Characterization of the Activity Spectrum of MON 88702 and the Plant-Incorporated Protectant Cry51Aa2.834_16

Bachman, Pamela M.; Ahmad, Aqeel; Ahrens, Jeffrey E.; Akbar, Waseem; Baum, James A.; Brown, S.T.; Clark, Thomas L.; Fridley, Jennifer M.; Gowda, A. S. Prakasha; Greenplate, John T.; Jensen, Peter D.; Mueller, Geoffrey M.; Odegaard, Matthew L.; Tan, Jian; Uffman, Joshua P.; Levine, Steven L.

doi:10.1371/journal.pone.0169409

Cited by 44 publications

(49 citation statements)

References 49 publications

(68 reference statements)

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“…However, more recently, genetically modified cotton plants have been developed to express insecticidal activity towards L. hesperus and L. lineolaris (Baum et al, 2012;Gowda et al, 2016;Bachman et al, 2017). However, more recently, genetically modified cotton plants have been developed to express insecticidal activity towards L. hesperus and L. lineolaris (Baum et al, 2012;Gowda et al, 2016;Bachman et al, 2017).…”

Section: -------------------------------Mm --------------------------mentioning

confidence: 99%

The Cotton Boll: Temporal Susceptibility of the Boll Wall to Stylet Penetration by Hemipteran Pests

Esquivel¹,

Hinze

2019

Crop Science

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Cotton (Gossypium hirsutum L.) is a high‐value cash crop that is injured by various hemipteran insects, including lygus bugs (mirids) and stink bugs (pentatomids). Stink bugs can transmit pathogens that cause seed and boll rot of cotton, and lygus bugs can also transmit pathogens. However, the temporal susceptibility to breaching of the boll wall by stink bugs and related species was unknown. Our objective was to compare known estimates of stylet penetration for mirids (Lygus lineolaris Palisot de Beauvois and Pseudatomoscelis seriatus Reuter) and pentatomids [Chinavia hilaris Say, Euschistus obscurus (Palisot), Euschistus servus (Say), Euschistus tristigmus (Say), Nezara viridula (L.), Oebalus pugnax (F.), Piezodorus guildinii Westwood, and Thyanta custator accerra McAtee] against observed minimum boll wall thickness in multiple entries from four Gossypium species to determine the time at which bolls initially become susceptible to stylet penetration. Results show that P. seriatus could not breach the boll wall. Lygus lineolaris could breach the wall at ≤7 d after flowering in G. herbaceum L. and in select entries of G. hirsutum. All the pentatomids could breach the boll wall at 1 d after flowering, and virtually all could continue to breach the wall throughout the entire production season. Monitoring efforts for stink bugs may need to be implemented at boll set to mitigate introduction of pathogens and yield losses given the season‐long susceptibility to stink bugs.

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Section: -------------------------------Mm --------------------------mentioning

confidence: 99%

The Cotton Boll: Temporal Susceptibility of the Boll Wall to Stylet Penetration by Hemipteran Pests

Esquivel¹,

Hinze

2019

Crop Science

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“…An example in this system is a new transgenic cotton cultivar which has been developed to be resistant to Lygus spp. plant bugs but may also be resilient to other mirids, including P. seriatus . If there are genotypes of P. seriatus that are associated with cotton and that do not have gene flow with genotypes associated with other host plants, then the cotton‐associated genotypes could potentially evolve resistance to the transgenic cotton, as would be expected with chronic exposure to any new transgenic or insecticidal control .…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide markers reveal temporal instability of local population genetic structure in the cotton fleahopper, Pseudatomoscelis seriatus (Hemiptera: Miridae)

