Characterization of the Antibacterial Activity of an SiO2 Nanoparticular Coating to Prevent Bacterial Contamination in Blood Products

Abstract

Technological innovations and quality control processes within blood supply organizations have significantly improved blood safety for both donors and recipients. Nevertheless, the risk of transfusion-transmitted infection remains non-negligible. Applying a nanoparticular, antibacterial coating at the surface of medical devices is a promising strategy to prevent the spread of infections. In this study, we characterized the antibacterial activity of an SiO2 nanoparticular coating (i.e., the “Medical Antibacterial and Antiadhesive Coating” [MAAC]) applied on relevant polymeric materials (PM) used in the biomedical field. Electron microscopy revealed a smoother surface for the MAAC-treated PM compared to the reference, suggesting antiadhesive properties. The antibacterial activity was tested against selected Gram-positive and Gram-negative bacteria in accordance with ISO 22196. Bacterial growth was significantly reduced for the MAAC-treated PVC, plasticized PVC, polyurethane and silicone (90–99.999%) in which antibacterial activity of ≥1 log reduction was reached for all bacterial strains tested. Cytotoxicity was evaluated following ISO 10993-5 guidelines and L929 cell viability was calculated at ≥90% in the presence of MAAC. This study demonstrates that the MAAC could prevent bacterial contamination as demonstrated by the ISO 22196 tests, while further work needs to be done to improve the coating processability and effectiveness of more complex matrices.

Surface modification of SiO2 nanoparticles for bacterial decontaminations of blood products

“…SiO2 NPs can be prepared by three main methods, the Stober process (using a precursor of tetraethyl orthosilicate), green synthesis, and microemulsion based on water-in-oil (W/O) reverse micelles or oil-in-water micelles [58,59]. The antimicrobial properties of SiO2NPs are most often associated with their surface functionalization, and any process that leads to their agglomeration can present a major disadvantage [60].…”

Graphene Based Membrane Modified Silica Nanoparticles for Seawater Desalination and Wastewater Treatment: Salt Rejection and Dyes

“…The maximum absorption peak corresponds to the -π* transition bond in the C=C aromatic structure and the →π* transition in the carbonyl group (C=O). The characteristic peak shifts to 270 nm for GO due to the presence of reduced graphene [19,39,40].…”

“…Graphene material has great potential as a membrane for water desalination, GO for good adsorption, and photocatalysis of water pollutants. On the other hand, SiO2 nanoparticles have good adsorption properties due to their high surface area and porosity, so they can be promoted as membrane fillers [39,40,[43][44][45][46].…”

Advanced (bio)fouling resistant surface modification of PTFE hollow-fiber membranes for water treatment

“…Next, the samples were incubated at 37°C for 48 h, following which all CFUs were determined at log 10 (CFU/mL). The reduction value (RV) and reduction percentage (R%) of the pristine and surface-modi ed PTFE HFMs was then evaluated based on the logarithmic value of antibacterial activity obtained, according to equations ( 9) and (10), respectively [49], [50].…”