Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions

Hoffman, Ari; Banapour, Babak; Levy, Jay A.

doi:10.1016/0042-6822(85)90135-7

Cited by 453 publications

(219 citation statements)

References 17 publications

Supporting

Mentioning

214

Contrasting

Order By: Relevance

“…The RT assay was performed according to Hoffman et al [34]. Briefly, the virus was purified and concentrated by ultracentrifugation of supernatant fluids (131000 x g at 4 °C for 2 h).…”

Section: Reverse Transcriptase (Rt) Assaymentioning

confidence: 99%

HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen

Legrand-Poels¹,

Hoebeke²,

Vaira³

et al. 1993

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

show abstract

“…The RT assay was performed according to Hoffman et al [34]. Briefly, the virus was purified and concentrated by ultracentrifugation of supernatant fluids (131000 x g at 4 °C for 2 h).…”

Section: Reverse Transcriptase (Rt) Assaymentioning

confidence: 99%

HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen

Legrand-Poels¹,

Hoebeke²,

Vaira³

et al. 1993

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

show abstract

“…Anti-HIV activity was measured by microtiter syncytium formation in infectious cell center assay [S], viral core protein p24 expression [6], and viral-associated reverse transcriptase (RT) activity [7].…”

Section: Anti-hiv and Ofher Crsscq~smentioning

confidence: 99%

A new class of anti‐HIV agents: GAP31, DAPs 30 and 32

et al. 1991

View full text Add to dashboard Cite

Three inhibitors of human immunodeficiency virus (HIV) have been isolated and purified to homogeneity from Euphorbiaceae himalaya seeds (Gelonium multiflorum) and carnation leaves (Dianthus caryophytlus). These proteins. GAP 31 (Gelonium Anti‐HIV Protein 31 kDa) and DAPs 30 and 32 (dianthus anti‐HIV proteins, 30 and 32 kDa), inhibit HIV‐1 infection and replication in a dosc‐dependent manner with little toxicity to target cells. The therapeutic indices of these compounds are in the order 104, suggesting that they may be clinically important agents in the treatment of AIDS. The N‐terminal amino acid sequences of these proteins show little homology to those of previously described anti‐HIV proteins. The structure‐function features of these HIV inhibitors, based on the 40–60 amino acid residues of N‐terminal sequences, are examined.

show abstract

“…Protein bands were visualized with 4-chloro-l-naphthol (Wako) as the substrate. The RT activity in RVV-infected cells was confirmed according to the method of Hoffman et al (1985).…”

Section: Cells and Virusesmentioning

confidence: 99%

Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

Hoshikawa¹,

Kojima²,

Yasuda³

et al. 1991

Journal of General Virology

View full text Add to dashboard Cite

Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions

Cited by 453 publications

References 17 publications

HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen

HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen

A new class of anti‐HIV agents: GAP31, DAPs 30 and 32

Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

Contact Info

Product

Resources

About