Raszick

Suh

Dickens³

et al. 2019

Pest Management Science

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BACKGROUND The cotton fleahopper, Pseudatomoscelis seriatus (Reuter) (Hemiptera: Miridae), is a pest of upland cotton, Gossypium hirsutum L. (Malvales: Malvaceae), that attacks pre‐floral buds (squares), leading to abscission and yield losses. In the Brazos Valley cotton production area of Texas (USA), P. seriatus exhibits a seasonal pattern of host use. In spring, eggs hatch from stems of the overwintering host, woolly croton, Croton capitatus Michx. (Malpighiales: Euphorbiaceae). During the growing season, individuals feed on a variety of host plants, including cotton. Adults return to woolly croton at season end to oviposit. We investigated if genetic differentiation exists between populations infesting cotton and those infesting alternative hosts, and whether woolly croton serves as a year‐end site of admixture that could be suitable as a natural refuge for the purposes of insect resistance management. We combined high‐throughput DNA sequencing with fine‐scale spatio‐temporal sampling to test (i) whether a population genomic approach would recover patterns of genetic variation consistent with earlier studies and (ii) if local genetic population structure was robust to seasonal changes in local habitat over time. RESULTS We found high gene flow among populations of P. seriatus collected from different host plants in the Brazos Valley. We also identified temporal instability of the local population genetic structure, including the near complete loss of a genotypic group that had been previously abundant. CONCLUSION We support the status of woolly croton as a natural refuge that promotes year‐end gene flow between genotypes infesting cotton and those infesting alternative hosts. © 2019 Society of Chemical Industry

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“…This result suggests that adult females of both species can detect and avoid the presence of MON 88702 expressed in cotton seedlings. Because MON 88702 had limited acute adult toxicity, these behavioral differences may be the most significant factor driving differences in F. fusca damage observed in field studies measuring efficacy …”

Section: Resultsmentioning

confidence: 99%

“…Bayer has developed MON 88702 cotton, the first crop that expresses Cry51Aa2.834_16 Bt toxin to control targeted hemipteran and thysanopteran crop pests . The Cry51Aa2.834_16 Bt toxin (hereafter referred to as MON 88702) expressed in cotton was modified to selectively enhance insecticidal activity against piercing‐sucking pests .…”

Section: Introductionmentioning

confidence: 99%

“…Field trials have demonstrated that cotton seedlings expressing MON 88702 support lower F. fusca adult and larval infestations and sustain less feeding damage through the period of susceptibility from seedling emergence through the 4–5 true‐leaf stage . Further study of population development in a choice experiment revealed that the presence of MON 88702 was associated with reduced oviposition when compared to non‐ Bt cotton .…”

Section: Introductionmentioning

confidence: 99%

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Novel mechanism of thrips suppression by Cry51Aa2.834_16 Bt toxin expressed in cotton

Huseth

Yorke

Head

et al. 2019

Pest Management Science

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BACKGROUND Genetically engineered (GE) crops that express insecticidal traits have improved the sustainability of insect pest management worldwide, but many important pest orders are not controlled by commercially available toxins. Development of the first transgenic thysanopteran‐ and hemipteran‐active Bacillus thuringiensis (Bt) Cry51Aa2.834_16 toxin expressed in MON 88702 cotton will significantly expand the diversity of pests controlled in the crop. Here, we examined MON 88702 cotton activity against two thrips species within the same genera, Frankliniella fusca and Frankliniella occidentalis. We used a multi‐component cotton tissue assay approach to understand effects on adult longevity, fecundity, and larval development. RESULTS We found that in no‐choice assays, cotton plants expressing MON 88702 suppress oviposition, when compared to a non‐Bt cotton. MON 88702 did not kill a large proportion of F. fusca larvae or adults but killed most F. occidentalis larvae. Time series experiments with F. occidentalis larvae documented significant developmental lags for MON 88702 exposed individuals. We also found that female thrips preferred to oviposit on non‐Bt cotton when provided a choice. CONCLUSION Together these results describe the activity of MON 88702 against thrips. They document clear differences in toxin performance between different thrips species and throughout the insects' life cycle. Most importantly, we show that MON 88702 was associated with reduced oviposition via behavioral avoidance to the toxin. This is a novel mechanism of action for pest control for a Bt crop plant. Together, these results provide a basis to describe the mechanism of population control for MON 88702 cotton. © 2019 Society of Chemical Industry

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Characterization of the Activity Spectrum of MON 88702 and the Plant-Incorporated Protectant Cry51Aa2.834_16

Cited by 44 publications

References 49 publications

The Cotton Boll: Temporal Susceptibility of the Boll Wall to Stylet Penetration by Hemipteran Pests

The Cotton Boll: Temporal Susceptibility of the Boll Wall to Stylet Penetration by Hemipteran Pests

Genome‐wide markers reveal temporal instability of local population genetic structure in the cotton fleahopper, Pseudatomoscelis seriatus (Hemiptera: Miridae)

Novel mechanism of thrips suppression by Cry51Aa2.834_16 Bt toxin expressed in cotton

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