1016/j.asd.2012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACTThe aim of the present work is to provide an anatomical description of the cardiac system in the crab Neohelice granulata and evidence of the presence of GABA by means of immunohistochemistry. The ganglionic trunk was found lying on the inner surface of the heart's dorsal wall. After dissection, this structure appeared as a Y-shaped figure with its major axis perpendicular to the major axis of the heart. Inside the cardiac ganglion, we identified four large neurons of 63.7 µm ± 3.7 in maximum diameter, which were similar to the motor neurons described in other decapods. All the GABA-like immunoreactivity (GABAi) was observed as processes entering mainly the ganglionic trunk and branching in slender varicose fibers, forming a network around the large neurons suggesting that GABAi processes contact them.Our findings strengthen previous results suggesting that the GABAergic system mediates the cardioinhibitory response upon sensory stimulation.

Picrotoxin but not bicuculline partially abolishes the cardio-inhibitory responses induced by visual stimulation in the crab Neohelice granulata

“…In our previous work in the crab Neohelice, immunohistochemical techniques have allowed us to identify GABA-like immunoreactive processes entering the heart, which later branched in slender and varicose fibers forming a network around the somas of large neurons of the cardiac ganglion. These results strengthen the idea that GABA has a role in the peripheral inhibition of the cardiac system [59].…”

Localization of chemical synapses and modulatory release sites in the cardiac ganglion of the crab, Cancer borealis

“…Although the CG contains just nine neurons, its stereotyped neuronal output is both flexible and robust (Cooke, 2002; Cruz-Bermúdez & Marder, 2007; Lane et al, 2016; Tazaki & Cooke, 1979). The physiological response of the crustacean cardiac ganglion to various neuromodulators has been well characterized in a variety of species (Cruz-Bermúdez & Marder, 2007; Dickinson et al, 2015; Lane et al, 2018; Yang et al, 2013). In addition to hormonal modulation, the CG is modulated extrinsically by at least three pairs of input nerves (Alexandrowicz, 1934).…”

“…Immunohistochemistry studies have visualized some modulatory inputs in whole-mount preparations of CGs in various species. Extensive GABA-like projections are found throughout the CG of the lobster P. argus (Delgado et al, 2000; Yang et al, 2013), and nitrous oxide synthase-like immunoreactivity is found in posterior motor neurons and small pacemaker cells of the crab C. productus (Scholz et al, 2002). Recent work has used MALDI imaging to characterize the distribution of neuropeptides in the cardiac ganglion of C. borealis , and confirmed 316 unique neuropeptides including those studied here (DeLaney & Li, 2020).…”

Localization of chemical synapses and modulatory release sites in the cardiac ganglion of the crab, Cancer borealis

“…Although the CG contains just nine neurons, its stereotyped neuronal output is both flexible and robust ( Cooke, 2002 ; Cruz-Bermúdez & Marder, 2007 ; Lane et al, 2016 ; Tazaki & Cooke, 1979 ). The physiological response of the crustacean CG to various neuromodulators has been characterized in a variety of species ( Cruz-Bermúdez & Marder, 2007 ; Dickinson et al, 2015 ; Lane et al, 2018 ; Yang et al, 2013 ). In addition to hormonal modulation, the CG is modulated extrinsically by at least three pairs of input nerves ( Alexandrowicz, 1934 ).…”

“…Immunohistochemistry studies have visualized some modulatory inputs in whole-mount preparations of CGs in various species. Extensive GABA-like projections are found throughout the CG of the lobster Panularis argus ( Delgado et al, 2000 ; Yang et al, 2013 ), and nitric oxide synthase-like immunoreactivity is found in posterior LC and small pacemaker cells of the crab Cancer productus ( Scholz et al, 2002 ). Recent work has used MALDI imaging to characterize the distribution of neuropeptides in Cancer borealis and confirmed 316 unique neuropeptides including those studied here ( DeLaney & Li, 2020 ).…”