Characterization of the Asian Citrus Psyllid Transcriptome

Reese, Justin; Christenson, Matthew K.; Li, Nan; Saha, Surya; Cantarel, Brandi L.; Lindeberg, Magdalen; Tamborindeguy, Cécilia; MacCarthy, Justin; Weaver, Daniel; Trease, Andrew J.; Ready, Steven V.; Davis, Vincent M.; McCormick, Courtney; Haudenschild, Christian; Han, Shunsheng; Johnson, Shannon L.; Shelby, Kent S.; Huang, Hong; Bextine, Blake; Shatters, Robert G.; Hall, David G.; Davis, Paul H.; Hunter, Wayne B.

doi:10.7150/jgen.7692

Cited by 49 publications

(64 citation statements)

References 8 publications

(12 reference statements)

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“…RNA purification and sequencing followed identical protocols as described in Reese et al (2014). Total RNA was extracted using the Qiagen RNeasy Mini Kit, per manufacturer recommended protocol.…”

Section: Methodsmentioning

confidence: 99%

“…To overcome these immune barriers, insect pathogens have evolved mechanisms to avoid the insect defenses, such as not expressing PAMPs, degrading effectors, inhibiting immune signaling pathways, or killing hemocytes (Apidianakis et al, 2005; Vallet-Gely et al, 2008). Understanding vector immune responses may lead to novel management strategies to reduce insect-transmitted pathogens (Reese et al, 2014). This is evident in the recently successful manipulation of mosquito immune responses through the introduction of Wolbachia , resulting in the upregulation of immunity genes and inhibition of dengue virus replication (Moreira et al, 2009; Pan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System

2016

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Citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as Huanglongbing. The citrus greening bacteria, Candidatus Liberibacter asiaticus (CLas), is a persistent propagative pathogen transmitted by the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Hemipterans characterized to date lack a number of insect immune genes, including those associated with the Imd pathway targeting Gram-negative bacteria. The D. citri draft genome was used to characterize the immune defense genes present in D. citri. Predicted mRNAs identified by screening the published D. citri annotated draft genome were manually searched using a custom database of immune genes from previously annotated insect genomes. Toll and JAK/STAT pathways, general defense genes Dual oxidase, Nitric oxide synthase, prophenoloxidase, and cellular immune defense genes were present in D. citri. In contrast, D. citri lacked genes for the Imd pathway, most antimicrobial peptides, 1,3-β-glucan recognition proteins (GNBPs), and complete peptidoglycan recognition proteins. These data suggest that D. citri has a reduced immune capability similar to that observed in A. pisum, P. humanus, and R. prolixus. The absence of immune system genes from the D. citri genome may facilitate CLas infections, and is possibly compensated for by their relationship with their microbial endosymbionts.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System

2016

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“…Regarding the heavily‐studied C Las ‐ D. citri ‐citrus pathosystem, the whole genomes of C Las and sweet orange ( Citrus sinensis ) are available (Duan et al ., ; Xu et al ., ) and the transcriptomic, proteomic, biochemical and anatomic changes in citrus plant in response to C Las infection are also well documented (Albrecht & Bowman, , ; Fan et al ., , ; Nwugo et al ., ). For D. citri , several studies at the genetic and transcriptomic level are reported (Tiwari et al ., ; Killiny et al ., ; Reese et al ., ), although, to our knowledge, the comparative proteomic study of D. citri in response to C Las infection is not yet available. In proteomic studies, separating proteins by one‐ and two‐dimensional gel electrophoresis or liquid chromatography (LC), followed by mass spectrometry (MS), is generally considered as the standard method (Wu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Huanglongbing pathogen CandidatusLiberibacter asiaticus exploits the energy metabolism and host defence responses of its vector Diaphorina citri

Killiny

2017

Physiol. Entomol

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Asian citrus psyllid Diaphorina citri is the vector of the citrus Huanglongbing (HLB) associated bacterial agent ‘Candidatus Liberibacter asiaticus’ (CLas). The molecular interactions between CLas and D. citri remain unclear. In the present study, protein profiles of mitochondrial, microsomal and cytosolic fractions from uninfected and CLas‐infected adult D. citri are investigated using two‐dimensional gel electrophoresis. The comparative analysis reveals a total of 18, 24 and 20 protein spots that are unique or differentially expressed in mitochondrial, microsomal and cytosolic proteins fractions respectively. These proteins are successfully identified by mass spectrometry. Among the 62 identified proteins, 30 are up‐regulated, whereas 32 are down‐regulated. These proteins include important components in energy metabolism such as ATP synthase, ATPase, ATP/ADP carrier protein, etc.; host stress responses such as heat shock proteins; host detoxification processes (i.e., cytochrome P450 and glutathione S‐transferase); and the cytoskeleton (such as actin, tubulin, myosin and tropomyosin). These data suggest that, after CLas infection, several proteins of D. citri, especially energy metabolism and protein biosynthesis, are altered, and extensive host defence responses are induced. In conclusion, the present study reports proteomic information that is helpful in understanding the vector–pathogen relationship between CLas and D. citri, and could be used to identify potential targets for limiting the spread of CLas, as well as to provide new insights into HLB management.

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“…Several psyllid transcriptomic resources have been developed to identify psyllid genes differentially expressed in response to ‘ Candidatus Liberibacter spp.’ bacteria as well as to study psyllid physiology (Nachappa et al , ; Fisher et al , ; Reese et al , ). However, until now no study has been aimed at identifying a set of genes suitable to be used as reference genes in psyllid reverse transcription quantitative polymerase chain reaction (RT‐qPCR) analyses.…”

Section: Introductionmentioning

confidence: 99%

Selection of reference genes for expression analysis in the potato psyllid, Bactericera cockerelli

Ibáñez

Tamborindeguy

2016

Insect Molecular Biology

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The selection of reference genes is a crucial step for quantitative real-time PCR analyses and increasingly the use of more than one reference gene for accurate and reliable normalization is being recommended. In this study, a set of six genes was selected and their stability was assessed in different life stages and female organs of Bactericera cockerelli harbouring or not the bacterial pathogen 'Candidatus Liberibacter solanacearum' (Lso) haplotype B. The stability of each gene was determined using the BestKeeper, NormFinder and GeNorm programs. These analyses identified elongation factor-1a, ribosomal protein subunit L5 and ribosomal protein subunit 18 as the most stable genes to analyse gene expression during the insect life stages irrespective of Lso presence; Lso haplotype B only affected their respective ranking. By contrast, a common set of normalizers could not be found amongst the different female organs tested (bacteriomes, alimentary canals and reproductive organs).

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Characterization of the Asian Citrus Psyllid Transcriptome

Cited by 49 publications

References 8 publications

Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System

Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System

Huanglongbing pathogen CandidatusLiberibacter asiaticus exploits the energy metabolism and host defence responses of its vector Diaphorina citri

Selection of reference genes for expression analysis in the potato psyllid, Bactericera cockerelli

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