Characterization of tetracycline and methicillin resistant Staphylococcus aureus strains in a Spanish hospital: Is livestock-contact a risk factor in infections caused by MRSA CC398?

Molecular characterization of Staphylococcus aureus isolated from humans related to a livestock farm in Spain, with detection of MRSA-CC130 carrying mecC gene: A zoonotic case?

“…In addition, the genes encoding leukocidines DE (luk-DE) and M (lukM), the biofilm-associated protein (bap), and the collagenbinding protein (cna) were also studied by PCR. 6 The five genes that comprise the IEC cluster (scn, chp, sak, and sea or sep) were studied by PCR. Depending on the presence or absence of these genes and their different combinations, S. aureus isolates were classified into 7 different IEC types according to patterns previously described.…”

“…6,7 They have been increasingly detected in animals or humans, either as colonizer or causing infections. 6,8 In 2011, a new mecA homologue was described in the S. aureus isolate LGA251 from an epidemiological study of bovine mastitis in England.…”

“…6,7 They have been increasingly detected in animals or humans, either as colonizer or causing infections. 6,8 In 2011, a new mecA homologue was described in the S. aureus isolate LGA251 from an epidemiological study of bovine mastitis in England. 10 This new resistance determinant shared homology of 70% over the mecA and was named as mecA LGA251 , 9 and renamed in 2012 as mecC 10 and was integrated into the new chromosomal cassette SCCmec-XI.…”

Livestock-Associated, Antibiotic-Resistant Staphylococcus aureus Nasal Carriage and Recent Skin and Soft Tissue Infection among Industrial Hog Operation Workers

“…aureus is not typically conducted for clinical SSTI cases [21,22]. To date, prospective cohort studies of healthy volunteer hog production workers have not identified risk factors for SSTI other than livestock exposure [21,23,24]. Identifying an association between SSTI and nasal carriage, which can be managed through decolonization interventions [25], could help efforts to prevent S .…”

Clonal lineages detected amongst tetracycline-resistant meticillin-resistant Staphylococcus aureus isolates of a Tunisian hospital, with detection of lineage ST398

“…However, no MRSA ST398 isolates have been identified in hospitals in Tunisia. Previous studies carried out in Spain (Benito et al, 2014;Camoez et al, 2013;Lozano et al, 2012) used tetracycline resistance as a phenotypic marker for the detection of LA-MRSA isolates, amongst them ST398, in hospital environments. The aims of this study were to perform molecular typing in a cohort of MRSA isolates obtained from patients hospitalized in the Military Hospital of Tunis (Tunisia), selected according to their phenotype of tetracycline resistance, as well as to determine if tetracycline resistance is a good marker for detection of CC398 in Tunisia.…”