Characterization of the aerobic hydrocarbon-oxidizing enrichments from a high-temperature petroleum reservoir by comparative analysis of DNA- and RNA-derived clone libraries

Shestakova, N. M.; Korshunova, A. V.; Михайлова, Е. М.; Соколова, Д. Ш.; Tourova, T. P.; Belyaev, S. S.; Полтараус, А. Б.; Назина, Т. Н.

doi:10.1134/s0026261711010140

Cited by 22 publications

(24 citation statements)

References 22 publications

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“…Earlier analysis of the DNA- and RNA-derived clone libraries obtained from an enrichment culture of aerobic hydrocarbon-oxidizing bacteria of the near-bottom zone of injection well 1098, Kongdian oilfield, revealed predominance of Geobacillus subterraneus and Aeribacillus (previously Geobacillus ) pallidus in this enrichment (Shestakova et al, 2011b). …”

Section: Discussionmentioning

confidence: 89%

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

et al. 2017

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The goal of this work was to study the overall genomic diversity of microorganisms of the Dagang high-temperature oilfield (PRC) and to characterize the metabolically active fraction of these populations. At this water-flooded oilfield, the microbial community of formation water from the near-bottom zone of an injection well where the most active microbial processes of oil degradation occur was investigated using molecular, cultural, radiotracer, and physicochemical techniques. The samples of microbial DNA and RNA from back-flushed water were used to obtain the clone libraries for the 16S rRNA gene and cDNA of 16S rRNA, respectively. The DNA-derived clone libraries were found to contain bacterial and archaeal 16S rRNA genes and the alkB genes encoding alkane monooxygenases similar to those encoded by alkB-geo1 and alkB-geo6 of geobacilli. The 16S rRNA genes of methanogens (Methanomethylovorans, Methanoculleus, Methanolinea, Methanothrix, and Methanocalculus) were predominant in the DNA-derived library of Archaea cloned sequences; among the bacterial sequences, the 16S rRNA genes of members of the genus Geobacillus were the most numerous. The RNA-derived library contained only bacterial cDNA of the 16S rRNA sequences belonging to metabolically active aerobic organotrophic bacteria (Tepidimonas, Pseudomonas, Acinetobacter), as well as of denitrifying (Azoarcus, Tepidiphilus, Calditerrivibrio), fermenting (Bellilinea), iron-reducing (Geobacter), and sulfate- and sulfur-reducing bacteria (Desulfomicrobium, Desulfuromonas). The presence of the microorganisms of the main functional groups revealed by molecular techniques was confirmed by the results of cultural, radioisotope, and geochemical research. Functioning of the mesophilic and thermophilic branches was shown for the microbial food chain of the near-bottom zone of the injection well, which included the microorganisms of the carbon, sulfur, iron, and nitrogen cycles.

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Section: Discussionmentioning

confidence: 89%

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

et al. 2017

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“…2009). Crude oil degradation under thermophilic conditions (60°C) by Geobacillus strains, isolated from injection and production wells of the Daging oilfield (China), was recently reported (Shestakova et al. 2011).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous hydrocarbon biodegradation and biosurfactant production by oilfield-selected bacteria

Mnif

Chamkha

Labat

et al. 2011

Journal of Applied Microbiology

119

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Aims: To study the bacterial diversity associated with hydrocarbon biodegradation potentiality and biosurfactant production of Tunisian oilfields bacteria.;Methods and Results: Eight Tunisian hydrocarbonoclastic oilfields bacteria have been isolated and selected for further characterization studies. Phylogenetic analysis revealed that three thermophilic strains belonged to the genera Geobacillus, Bacillus and Brevibacillus, and that five mesophilic strains belonged to the genera Pseudomonas, Lysinibacillus, Achromobacter and Halomonas. The bacterial strains were cultivated on crude oil as sole carbon and energy sources, in the presence of different NaCl concentrations (1, 5 and 10%, w/v), and at 37 or 55 degrees C. The hydrocarbon biodegradation potential of each strain was quantified by GC-MS. Strain C450R, phylogenetically related to the species Pseudomonas aeruginosa, showed the maximum crude oil degradation potentiality. During the growth of strain C450R on crude oil (2%, v/v), the emulsifying activity (E24) and glycoside content increased and reached values of 77 and 1.33 g l(-1), respectively. In addition, the surface tension (ST) decreased from 68 to 35.1 mN m(-1), suggesting the production of a rhamnolipid biosurfactant. Crude biosurfactant had been partially purified and characterized. It showed interest stability against temperature and salinity increasing and important emulsifying activity against oils and hydrocarbons.;Conclusions: The results of this study showed the presence of diverse aerobic bacteria in Tunisian oilfields including mesophilic, thermophilic and halotolerant strains with interesting aliphatic hydrocarbon degradation potentiality, mainly for the most biosurfactant produced strains.;Significance and Impact of the Study: It may be suggested that the bacterial isolates are suitable candidates for practical field application for effective in situ bioremediation of hydrocarbon-contaminated sites

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“…has attracted industrial interest due to their potential applications in biotechnological processes as sources of various thermostable enzymes [50]. Shestakova and co-authors [51] also detected the presence of Geobacillus-like organisms in the alk gene RNA library and in the 16S rRNA gene library from hydrocarbonoxidizing aerobic enrichments from a high-temperature petroleum reservoir in China, showing the predominance and functional activity of geobacilli.…”

Section: Resultsmentioning

confidence: 99%

Diversity of Hydrocarbon-Related Catabolic Genes in Oil Samples from Potiguar Basin (Rn, Brazil)

Verde¹,

Silva²,

Dellagnezze³

et al. 2013

J Pet Environ Biotechnol

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Biodegradation may result in physicochemical changes in crude oil and natural gas properties, being responsible for the decrease of saturated hydrocarbons and yielding heavy oil with low economic value. Studies on the diversity of microbial catabolic genes in oil reservoirs are scarce and could help to predict the potential of a petroleum sample to be biodegraded. The aim of this study was to evaluate the diversity of genes involved in hydrocarbon degradation in Brazilian petroleum samples (biodegraded and non-biodegraded) through the construction and analysis of gene libraries (alkane monooxygenase-alk, dioxygenase-ARHDs and 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase-bamA). The results showed a differential distribution of catabolic genes between the sites, being the biodegraded oil more diverse for the alk and bamA genes. Sequences were similar to the alkB genes from Geobacillus thermoleovorans and several species of Acinetobacter, to ARHD genes from Pseudomonas spp. and two species of Burkholderia, and to bamA genes from deltaproteobacteria. Interestingly, most of the catabolic sequences recovered from both petroleum reservoirs grouped together forming distinct clusters in the phylogenetic tree reconstruction and may correspond to potentially new genes, possibly harbored by yet uncultivated microorganisms. This is the first report on the detection of alk, ARHD and bamA genes in petroleum reservoir environments, demonstrating the genetic potential of such microbial communities to biodegrade the oil.

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Characterization of the aerobic hydrocarbon-oxidizing enrichments from a high-temperature petroleum reservoir by comparative analysis of DNA- and RNA-derived clone libraries

Cited by 22 publications

References 22 publications

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

Simultaneous hydrocarbon biodegradation and biosurfactant production by oilfield-selected bacteria

Diversity of Hydrocarbon-Related Catabolic Genes in Oil Samples from Potiguar Basin (Rn, Brazil)

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