Characterization of the bradykinin receptor in the human nasal airway using the binding of [¹²⁵I]‐Hoe 140

Abstract

1 The aim of this study was to characterize the kinin receptor in the human nasal airway using [1251] 7 Challenge of allergic subjects with house dust mite antigen caused a significant elevation of the bradykinin concentration in nasal lavage fluid and a reduction in Amin. Hoe 140, 100 fg, prevented the antigen-induced reduction in Amin and also abolished the antigen-induced increase of bradykinin in nasal lavage fluid. 8 We conclude that there is a B2 bradykinin receptor in the human nasal airway which mediates nasal blockage and plasma extravasation induced by either bradykinin or antigen challenge. It is possible that Hoe 140 inhibits kallikrein in the human nasal airway as well as blocking the B2 receptor.

MAPK/NF-κB-dependent upregulation of kinin receptors mediates airway hyperreactivity: A new perspective for the treatment

“…These responses can be attenuated by the kinin B 2 receptor antagonist MEN16132 [7]. In the human nasal airway, bradykinin receptors mediate nasal blockage and plasma extravasation induced by either bradykinin or antigen challenge [35]. Allergic rhinitis subjects display significantly higher expression of kinin B 1 receptor mRNA than normal subjects, and nasal allergen challenge increases kinin B 1 receptor mRNA expression in allergic rhinitis subjects [36].…”

International Union of Pharmacology. XLV. Classification of the Kinin Receptor Family: from Molecular Mechanisms to Pathophysiological Consequences

“…In practical terms, this peptide exhibits a high selectivity toward the B 2 receptor, although its affinity for the human B 1 receptor is measurable (Table 2). Icatibant has been used extensively (about 700 publications indexed in Medline) to assess the role of kinins in various models of physiological adaptation or experimental pathology and occasionally in humans (Groves et al, 1995;Akbary et al, 1996;Dear et al, 1996;Gainer et al, 1998;Squire et al, 2000;Witherow et al, 2001;Hornig et al, 2003).…”

Bradykinin increases intracellular calcium levels in a human bronchial epithelial cell line via the B 2 receptor subtype

“…Kinin receptors in human nasal airway [39] and in human tracheal submucosal glands [24] have been characterized to belong to the B 2 type. No evidence for kinin B 1 receptors was reported in these studies.…”