Characterization of the Binding of the RNA-Binding Protein AUF1 to the Human AT1 Receptor mRNA

Pende, Aldo; Giacchè, Mara; Castigliola, Lara; Contini, Lidia; Passerone, Giancarlo; Patrone, Mauro; Port, J. David; Lotti, G

doi:10.1006/bbrc.1999.1862

Cited by 21 publications

(23 citation statements)

References 23 publications

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“…In conclusion, the efficiency of ARE-dependent mRNA turnover is compromised in cells containing low levels of endogenous p37 AUF1 and p40 AUF1 (Ross et al, 1991;Buzby et al, 1996) or following sequestration of AUF1 by treatment with hemin (Loflin et al, 1999). Conversely, in congestive heart failure, an increase in AUF1 protein levels accompanies a lower expression of b 1 -adrenergic receptor mRNA, possibly mediated by an ARE-related mechanism (Pende et al, 1996(Pende et al, ,1999.…”

Section: Auf1mentioning

confidence: 99%

Post‐transcriptional regulation of gene expression by degradation of messenger RNAs

Bevilacqua

Ceriani

Capaccioli

et al. 2003

Journal Cellular Physiology

290

259

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Recent evidence suggests that gene expression may be regulated, at least in part, at post-transcriptional level by factors inducing the extremely rapid degradation of messenger RNAs. These factors include reactions between adenyl-uridyl-rich elements (AREs) of the relevant mRNA and either specific proteins that bind to these elements or exosomes. This review deals with examples of the proteins (AU-rich binding proteins, AUBPs) and exosomes, which have been shown to form complexes with AREs and bring about rapid degradation of the relevant mRNA, and with certain other factors, which protect the RNA from such degradation. The biochemical and physiological factors underlying the stability of messenger RNAs carrying the ARE motifs will be reviewed in the light of their emerging significance for cell physiology, human pathology, and molecular medicine. We also consider the possible application of the results of recent insights into the mechanisms to pharmacological interventions to prevent or cure disorders, especially developmental disorders, which the suppression of gene expression may bring about. Molecular targeting of specific steps in protein degradation by synthetic compounds has already been utilized for the development of pharmacological therapies.

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Section: Auf1mentioning

confidence: 99%

Post‐transcriptional regulation of gene expression by degradation of messenger RNAs

Bevilacqua

Ceriani

Capaccioli

et al. 2003

Journal Cellular Physiology

290

259

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“…37 Some have found an increase in mRNA levels associated with the C allele of rs5186. 38 On the contrary, Abdollahi et al 39 found rs5186 AGTR1 mRNA levels to be 0.8-fold lower in heterozygotes than major allele homozygotes, and 0.27-fold lower in minor allele homozygotes.…”

Section: Gene Variants and Frequencymentioning

confidence: 99%

Angiotensin II type 1 receptor polymorphisms and susceptibility to hypertension: A HuGE review

Mottl

Shoham

North

2008

Genetics in Medicine

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The angiotensin II type 1 receptor (AGTR1) plays an integral role in blood pressure control, and is implicated in the pathogenesis of hypertension. Polymorphisms within this gene have been extensively studied in association with hypertension; however, findings are conflicting. To clarify these data, we conducted a systematic review of association studies of AGTR1 polymorphisms and hypertension, and performed a meta-analysis of the rs5186 variant. Results show that the currently available literature is too heterogeneous to draw meaningful conclusions.The definition of hypertension and gender composition of individual studies helps to explain this heterogeneity.Although the structure and splicing pattern of AGTR1 would suggest a likely effect of polymorphisms within the promoter region on gene function, few studies have been conducted thus far. In conclusion, there is insufficient evidence that polymorphisms in the AGTR1 gene are risk factors for hypertension. However, most studies are inadequately powered, and larger well-designed studies of haplotypes are warranted. Genet Med 2008:10 (8): 560 -574.

