Characterization of the bZip-Type Transcription Factor NapA with Reference to Oxidative Stress Response in<i>Aspergillus nidulans</i>

Asano, Yoshihiro; Hagiwara, Daisuke; Yamashino, Takafumi; Mizuno, Takeshi

doi:10.1271/bbb.70133

Cited by 53 publications

(51 citation statements)

References 9 publications

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“…Under the same growth conditions, the inoculated ÁnapA cells did not grow, or showed very poor growth, on the stress-inducing media, as reported previously. 17) To confirm these results at the molecular level, H 2 O 2 -dependent induction of stress responsive genes during hyphal growth was examined for ÁatfA and ÁnapA (Fig. 2B, catB encoding a catalase and trxB encoding a thioredoxin reductase).…”

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confidence: 80%

“…17) When we compared the amino acid sequences of AtfA and NapA, the sequences of the bZip domains were significantly similar (26% identical). Otherwise, their entire amino acid sequences and structural designs were quite different (Fig.…”

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confidence: 96%

“…1A). 17) We think that characterization of an Atf1 orthologous gene of A. nidulans, if present, is a timely issue to be addressed for a better understanding of the mechanisms underlying stress responses in this filamentous fungus, the life cycle of which is quite distinct from that of unicellular fission yeast. In this study, we identified an Atf1 homolog (designated AtfA) in A. nidulans, and we found that AtfA plays a prominent role in stress responsive transcriptional regulation, specifically in the conidia.…”

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confidence: 99%

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Characterization of bZip-Type Transcription Factor AtfA with Reference to Stress Responses of Conidia ofAspergillus nidulans

Hagiwara

Asano

Yamashino

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Characterization of bZip-Type Transcription Factor AtfA with Reference to Stress Responses of Conidia ofAspergillus nidulans

Hagiwara

Asano

Yamashino

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…As stated above, the mutation in cyp51A is most likely responsible for azole resistance. The afyap1 gene encodes an oxidative stress-responsive transcriptional regulator highly conserved among fungi (44,45). The AfYap1 protein has been reported to play a crucial role in oxidative stress tolerance but not in virulence (46).…”

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confidence: 99%

Whole-Genome Comparison of Aspergillus fumigatus Strains Serially Isolated from Patients with Aspergillosis

et al. 2014

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The emergence of azole-resistant strains of Aspergillus fumigatus during treatment for aspergillosis occurs by a mutation selection process. Understanding how antifungal resistance mechanisms evolve in the host environment during infection is of great clinical importance and biological interest. Here, we used next-generation sequencing (NGS) to identify mutations that arose during infection by A. fumigatus strains sequentially isolated from two patients, one with invasive pulmonary aspergillosis (IPA) (five isolations) and the other with aspergilloma (three isolations). The serial isolates had identical microsatellite types, but their growth rates and conidia production levels were dissimilar. A whole-genome comparison showed that three of the five isolates from the IPA patient carried a mutation, while 22 mutations, including six nonsynonymous ones, were found among three isolates from the aspergilloma patient. One aspergilloma isolate carried the cyp51A mutation P216L, which is reported to confer azole resistance, and it displayed an MIC indicating resistance to itraconazole. This isolate harbored five other nonsynonymous mutations, some of which were found in the afyap1 and aldA genes. We further identified a large deletion in the aspergilloma isolate in a region containing 11 genes. This finding suggested the possibility that genomic deletions can occur during chronic infection with A. fumigatus. Overall, our results revealed dynamic alterations that occur in the A. fumigatus genome within its host during infection and treatment.

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“…Introduction of the wild-type yapA gene into the mutant restored H 2 O 2 tolerance of the germinating spores to wild-type levels, confirming a role for YapA signaling in spore adaptation to H 2 O 2 oxidative stress. In A. nidulans, both SakA, through the transcription factor AtfA, and YapA pathways have been shown to be important for conferring spore resistance to H 2 O 2 stress, as both atfA and napA (equivalent to yapA) mutants are sensitive to H 2 O 2 stress (58,64,65). For the SakA pathway, tolerance to oxidative stress is mediated through a spore-specific catalase, CatA, whose expression is regulated by AtfA.…”

Section: Discussionmentioning

confidence: 99%

Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae

Cartwright

Scott

2013

Eukaryot Cell

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One of the central regulators of oxidative stress in Saccharomyces cerevisiae is Yap1, a bZIP transcription factor of the AP-1 family. In unstressed cells, Yap1 is reduced and cytoplasmic, but in response to oxidative stress, it becomes oxidized and accumulates in the nucleus. To date, there have been no reports on the role of AP-1-like transcription factors in symbiotic fungi. An ortholog of Yap1, named YapA, was identified in the genome of the grass symbiont Epichloë festucae and shown to complement an S. cerevisiae ⌬yap1 mutant. Hyphae of the E. festucae ⌬yapA strain were sensitive to menadione and diamide but resistant to H 2 O 2 , KO 2 , and tert-butyl hydroperoxide (t-BOOH). In contrast, conidia of the ⌬yapA strain were very sensitive to H 2 O 2 and failed to germinate. Using a PcatA-eGFP degron-tagged reporter, YapA was shown to be required for expression of a spore-specific catalase gene, catA. Although YapA-EGFP localized to the nucleus in response to host reactive oxygen species during seedling infection, there was no difference in whole-plant and cellular phenotypes of plants infected with the ⌬yapA strain compared to the wild-type strain. Homologs of the S. cerevisiae and Schizosaccharomyces pombe redox-sensing proteins (Gpx3 and Tpx1, respectively) did not act as redox sensors for YapA in E. festucae. In response to oxidative stress, YapA-EGFP localized to the nuclei of E. festucae ⌬gpxC, ⌬tpxA, and ⌬gpxC ⌬tpxA cells to the same degree as that in wild-type cells. These results show that E. festucae has a robust system for countering oxidative stress in culture and in planta but that Gpx3-or Tpx1-like thiol peroxidases are dispensable for activation of YapA.

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Characterization of the bZip-Type Transcription Factor NapA with Reference to Oxidative Stress Response inAspergillus nidulans

Cited by 53 publications

References 9 publications

Characterization of bZip-Type Transcription Factor AtfA with Reference to Stress Responses of Conidia ofAspergillus nidulans

Characterization of bZip-Type Transcription Factor AtfA with Reference to Stress Responses of Conidia ofAspergillus nidulans

Whole-Genome Comparison of Aspergillus fumigatus Strains Serially Isolated from Patients with Aspergillosis

Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae

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