Characterization of the Apical Membrane Antigen-1 in Italian Strains of Babesia bigemina

Torina, Alessandra; Agnone, Annalisa; Sireci, Guido; Mosqueda, Juan; Blanda, Valeria; Albanese, Ida; Farina, Mário; Cerrone, Anna; Cusumano, F.; Caracappa, Santo

doi:10.1111/j.1865-1682.2010.01118.x

Cited by 20 publications

(17 citation statements)

References 19 publications

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“…In the phylogenetic analyses, the B. bovis RAP-1 and B. bigemina AMA-1 sequences were detected in single clade of their respective trees. However, the gene fragments analyzed in this study are relatively conserved among field isolates from different geographic locations [40,41]. Therefore, analyzing suitable marker genes, such as merozoite surface antigen genes in B. bovis, might provide a better picture of the genetic diversity in B.…”

Section: Discussionmentioning

confidence: 98%

An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt

Elsify

Sivakumar

Nayel

et al. 2015

Parasitology International

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Cattle, buffaloes, and sheep are the main sources of meat and milk in Egypt, but their productivity is thought to be greatly reduced by hemoprotozoan parasitic diseases. In this study, we analyzed the infection rates of Babesia bovis, Babesia bigemina, Theileria annulata, and Theileria orientalis, using parasite-specific PCR assays in blood-DNA samples sourced from cattle (n=439), buffaloes (n=50), and sheep (n=105) reared in Menoufia, Behera, Giza, and Sohag provinces of Egypt. In cattle, the positive rates of B. bovis, B. bigemina, T. annulata, and T. orientalis were 3.18%, 7.97%, 9.56%, and 0.68%, respectively. On the other hand, B. bovis and T. orientalis were the only parasites detected in buffaloes and each of these parasites was only found in two individual DNA samples (both 2%), while one (0.95%) and two (1.90%) of the sheep samples were positive for B. bovis and B. bigemina, respectively. Sequence analysis showed that the B. bovis Rhoptry Associated Protein-1 and the B. bigemina Apical Membrane Antigen-1 genes were highly conserved among the samples, with 99.3-100% and 95.3-100% sequence identity values, respectively. In contrast, the Egyptian T. annulata merozoite surface antigen-1 gene sequences were relatively diverse (87.8-100% identity values), dispersing themselves across several clades in the phylogenetic tree containing sequences from other countries. Additionally, the T. orientalis Major Piroplasm Surface Protein (MPSP) gene sequences were classified as types 1 and 2. This is the first report of T. orientalis in Egypt, and of type 2 MPSP in buffaloes. Detection of MPSP type 2, which is considered a relatively virulent genotype, suggests that T. orientalis infection may have veterinary and economic significance in Egypt. In conclusion, the present study, which analyzed multiple species of Babesia and Theileria parasites in different livestock animals, may shed an additional light on the epidemiology of hemoprotozoan parasites in Egypt.

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Section: Discussionmentioning

confidence: 98%

An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt

Elsify

Sivakumar

Nayel

et al. 2015

Parasitology International

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“…AMA-1 protein is conserved among apicomplexan parasites [ 12 ] and has been characterized in the cattle Babesia species B. bovis [ 13 ], B. bigemina [ 14 ], B. ovata [ 15 ] and B. divergens [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China

Niu

Liu

et al. 2015

Parasites Vectors

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BackgroundBabesiosis is an important haemoparasitic disease, caused by the infection and subsequent intra-erythrocytic multiplication of protozoa of the genus Babesia that impacts the livestock industry and animal health. The distribution, epidemiology and genetic characterization of B. bigemina, B. bovis, and B. ovata in cattle in China as well as the prevalence of these protozoan agents were assessed.MethodsA total of 646 blood specimens from cattle, dairy cattle and yaks from 14 provinces were collected and tested for the presence of the three Babesia species via a specific nested PCR assay based on the rap-1 and ama-1 genes. The PCR results were confirmed by DNA sequencing. Gene sequences and the genetic characterization were determined for selected positive samples from each sampling area.ResultsOf a total of 646 samples, 134 (20.7 %), 60 (9.3 %) and 10 (1.5 %) were positive for B. bovis, B. bigemina and B. ovata infections, respectively. Mixed infections were found in 7 of 14 provinces; 43 (6.7 %) samples were infected with B. bovis and B. bigemina. Three samples (0.5 %) exhibited a co-infection with B. bovis and B. ovata, and 6 (0.9 %) were infected with all three parasites. The rap-1a gene of B. bovis indicated a high degree of sequence heterogeneity compared with other published rap-1a sequences worldwide and was 85–100 % identical to B. bovis rap-1a sequences in Chinese isolates. B. bigemina rap-1c and B. ovata ama-1 genes were nearly identical, with 97.8–99.3 % and 97.8–99.6 % sequence identity, respectively, in GenBank.ConclusionsPositive rates of B. bovis and B. bigemina infection are somewhat high in China. The B. bovis infection in yaks was first reported. The significant sequence heterogeneity in different variants of the rap-1a gene from Chinese B. bovis isolates might be a great threat to the cattle industry if RAP-1a protein is used as immunological antigen against Babesia infections in China. The data obtained in this study can be used to plan effective control strategies against babesiosis in China.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-1110-0) contains supplementary material, which is available to authorized users.

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“…This last statement proves that the isolates analyzed are different because of their genotype, and among them, there is a highly conserved ama-1 gene [72]. The B. bigemina ama-1 seems to be conserved as well, and Italian strains have a conserved sequence among them and considerable differences in comparison with Australian reference strains; however, when these are compared with Mexican and Argentinian reference strains, the sequence matches in a 99% of identity [73]. The conservation level makes AMA-1 an excellent target for vaccine development.…”

Section: Ama-1mentioning

confidence: 95%

Genome-Based Vaccinology Applied to Bovine Babesiosis

Mosqueda¹,

Hernández-Silva²,

MarioHidalgo-Ruiz³

2018

Farm Animals Diseases, Recent Omic Trends and New Strategies of Treatment

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Genomics approaches in veterinary research have been a very useful tool to identify candidates with potential to be used in prevention of animal diseases. In Babesia, genome information analysis has elucidated a wide variety of protein families and some members are described in this chapter. Here, we present some of the most recent studies about B. bovis and B. bigemina genomes where some proteins have been identified with potential to prevent infections by these parasites.

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Characterization of the Apical Membrane Antigen-1 in Italian Strains of Babesia bigemina

Cited by 20 publications

References 19 publications

An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt

An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt

Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China

Genome-Based Vaccinology Applied to Bovine Babesiosis

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