Characterization of the ABA Receptor VlPYL1 That Regulates Anthocyanin Accumulation in Grape Berry Skin

Gao, Zhen; Li, Qin; Li, Jing; Chen, Yujin; Luo, Meng; Li, Hui; Wang, Jiyuan; Wu, Yusen; Duan, Shuyan; Wang, Lei; Song, Shiren; Xu, Wenping; Zhang, Caixi; Wang, Shiping; Ma, Chao

doi:10.3389/fpls.2018.00592

Cited by 35 publications

(25 citation statements)

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“…Changes of polyphenol contents and gene expressions of vital enzymes involved in polyphenol biosynthesis under ABA and MLT treatments were presented in Figure 5. ABA has been widely recognized as a promotor to polyphenolic biosynthesis, and the increase in the expression of ABA receptor VIPYL1 led to the accumulation of anthocyanin and a series of ABA-responsive gene transcripts in grape berries [30]. The expression of key enzymes in the anthocyanin biosynthesis pathway, including PAL, C4H, CHS, CHI, F3H, F3 H, DFR, and LDOX, was up-regulated by exogenous ABA in litchi [16] and strawberry [20].…”

Section: Discussionmentioning

confidence: 99%

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

et al. 2019

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Grape polyphenols contributing to more than half of the global polyphenol market were well studied; however, how melatonin (MLT), a potential plant hormone, and abscisic acid (ABA) affects polyphenols profile is still poorly understood. To explore whether these hormones are involved in polyphenolic biosynthesis, grape (Vitis vinifera cv. Kyoho) was exposed to MLT, ABA, and NDGA (nordihydroguaiaretic acid, an ABA biosynthesis inhibitor) treatments, and 16 polyphenols were identified from grape extracts by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS). Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component, especially catechin, which was almost increased double by 200 µM of MLT treatment. Furthermore, the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well but were down-regulated by NDGA. The present study provided new insights for improving flavonoids accumulation in agricultural production and its underlying mechanism.

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Section: Discussionmentioning

confidence: 99%

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

et al. 2019

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“…This challenge may explain, at least in part, the lack of functional studies using these grape infectious viral vectors. Other viral vectors like Tobacco rattle virus (TRV) and Apple latent spherical virus (ASLV) vectors can provoke VIGS in many plant species (Senthil-Kumar and Mysore, 2011) but not in grapevine, as recently confirmed by Gao et al (2018) who failed to silence VlPYL1 (pyrabactin resistance/PYL (PYR-like)) using the TRV-mediated silencing in "Kyoho" grapes. To the best of our knowledge, only one viral-derived vector was successfully used to perform functional studies in grapevine.…”

Section: Virus-derived Technologiesmentioning

confidence: 95%

“…This method is derived from the transient methodology successfully applied to tomato fruit (Orzaez et al, 2006) based on Agrobacterium cultures injection through the fruit stylar apex using a syringe with needle. Using this transient overexpression technique in the "Kyoho" grape fruit, Gao et al (2018) showed the involvement of the ABA Receptor VlPYL1 in the regulation of anthocyanin accumulation in grape berry skin. Similarly, Sun et al (2017) showed that VvVHP1;2 (vacuolar H + -PPase 1) overexpression promoted anthocyanin accumulation Lorenza Dalla Costa et al in berry skins.…”

