Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization

Robledo, Germán; Seijo, Guillermo

doi:10.1590/s1415-47572008000400019

Cited by 41 publications

(44 citation statements)

References 35 publications

(42 reference statements)

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“…PCR reactions were performed using divergent primers (A5Sfwd and A5Srev) designed on the transcribed region to amplify the whole repeating unit (Robledo and Seijo 2008). The reaction mixture was the same as that used for cpDNA regions.…”

Section: S Rdna Amplification and Sequencingmentioning

confidence: 99%

Genetic and geographic origin of domesticated peanut as evidenced by 5S rDNA and chloroplast DNA sequences

et al. 2012

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Section: S Rdna Amplification and Sequencingmentioning

confidence: 99%

Genetic and geographic origin of domesticated peanut as evidenced by 5S rDNA and chloroplast DNA sequences

et al. 2012

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“…The 5S rDNA and 18S-26S rDNA loci were localized using probes isolated from genomic DNA of Arachis hypogaea (Robledo and Seijo 2008). Probes were labelled by nick translation with digoxigenin-11-dUTP (Boehringer Mannheim, Mannheim, Germany) or biotin-11-dUTP (Sigma-Aldrich, St. Louis, Missouri, USA).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Structural karyotypic variability and polyploidy in natural populations of the South American Lathyrus nervosus Lam. (Fabaceae)

et al. 2012

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Knowledge of the chromosome variation in wild populations is essential to understand the pathways and restrictions of karyotype evolution in plants. The aim of this study is to conduct an intraspecific analysis of the karyotypes by fluorochrome banding and ribosomal DNA (rDNA) loci detection by fluorescent in situ hybridization (FISH) and of the meiotic behaviour in natural populations of Lathyrus nervosus, sect. Notolathyrus. Chromosome banding showed that, despite the high constancy in the karyotype formula and in the rDNA loci among populations, there is intraspecific variation in the amount and distribution pattern of 4',6-diamidino-2-phenylindole (DAPI ? ) heterochromatin. However, those changes were not related to the total chromosome length of the haploid complements. This fact demonstrates that structural chromosome changes may be one of the most important mechanisms for karyotype variation among natural populations of L. nervosus. The chromosome number surveyed at the population level revealed the first case of polyploidy in South American species and the first case of uneven polyploidy of the genus. All the chromosome markers analysed indicated that the polyploids found originated by autopolyploidy. The meiotic analysis showed different chromosome abnormalities that may be generating numerical and structural changes in the sporads. The finding of unreduced gametes that are alive at anthesis suggests sexual polyploidization as the most probable mechanism involved in the origin of these 3x and 4x autopolyploid cytotypes in L. nervosus.

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“…LTR of the FIDEL retrotransposon were amplified according to the method of Nielen et al [2010]. 5S and 18S rDNA were amplified according to Robledo and Seijo [2008] from A. hypogaea L. Purified PCR products were labeled with Alexa Fluor 594-5-dUTP (red) or Alexa Fluor 488-5-dUTP (green) (Invitrogen Life Technologies) by a modified version of the nick translation method described by Kato et al [2004].…”

Section: Dna Probe Preparationmentioning

confidence: 99%

Cloning and Characterization of Chromosomal Markers from a Cot-1 Library of Peanut (<b><i>Arachis hypogaea</i></b> L.)

Zhang

2012

Cytogenet Genome Res

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The cultivated peanut, Arachis hypogaea (AABB, 2n = 40), is an allotetraploid which was probably originated from a hybridization event between 2 ancestors, A. duranensis (A genome) and A. ipaensis (B genome) followed by chromosome doubling. The wild species in the Arachis section are useful genetic resources for genes that confer biotic and abiotic stress resistance for peanut breeding. However, the resource is not well exploited because little information on the genetic, cytogenetic, and phylogenetic relationships between cultivated peanut and its wild relatives is known. Characterization of its chromosome components will benefit the understanding of these issues. But the paucity of information on the DNA sequence and the presence of morphologically similar chromosomes impede the construction of a detailed karyotype for peanut chromosome identification. In our study, a peanut Cot-1 library was constructed to isolate highly and moderately repetitive sequences from the cultivated peanut, and the chromosomal distributions of these repeats were investigated. Both genome and chromosome specific markers were identified that allowed the distinguishing of A and B genomes in tetraploid peanut and a possible karyotyping of peanut chromosomes by FISH. In particular, a 115-bp tandem repetitive sequence was identified to be a possible centromere repetitive DNA, mainly localized in the centromeres of B chromosomes, and a partial retrotransposable element was also identified in the centromeres of B chromosomes. The cloning and characterization of various chromosomal markers is a major step for FISH-based karyotyping of peanut. The FISH markers are expected to provide a reference tool for sequence assembly, phylogenetic studies of peanut and its wild species, and breeding.

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Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization

Cited by 41 publications

References 35 publications

Genetic and geographic origin of domesticated peanut as evidenced by 5S rDNA and chloroplast DNA sequences

Genetic and geographic origin of domesticated peanut as evidenced by 5S rDNA and chloroplast DNA sequences

Structural karyotypic variability and polyploidy in natural populations of the South American Lathyrus nervosus Lam. (Fabaceae)

Cloning and Characterization of Chromosomal Markers from a Cot-1 Library of Peanut (<b><i>Arachis hypogaea</i></b> L.)

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