Characterization of the Bacteriophage vB_EfaS-271 Infecting Enterococcus faecalis

Topka-Bielecka, Gracja; Bloch, Sylwia; Nejman-Faleńczyk, Bożena; Grabski, Michał; Jurczak-Kurek, Agata; Górniak, Marcin; Dydecka, Aleksandra; Necel, Agnieszka; Węgrzyn, Grzegorz; Węgrzyn, Alicja

doi:10.3390/ijms21176345

Cited by 16 publications

(21 citation statements)

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“…Since development of phage therapy against E. faecalis appears to be important to establish effective treatments of infections by antibiotic-resistant strains of this bacterium [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ], and biofilm formation on catheters is one of the most difficult medical challenges caused by this species [ 13 ], we aimed to test efficiency of recently isolated and described bacteriophage vB_EfaS-271 [ 26 ] in killing E. faecalis cells included in biofilms formed on Foley silicone catheters. We found that vB_EfaS-271 is able to efficiently reduce number of viable bacterial cells under such conditions ( Figure 1 ), providing evidence for its possible use in medical practice to either prevent colonization of catheters by E. faecalis or eradication of this bacterium from already formed biofilms in these medical devices.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteriophage vB_EfaS-271 was isolated from urban sewage (Gdansk Wastewater Treatment Plant, Poland) and deposited in the collection of phage strains of the Department of Molecular Biology of the University of Gdansk [ 26 , 28 ]. To obtain purified vB_EfaS-271 particles, E. faecalis 271 host was cultivated in the liquid TSB medium at 37 °C to an OD 600 of 0.3.…”

Section: Methodsmentioning

confidence: 99%

“…In fact, biodiversity of phages specific to bacteria belonging to this species is relatively high, making it possible to obtain a collection containing effective antibacterial agents to treat infections caused by different E. faecalis strains [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. In our previous work, we have isolated and characterized an E. faecalis -specific bacteriophage vB_EfaS-271 [ 26 ]. This phage has been classified as a member of Siphoviridae family, having a dsDNA genome of about 40 kb.…”

Section: Introductionmentioning

confidence: 99%

“…This phage has been classified as a member of Siphoviridae family, having a dsDNA genome of about 40 kb. It is a virulent phage, revealing a short (8 min) latent period and average burst size of 70 phages per cell under optimal laboratory conditions (37 °C, rich bacteriological medium) [ 26 ]. In this work, we aimed to characterize vB_EfaS-271-host interactions in the light of potential application of this virus in phage therapy, and to test toxicity of vB_EfaS-271 to mammalian cells in assessment of its safety to treat patients.…”

Section: Introductionmentioning

confidence: 99%

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Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis

Topka-Bielecka

Nejman-Faleńczyk

Bloch

et al. 2021

Viruses

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Phage therapy is one of main alternative option for antibiotic treatment of bacterial infections, particularly in the era of appearance of pathogenic strains revealing resistance to most or even all known antibiotics. Enterococcus faecalis is one of such pathogens causing serious human infections. In the light of high level of biodiversity of bacteriophages and specificity of phages to bacterial species or even strains, development of effective phage therapy depend, between others, on identification and characterization of a large collection of these viruses, including understanding of their interactions with host bacterial cells. Recently, isolation of molecular characterization of bacteriophage vB_EfaS-271, infecting E. faecalis strains have been reported. In this report, phage–host interactions are reported, including ability of vB_EfaS-271 to infect bacteria forming biofilms, efficiency of eliminating bacterial cells from cultures depending on multiplicity of infection (m.o.i.), toxicity of purified phage particles to mammalian cells, and efficiency of appearance of phage-resistant bacteria. The presented results indicate that vB_EfaS-271 can significantly decrease number of viable E. faecalis cells in biofilms and in liquid cultures and reveals no considerable toxicity to mammalian cells. Efficiency of formation of phage-resistant bacteria was dependent on m.o.i. and was higher when the virion-cell ratio was as high as 10 than at low (between 0.01 and 0.0001) m.o.i. values. We conclude that vB_EfaS-271 may be considered as a candidate for its further use in phage therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis

Topka-Bielecka

Nejman-Faleńczyk

Bloch

et al. 2021

Viruses

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“…The mass spectrometry data is crucial as it enables a discrimination between false positive (FP; predicted but not present by mass spectrometry of the virion) and true positive (TP; predicted and found present by mass spectrometry of the virion). Phage vB_EfaS_271 infects Enterococcus faecalis (26), phage vB_PatM_CB7 infects Pectobacterium atrosepticum (27), and phage vB_Eco4M-7 infects enteropathogenic Escherichia coli (28). STEP 3 was benchmarked against equivalent predictors: PVPred, PVP-SVM, PVPred-SCM and Pred-BVP-Unb (Fig.…”

Section: Resultsmentioning

confidence: 99%

The component parts of bacteriophage virions accurately defined by a machine-learning approach built on evolutionary features

Thung

White

Dai

et al. 2021

Preprint

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Antimicrobial resistance (AMR) continues to evolve as a major threat to human health and new strategies are required for the treatment of AMR infections. Bacteriophages (phages) that kill bacterial pathogens are being identified for use in phage therapies, with the intention to apply these bactericidal viruses directly into the infection sites in bespoke phage cocktails. Despite the great unsampled phage diversity for this purpose, an issue hampering the roll out of phage therapy is the poor quality annotation of many of the phage genomes, particularly for those from infrequently sampled environmental sources. We developed a computational tool called STEP3 to use the “evolutionary features” that can be recognized in genome sequences of diverse phages. These features, when integrated into an ensemble framework, achieved a stable and robust prediction performance when benchmarked against other prediction tools using phages from diverse sources. Validation of the prediction accuracy of STEP3 was conducted with high-resolution mass spectrometry analysis of two novel phages, isolated from a watercourse in the Southern Hemisphere. STEP3 provides a robust computational approach to distinguish specific and universal features in phages to improve the quality of phage cocktails, and is available for use at http://step3.erc.monash.edu/.IMPORTANCEIn response to the global problem of antimicrobial resistance there are moves to use bacteriophages (phages) as therapeutic agents. Selecting which phages will be effective therapeutics relies on interpreting features contributing to shelf-life and applicability to diagnosed infections. However, the protein components of the phage virions that dictate these properties vary so much in sequence that best estimates suggest failure to recognize up to 90% of them. We have utilised this diversity in evolutionary features as an advantage, to apply machine learning for prediction accuracy for diverse components in phage virions. We benchmark this new tool showing the accurate recognition and evaluation of phage components parts using genome sequence data of phages from under-sampled environments, where the richest diversity of phage still lies.

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Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy

Kosznik-Kwaśnicka,

Topka,

Mantej

et al. 2023

Methods in Molecular Biology

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Characterization of the Bacteriophage vB_EfaS-271 Infecting Enterococcus faecalis

Cited by 16 publications

References 99 publications

Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis

Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis

The component parts of bacteriophage virions accurately defined by a machine-learning approach built on evolutionary features

Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy

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