Characterization of the antioxidant fraction of Trapa japonica pericarp and its hepatic protective effects in vitro and in vivo

Kim, Yon-Suk; Kim, Min Jung; Hwang, Jin-Woo; Seo, Il-bok; Jang, Jae‐Hyung; Son, Sangkeun; Jeong, Jae-Hyun; Moon, Sang-Ho; Jeon, Byong-Tae; Park, Pyo-Jam

doi:10.1039/c5fo01405k

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…In vivo models have indicated that Trapa japonica Flerov. significantly attenuated t-BHP-induced increases in serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase levels, and in hepatic malondialdehyde levels [ 48 ]. Guarana has also been used as a tonic for hangovers, neuralgia and menstrual headaches, leucorrhea, diarrhea, and fevers; this compound has been shown to prevent DNA damage in carbon tetrachloride-treated rats [ 18 20 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of DA-5513 on alcohol metabolism and alcoholic fatty liver in rats

Nguyen

Yong

et al. 2018

Lab Anim Res

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Hangover is characterized by a number of unpleasant physical and mental symptoms that occur after heavy alcohol drinking. In addition, consistently excessive alcohol intake is considered as a major reason causes liver disease. The present study investigated the in vivo effects of DA-5513 (Morning care® Kang Hwang) on biological parameters relevant to hangover relief and alcoholic fatty liver. Blood alcohol and acetaldehyde concentrations were determined in rats administered a single dose of alcohol and treated with DA-5513 or commercially available hangover relief beverages (Yeomyung® and Ukon®). The effects of DA-5513 on alcoholic fatty liver were also determined in rats fed alcohol-containing Lieber-DeCarli diets for 4 weeks. Serum liver function markers (aspartate and alanine aminotransferase activities) and serum/liver lipid levels were assessed. Blood alcohol and acetaldehyde concentrations were lower in the groups treated with DA-5513 or Yeomyung®, as compared with control rats. However, Ukon® did not produce any significant effects on these parameters. Treatment with DA-5513 significantly reduced serum aspartate and alanine aminotransferase activities and markedly reduced serum cholesterol and triglyceride levels, as compared with control rats. Histological observations using Oil Red O staining found that DA-5513 delayed the development of alcoholic fatty liver by reversing hepatic fat accumulation. These findings suggest that DA-5513 could have a beneficial effect on alcohol-induced hangovers and has the potential to ameliorate alcoholic fatty liver.

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Section: Discussionmentioning

confidence: 99%

Effects of DA-5513 on alcohol metabolism and alcoholic fatty liver in rats

Nguyen

Yong

et al. 2018

Lab Anim Res

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“…The H 2 O extracts were separately chromatographed using Diaion HP-20, YMC GEL ODS-AQ, MCI-gel CHP-20P, and Sephadex LH-20 with MeOH-H 2 O or ethanol (EtOH)-MeOH in a stepwise gradient mode. The fractions with similar high-performance liquid chromatography (HPLC) chromatograms were merged and further purified using column chromatography to obtain trapadin A (1), gallic acid (2) [19], methyl gallate (3) [19], vanillic acid (4) [19], brevifolincarboxylic acid (5) [20], ellagic acid (6) [21], urolithin A (7) [22], isourolithin A (8) [13], urolithin B (9) [11], urolithin M6 (10) [22], 1,2-di-O-galloyl-β-D-glucose (11) [23,24], 1,6-di-O-galloyl-β-D-glucose (12) [25], 1,2,3-tri-O-galloyl-β-D-glucose (13) [5,26], 1,2,6-tri-O-galloyl-β-D-glucose ( 14) [5,26], 1,2,3,6tetra-O-galloyl-β-D-glucose (15) [5,26], 1,2,3,4,6-penta-O-galloyl-β-D-glucose (16) [24,27], [28], tellimagrandin I (19) [26,29], tellimagrandin II (20) [5,26], cornusiin A (21) [30], rugosin D (22) [31], and (7 S,8 R)-dihydrodehydrodiconiferyl alcohol-9 -O-β-Dglucose (23) [5] (Figure 1). The known compounds 2-23 were identified by direct HPLC comparison with authentic standards and/or by comparing their spectral data with those reported in the literature.…”