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“…9 Briefly, radiolabeled RNAs were synthesized using the appropriate RNA polymerase and [a-32 P]UTP (800 Ci-mmol, Amersham, Milan, Italy) to produce uniformly labeled, 5'-capped RNAs. Transcripts were then treated with RNase-free DNase I to remove template DNA, extracted with phenol-chlorophorm and chlorophorm only, precipitated with ethanol, washed with 70% ethanol, and resuspended in RNase-free H 2 0.…”

Section: In Vitro Transcription Of Rna For Uv Cross-linkingmentioning

confidence: 99%

“…7 Previously, we demonstrated that purified recombinant p37 AUF1 protein binds to the human AT 1 (hAT 1 ) receptor mRNA. 9 Further, we showed that the RNA-binding protein AUF1 was able to bind to the distal portion of the 3'-untranslated region (3'-UTR), as well as to the coding region. Lastly we demonstrated that AUF1 protein is expressed endogenously in human VSMCs.…”

Section: Introductionmentioning

confidence: 99%

“…Lastly we demonstrated that AUF1 protein is expressed endogenously in human VSMCs. 9 In rat VSMCs Nickenig et al investigated the putative role of RNA-binding proteins, demonstrating that certain polysomal proteins, with an approximate Mr of 22 to 100 kDa, interacted with the distal portion of the 3'-UTR (bases 2175-2195). 10 Subsequent analysis determined that one of these mRNA binding proteins was calreticulin.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of RNA‐binding proteins possibly involved in modulating human AT₁ receptor mRNA stability

Pende

Contini

Sallo

et al. 2008

Cell Biochemistry & Function

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Angiotensin II exerts its cardiovascular effects mainly through the activation of AT(1) receptors. These receptors can be regulated at a post-transcriptional level, that is through the modulation of the mRNA stability. This regulation usually involves proteins which are able to bind the 3'UTR of the mRNA molecule. The experiments of the present paper were performed in order to characterize the RNA-binding proteins interacting with the hAT(1) receptor mRNA in human vascular smooth muscle cells. Immunoblot analysis allowed us to identify three different RNA-binding proteins, AUF1, HuR, and hnRNP A1. UV cross-linking and immunoprecipitation experiments demonstrated that AUF1 binds to the hAT(1)-receptor mRNA radiolabeled probes specifically, but in different ways in relation to the clinically important A/C gene polymorphism. Gel shift experiments using purified recombinant proteins confirmed the specificity of interaction of these proteins with the hAT(1)-receptor mRNA. In basal conditions the proteins were mainly located in the nuclei, but angiotensin II administration clearly induced their translocation to the cytosol. This observation was confirmed by transfection experiments using both GFP/AUF1 and GFP/HuR fusion proteins. Our findings allow identification of specific RNA-binding proteins possibly involved in the control of the hAT1-receptor mRNA stability and in the regulation of their expressions.

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Characterization of the Binding of the RNA-Binding Protein AUF1 to the Human AT1 Receptor mRNA

Cited by 21 publications

References 23 publications

Post‐transcriptional regulation of gene expression by degradation of messenger RNAs

Post‐transcriptional regulation of gene expression by degradation of messenger RNAs

Angiotensin II type 1 receptor polymorphisms and susceptibility to hypertension: A HuGE review

Characterization of RNA‐binding proteins possibly involved in modulating human AT₁ receptor mRNA stability

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Characterization of the Binding of the RNA-Binding Protein AUF1 to the Human AT1 Receptor mRNA

Cited by 21 publications

References 23 publications

Post‐transcriptional regulation of gene expression by degradation of messenger RNAs

Post‐transcriptional regulation of gene expression by degradation of messenger RNAs

Angiotensin II type 1 receptor polymorphisms and susceptibility to hypertension: A HuGE review

Characterization of RNA‐binding proteins possibly involved in modulating human AT1 receptor mRNA stability

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Characterization of RNA‐binding proteins possibly involved in modulating human AT₁ receptor mRNA stability