Section: Agrobacterium-derived Technologiesmentioning

confidence: 99%

The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

et al. 2019

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Context of the review:The manipulation of the genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies. In the last 15 years, considerable knowledge has accumulated about the grapevine genome as well as the mechanisms involved in the interaction of the vine with the environment, pests and diseases. Despite the difficulties associated with genetic mapping in this species (allele diversity, chimerism, long generation intervals…), several major QTLs (quantitative trait loci) controlling important vegetative or reproductive traits have been identified. Considering the huge genotypic and phenotypic diversities existing in Vitis, breeding offers a substantial range of options to improve the performances of cultivars. However, even if marker-assisted selection was largely developed to shorten breeding programs, the selection of improved cultivars, whether for agronomic traits or disease tolerances, is still long and uncertain. Moreover, breeding by crossing does not preserve cultivar genetic background, when the wine industry and market are still based on varietal wines. Significance of the review: In grapevine, pioneering biotechnologies were set up in the 1960s to propagate and/or clean the material from micro-organisms. In the 1990s, the basis of genetic engineering was primary established through biolistic or Agrobacterium with several derived technologies refined in the last 10 years. The latest advance is represented by a group of technologies based on genome editing which allows a much more precise modification of the genome. These technologies, so-called NBTs (new breeding technologies), which theoretically do not deconstruct the phenotype of existing cultivars, could be potentially better accepted by the wine industry and consumers than previous GMO (genetically modified organism) approaches. This paper reviews the current state-ofthe-art of the biotechnologies available for grapevine genome manipulation and future prospects for genetic improvement.

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“…It was reported that SRPBCC domains have a deep hydrophobic ligand-binding pocket that can bind diverse ligands and spans all three kingdoms of life, but its function remains unclear. Moreover, SRPBCC-containing genes play a wide range of roles, including strawberry fruit ripening [22] , regulation of anthocyanin accumulation [23] , encoding type II secretion chaperones [24] , functioning in the stabilization of mRNA [25] , catalyzing the free radical reactions of polyunsaturated fatty acids [26] , and α-pyridone ring formation [27] . Hydroxylases that hydroxylate the C6 of pyridine, such as 3-succinoylpyridine dehydrogenase (SpmABC) [28] and picolinic acid dehydrogenase (PicA) [29] , include SRPBCC-containing components and are fused with [2Fe-2S] clusters, whereas most pyridine hydroxylases, such as NdhLSM and KdhLMS, have no SRPBCC domain (Figure 4A).…”

Section: Resultsmentioning

confidence: 99%

Catabolism of 3-hydroxypyridine byEnsifer adhaerensHP1: a novel four-component gene encoding 3-hydroxypyridine dehydrogenase HpdA catalyzes the first step of biodegradation

Wang

Ren

et al. 2020

Preprint

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Abstract3-Hydroxypyridine (3HP) is an important natural pyridine derivative. Ensifer adhaerens HP1 can utilize 3HP as the sole source of carbon, nitrogen and energy to grow. However, the genes responsible for the degradation of 3HP remain unknown. In this study, we predicted that a gene cluster, designated 3hpd, may be responsible for the degradation of 3HP. The initial hydroxylation of 3HP is catalyzed by a four-component dehydrogenase (HpdA1A2A3A4), leading to the formation of 2,5-dihydroxypyridine (2,5-DHP) in E. adhaerens HP1. In addition, the SRPBCC component in HpdA existed as a separate subunit, which is different from other SRPBCC-containing molybdohydroxylases acting on N-heterocyclic aromatic compounds. Our findings provide a better understanding of the microbial degradation of pyridine derivatives in nature. Additionally, research on the origin of the discovered four-component dehydrogenase with a separate SRPBCC domain may be of great significance.Importance3-Hydroxypyridine is an important building block for synthesizing drugs, herbicides and antibiotics. Although the microbial degradation of 3-hydroxypyridine has been studied for many years, the molecular mechanisms remain unclear. Here, we show that 3hpd is responsible for the catabolism of 3-hydroxypyridine. The 3hpd gene cluster was found to be widespread in Actinobacteria, Rubrobacteria, Thermoleophilia, and Alpha-, Beta-, and Gammaproteobacteria, and the genetic organization of the 3hpd gene clusters in these bacteria showed high diversity. Our findings provide new insight into the catabolism of 3-hydroxypyridine in bacteria.

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Characterization of the ABA Receptor VlPYL1 That Regulates Anthocyanin Accumulation in Grape Berry Skin

Cited by 35 publications

References 87 publications

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

Catabolism of 3-hydroxypyridine byEnsifer adhaerensHP1: a novel four-component gene encoding 3-hydroxypyridine dehydrogenase HpdA catalyzes the first step of biodegradation

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