Characterization of the bla
            KPC-2 and bla
            KPC-3 genes and the novel bla
            KPC-15 gene in Klebsiella pneumoniae

Wang, Dongguo; Hou, Wei; Chen, Jiayu; Mou, Yi; Yang, Linjun; Yang, Libin; Sun, Xiulian

doi:10.1099/jmm.0.073841-0

Cited by 11 publications

(16 citation statements)

References 25 publications

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“…The transformant was also much less resistant than the original isolate, it was most likely due to the lack of any permeability lesion. The genetic environment of the bla KPC-15 gene in the plasmid showed a structural and context similarity to those characteristics found in plasmid pKP048 [33], except that a truncated bla TEM-12 gene and the bla KPC-15 variant were inserted and located between IS Kpn8 and the novel bla KPC-15 variant [24]. The nucleotides upstream of the bla KPC-15 translational start codon were also shown in Figure 3.…”

Section: Discussionmentioning

confidence: 65%

“…There were 5 different genes in the sequence, including the genes tnpR , bla TEM-12 and bla KPC-15 , and the transposons IS Kpn8 and IS Kpn6 -like [24]. The sites of the transcriptional promoters for the bla TEM-12 and bla KPC-15 genes were indicated as +1 and the −10 and −35 regions were also shown; however, there were no obvious transcriptional promoters in tnpR , IS Kpn8 and IS Kpn6 -like transposons.…”

Section: Resultsmentioning

confidence: 99%

“…The bla KPC-15 variant is on a ca. 73-kb plasmid and carries I SKpn8 and an IS Kpn6 -like element determinant [24]. The transformant was also much less resistant than the original isolate, it was most likely due to the lack of any permeability lesion.…”

Section: Discussionmentioning

confidence: 99%

“…In the study, we characterised phenotypic and enzymatic comparative analysis for the KPC-2 enzyme and novel KPC-15 variant which was discovered recently by us [24].…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Enzymatic Comparative Analysis of the KPC Variants, KPC-2 and Its Recently Discovered Variant KPC-15

et al. 2014

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Sixteen different variants (KPC-2 to KPC-17) in the KPC family have been reported, and most current studies are focusing on KPC-2 and KPC-3. The KPC-15 variant, which isolated from Klebsiella pneumoniae in a Chinese hospital, was a recently discovered KPC enzyme. To compare the characteristics of KPC-15 and KPC-2, the variants were determined by susceptibility testing, PCR amplification and sequencing, and study of kinetic parameters. The strain harboring the KPC-15 showed resistance to 18 conventional antimicrobial agents, especially to cabapenem antibiotics, and the strain involving the KPC-2 also indicated resistance to cabapenem antibiotics, but both strains were susceptible to polymyxin B and colistin. The conjugation experiments showed that the changes of MIC values to the antibiotics were due to the transferred plasmids. The differences of amino acids were characterised at sites of 119 leucine and 146 lysine with KPC-15 and KPC-2. The minimum evolution tree indicated the KPC alleles evolution, and showed that the KPC-15 appeared to be homogenous with KPC-4 closely. Steady-state kinetic parameters showed the catalytic efficiency of KPC-15 was higher than that of KPC-2 for all tested antibiotics in this study. The catalytic efficiency of KPC-15 caused resistance to β-lactam antibiotics was higher than that of KPC-2. Meanwhile, an evolutionary transformation changed KPC from an efficient carbapenemase to its variants (KPC-15) with better ceftazidimase catalytic efficiency, and the old antibiotics polymyxin B and colistin might play a role in the therapy for multi-resistant strains.

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Section: Discussionmentioning

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…In the study, we characterised phenotypic and enzymatic comparative analysis for the KPC-2 enzyme and novel KPC-15 variant which was discovered recently by us [24].…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Enzymatic Comparative Analysis of the KPC Variants, KPC-2 and Its Recently Discovered Variant KPC-15

et al. 2014

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“…These enzymes hydrolyze a wide spectrum of β-lactams, including penicillins, cephalosporins, and carbapenems [3]. The bla KPC gene possesses different variants (KPC-2 to KPC-15) that are sporadically found in other species, such as non-fermenting bacteria [4–7]. …”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular detection of the blaKPC gene in clinical isolates from inpatients at hospitals in São Luis, MA, Brazil

et al. 2016

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BackgroundBacteria that produce Klebsiella pneumoniae carbapenemases (KPCs) are resistant to broad-spectrum β-lactam antibiotics. The objective of this study was to phenotypically and genotypically characterize the antibiotic susceptibility to carbapenems of 297 isolates recovered from clinical samples obtained from inpatients at 16 hospitals in São Luis (Maranhão, Brazil).MethodsThe study was conducted using phenotypic tests and molecular methods, including polymerase chain reaction (PCR), sequencing and enterobacterial repetitive intergenic consensus (ERIC)-PCR. The nonparametric chi-square test of independence was used to evaluate the associations between the bacterial bla KPC gene and the modified Hodge test, and the chi-square adherence test was used to assess the frequency of carbapenemases and their association with the bla KPC gene.ResultsThe most frequently isolated species were Acinetobacter baumannii (n = 128; 43.0%), K. pneumoniae (n = 75; 25.2%), and Pseudomonas aeruginosa (n = 42; 14.1%). Susceptibility assays showed that polymixin B was active against 89.3% of the bacterial isolates. The Acinetobacter spp. and K. pneumoniae strains were susceptible to amikacin and tigecycline, and Pseudomonas spp. were sensitive to gentamicin and amikacin. Among the 297 isolates, 100 (33.7%) were positive for the bla KPC gene, including non-fermentative bacteria (A. baumannii) and Enterobacteriaceae species. Among the isolates positive for the bla KPC gene, K. pneumoniae isolates had the highest positivity rate of 60.0%. The bla KPC gene variants detected included KPC-2, which was found in all isolates belonging to species of the Enterobacteriaceae family. KPC-2 and KPC-3 were observed in A. baumannii isolates. Importantly, the bla KPC gene was also detected in three Raoultella isolates and one isolate of the Pantoea genus. ERIC-PCR patterns showed a high level of genetic diversity among the bacterial isolates; it was capable of distinguishing 34 clones among 100 strains that were positive for bla KPC and were circulating in 11 of the surveyed hospitals.ConclusionsThe high frequency of the bla KPC gene and the high degree of clonal diversity among microorganisms isolated from patients from different hospitals in São Luis suggest the need to improve the quality of health care to reduce the incidence of infections and the emergence of carbapenem resistance in these bacteria as well as other Gram-negative pathogens.

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Detection of clinical Serratia marcescens isolates carrying blaKPC-2 in a hospital in China

Tang,

Zhao,

et al. 2024

Heliyon

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Characterization of the bla KPC-2 and bla KPC-3 genes and the novel bla KPC-15 gene in Klebsiella pneumoniae

Cited by 11 publications

References 25 publications

Phenotypic and Enzymatic Comparative Analysis of the KPC Variants, KPC-2 and Its Recently Discovered Variant KPC-15

Phenotypic and Enzymatic Comparative Analysis of the KPC Variants, KPC-2 and Its Recently Discovered Variant KPC-15

Phenotypic and molecular detection of the blaKPC gene in clinical isolates from inpatients at hospitals in São Luis, MA, Brazil

Detection of clinical Serratia marcescens isolates carrying blaKPC-2 in a hospital in China

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