Characterization of the APETALA2/Ethylene-responsive factor (AP2/ERF) transcription factor family in sunflower

Najafi, S. Iraj; Sorkheh, Karim; Nasernakhaei, Fatemeh

doi:10.1038/s41598-018-29526-z

Cited by 58 publications

(43 citation statements)

References 93 publications

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“…For example, APETALA2-like ethylene-responsive transcription factors (EVM0000677.1, EVM0023986.1, EVM0021041.1, and EVM0019895.1) were identified as being under divergent selection in PC1. This plant-specific transcription factor has been shown to play an important role in regulating plant growth and responses to cold, heat, salt, drought and flooding 39 , 40 . Peroxidase (EVM0032298.1), an enzyme that catalyzes the oxidation of a particular substrate by hydrogen peroxide, was differentially expressed under drought and low temperature stress 41 , 42 .…”

Section: Discussionmentioning

confidence: 99%

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Cushman

Yan

et al. 2020

Hortic Res

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Understanding the genetic basis underlying the local adaptation of nonmodel species is a fundamental goal in evolutionary biology. In this study, we explored the genetic mechanisms of the local adaptation of Forsythia suspensa using genome sequence and population genomics data obtained from specific-locus amplified fragment sequencing. We assembled a high-quality reference genome of weeping forsythia (Scaffold N50 = 7.3 Mb) using ultralong Nanopore reads. Then, genome-wide comparative analysis was performed for 15 natural populations of weeping forsythia across its current distribution range. Our results revealed that candidate genes associated with local adaptation are functionally correlated with solar radiation, temperature and water variables across heterogeneous environmental scenarios. In particular, solar radiation during the period of fruit development and seed drying after ripening, cold, and drought significantly contributed to the adaptive differentiation of F. suspensa. Natural selection exerted by environmental factors contributed substantially to the population genetic structure of F. suspensa. Our results supported the hypothesis that adaptive differentiation should be highly pronounced in the genes involved in signal crosstalk between different environmental variables. Our population genomics study of F. suspensa provides insights into the fundamental genetic mechanisms of the local adaptation of plant species to climatic gradients.

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Section: Discussionmentioning

confidence: 99%

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Cushman

Yan

et al. 2020

Hortic Res

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“…Interestingly, the TaMYB2 gene of wheat is used as a molecular marker for drought stress tolerance in common wheat [51]. The characterization of the HaAP2/ERF transcription-factor family showed that the AP2/ERF -family genes were overexpressed as a result of drought, cold, salt and heat treatments [52]. Additionally, the watermelon ClWRKYs had a diverse response to abiotic stresses, which suggests that WRKYs positively or negatively participate in plant tolerance of drought, salt or cold stress [53].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana

Patankar

Al-Harrasi

Kharusi

et al. 2019

IJMS

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Although the date palm tree is an extremophile with tolerance to drought and certain levels of salinity, the damage caused by extreme salt concentrations in the soil, has created a need to explore stress-responsive traits and decode their mechanisms. Metallothioneins (MTs) are low-molecular-weight cysteine-rich proteins that are known to play a role in decreasing oxidative damage during abiotic stress conditions. Our previous study identified date palm metallothionein 2A (PdMT2A) as a salt-responsive gene, which has been functionally characterized in yeast and Arabidopsis in this study. The recombinant PdMT2A protein produced in Escherichia coli showed high reactivity against the substrate 5′-dithiobis-2-nitrobenzoic acid (DTNB), implying that the protein has the property of scavenging reactive oxygen species (ROS). Heterologous overexpression of PdMT2A in yeast (Saccharomyces cerevisiae) conferred tolerance to drought, salinity and oxidative stresses. The PdMT2A gene was also overexpressed in Arabidopsis, to assess its stress protective function in planta. Compared to the wild-type control, the transgenic plants accumulated less Na+ and maintained a high K+/Na+ ratio, which could be attributed to the regulatory role of the transgene on transporters such as HKT, as demonstrated by qPCR assay. In addition, transgenic lines exhibited higher chlorophyll content, higher superoxide dismutase (SOD) activity and improved scavenging ability for reactive oxygen species (ROS), coupled with a better survival rate during salt stress conditions. Similarly, the transgenic plants also displayed better drought and oxidative stress tolerance. Collectively, both in vitro and in planta studies revealed a role for PdMT2A in salt, drought, and oxidative stress tolerance.

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“…Such a group of transcription factors in plants is activated by ethylene signaling under biotic or abiotic stresses. APETALA2/ethylene response factors (AP2/ERFs) are a superfamily of plant-exclusive transcription factors involved in the ethylene-inducible response (Gutterson and Reuber 2004 ; Mizoi et al 2012 ; Najafi et al 2018 ). APETALA2 was first isolated from the floral development-related proteins in Arabidopsis (Jofuku et al 1994 ).…”

Section: Introductionmentioning

confidence: 99%

Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

et al. 2020

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The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

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Characterization of the APETALA2/Ethylene-responsive factor (AP2/ERF) transcription factor family in sunflower

Cited by 58 publications

References 93 publications

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana

Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

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