Section: Isolation and Characterization Of Phenolic Constituentsmentioning

confidence: 99%

“…Water chestnuts from T. japonica and T. bispinosa also reportedly contain hydrolyzable tannins [ 1 , 3 , 4 , 5 ]. Biological studies have indicated diverse health benefits associated with water chestnut, including antioxidant [ 1 , 6 ], antidiabetic [ 5 , 7 , 8 ], anti-obesity [ 9 , 10 ], anti-glycation [ 5 ], anti-inflammatory [ 11 , 12 ], antiadipogenic [ 6 ], and hepatic protective effects [ 13 ]. Furthermore, it has demonstrated inhibitory activity against α-amylase [ 14 ] and α-glucosidase [ 3 , 5 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Antioxidative Hydrolyzable Tannins in Water Chestnut

Uchikura,

Miura,

Yoshimura

et al. 2023

Molecules

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Despite the various biological activities exhibited by water chestnut (the fruit of the Trapa genus), the phenolic compounds present in its extract require comprehensive characterization. Accordingly, we analyzed a 80% methanol extract of commercially available water chestnut and identified a new hydrolyzable tannin dimer termed trapadin A. Additionally, 22 known compounds, including 10 hydrolyzable tannin monomers and 2 dimers, were also detected in the extract. Spectroscopic and chemical methods were used to elucidate the structure of trapadin A, revealing it to be a hydrolyzable tannin dimer formed from units of tellimagrandin II and 1,2,3,6-tetra-O-galloyl-β-d-glucose. Moreover, the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity assay used to determine the half-maximal effective concentration values for the 23 compounds isolated from water chestnut indicated significant radical scavenging activity associated with hydrolyzable tannins. Notably, trapadin A, the new hydrolyzable tannin dimer, exhibited the highest activity value among the tested compounds.

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“…Tea bags containing water caltrop hulls are also commercially available in Taiwan. Water caltrop was reported to have hepatoprotective, antioxidative, antibacterial, anti-inflammatory, hypoglycemic, and other healthy functions [5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22]. Hydrolysable tannins are regarded as the active compounds in water caltrop hulls [5,8,11,21,22].…”

Section: Introductionmentioning

confidence: 99%

Stability of Principal Hydrolysable Tannins from Trapa taiwanensis Hulls

Wang

Chen

et al. 2019

Molecules

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The fruit and hulls of the water caltrop (Trapa taiwanensis Nakai) are used as hepatoprotective herbal tea ingredients in Taiwan. The stability of hydrolysable tannins in herbal drinks has rarely been reported. In the present study, two hydrolysable tannins, tellimagrandin II (TGII) and 1,2,3,4,6-pentagalloylglucopyranose (PGG), were isolated from water caltrop hulls. The stability of the two compounds was evaluated by treatment with various pH buffer solutions, simulated gastric fluid and intestinal fluid, different temperatures, and photo-irradiation at 352 nm in different solvents. Results showed that TGII and PGG were more stable in a pH 2.0 buffer solution (with 91.88% remaining) and in a water solution with 352 nm irradiation (with 95% remaining). TGII and PGG were more stable in methanol or ethanol solutions (with >93.69% remaining) than in an aqueous solution (with <43.52% remaining) at 100 °C. In simulated gastric fluid, more than 96% of the hydrolysable tannins remained after incubation at 37 °C for 4 h. However, these hydrolysable tannins were unstable in simulated intestinal fluid, as after incubation at 37 °C for 9 h, the content of TGII had decreased to 31.40% and of PGG to 12.46%. The synthetic antioxidants, butyl hydroxy anisole (BHA), di-butyl hydroxy toluene (BHT), and propyl gallate, did not exhibit photoprotective effects on these hydrolysable tannins. However, catechin, a natural antioxidant, displayed a weak photoprotective effect. Ascorbic acid had a short-term thermal-protective effect but not a long-term protective effect. The different stability properties of hydrolysable tannins in solutions can be used in the development of related herbal teas in the future.

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Characterization of the antioxidant fraction of Trapa japonica pericarp and its hepatic protective effects in vitro and in vivo

Cited by 7 publications

References 25 publications

Effects of DA-5513 on alcohol metabolism and alcoholic fatty liver in rats

Effects of DA-5513 on alcohol metabolism and alcoholic fatty liver in rats

Identification of Antioxidative Hydrolyzable Tannins in Water Chestnut

Stability of Principal Hydrolysable Tannins from Trapa taiwanensis Hulls

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