Characterization of Tannin-tolerant Bacterial Isolates from East African Ruminants

Odenyo, A.A.; Bishop, Richard P.; Asefa, Genet; Jamnadass, Ramni; Odongo, David; Osuji, P.O.

doi:10.1006/anae.2000.0367

Cited by 42 publications

(39 citation statements)

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“…The data also suggest that the technique may selectively result in isolation of coding sequences. The 16S ribosomal DNA sequences of the S. ruminantium isolates used in this study were 99% identical, and they clustered closely in dendrograms derived from genome-wide amplified fragment length polymorphism analysis (10).…”

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confidence: 67%

“…We have previously reported the characterization of Selenomonas ruminantium isolates, capable of growing in 70 g of tannic acid per liter, from African ruminants, bush duiker (Sylvicapra grimmia), goat (Capra hircus), and sheep (Aries ova). Five of these isolates were identified as S. ruminantium on the basis of fermentation of carbohydrates and of comparison of the 16S ribosomal DNA sequences to those of reference isolates (9,10). Due to our interest in transferring tannin-tolerant bacteria from wildlife into domestic ruminants to improve their ability to digest tannin-rich feeds, we isolated DNA sequences unique to individual S. ruminantium isolates for development of probes to monitor persistence in the rumen following inoculation into novel hosts.…”

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confidence: 99%

“…S. ruminantium EAT2 from bush duiker was selected as the "tester" because it exhibited the highest levels of tannin tolerance and unlike the other isolates could hydrolyze gallic acid (9). The "driver" DNA was from an Ethiopian goat isolate (EG19) genetically closely related to EAT2 (10). Nine sequences were obtained by applying SSH to genomic DNA according to the manufacturer's instructions, except that the first and second subtractive hybridizations were performed at 62°C instead of 63°C, and were cloned into a pGem T vector (Promega).…”

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confidence: 99%

“…1B. The primers were also applied to total DNA that had been prepared from the gastrointestinal contents of wild and domestic ungulates by a physical disruption method with zirconia silicon beads and a bead beater (11), collected as described previously (8,9,10). Neither set of primers generated any products from 300 ng of total DNA prepared from 10 l of rumen fluid from five different Bos indicus cattle.…”

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confidence: 99%

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Specific PCR Assay for a Tannin-Tolerant Selenomonas ruminantium Isolate, Derived from Helicase Coding Sequences

Bishop

Obura

Odongo

et al. 2004

Appl Environ Microbiol

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Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.Tannins diminish protein and dry matter digestibility in ruminants (4). The tolerance of certain mammalian species for hydrolyzable tannins may be attributable to tannin-resistant bacteria in the rumen. Tannin-tolerant bacteria have been isolated from Enterobacteriaceae and the genera Selenomonas and Streptococcus (7,9,12). We have previously reported the characterization of Selenomonas ruminantium isolates, capable of growing in 70 g of tannic acid per liter, from African ruminants, bush duiker (Sylvicapra grimmia), goat (Capra hircus), and sheep (Aries ova). Five of these isolates were identified as S. ruminantium on the basis of fermentation of carbohydrates and of comparison of the 16S ribosomal DNA sequences to those of reference isolates (9, 10). Due to our interest in transferring tannin-tolerant bacteria from wildlife into domestic ruminants to improve their ability to digest tannin-rich feeds, we isolated DNA sequences unique to individual S. ruminantium isolates for development of probes to monitor persistence in the rumen following inoculation into novel hosts. We used genome-wide suppression subtractive hybridization (SSH) PCR (2, 3) as implemented in the PCR-Select Bacterial Genome Subtraction Kit (Clontech). S. ruminantium EAT2 from bush duiker was selected as the "tester" because it exhibited the highest levels of tannin tolerance and unlike the other isolates could hydrolyze gallic acid (9). The "driver" DNA was from an Ethiopian goat isolate (EG19) genetically closely related to EAT2 (10). Nine sequences were obtained by applying SSH to genomic DNA according to the manufacturer's instructions, except that the first and second subtractive hybridizations were performed at 62°C instead of 63°C, and were cloned into a pGem T vector (Promega). The sequences were evaluated for specificity by hybridization to slot blots of DNA (100 ng) from five tannin-tolerant and two S. ruminantium reference isolates. At a washing stringency of 0.5ϫ SSC (1ϫ SSC is 0.15 M NaCl plus 0.015 M sodium citrate)-0.1% sodium dodecyl sulfate, all nine cloned sequences hybridized most strongly to EAT2 and variably to the other six isolates.Two of the nine sequences hybridized specifically to EAT2 DNA. Results for clone number 1 are shown in Fig. 1A.The nucleotide sequences of the two EAT2-specific sequences and of a third clone that hybridized more strongly to EAT2 than to other isolates were determined. Blast X searches of the NCBI databases revealed that clones 1 (524 bp) and 3 (941 bp) contained open reading frames (ORFs) with significant amino acid identity to different mobile element or prophage-encoded ORFs. For clone 1 there was very high ide...

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confidence: 67%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Specific PCR Assay for a Tannin-Tolerant Selenomonas ruminantium Isolate, Derived from Helicase Coding Sequences

Bishop

Obura

Odongo

et al. 2004

Appl Environ Microbiol

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“…Unfortunately, the secretion of tannin-binding salivary proteins has not been investigated in sika deer. However, tannin-tolerant bacteria that degrade hydrolyzable tannin have been isolated from rumen microflora of sika deer (Sawabe 2006) as well as bush duiker Sylvicapra grimmia, giraffe Giraffa camelopardalis, Grant's gazelle Gazella granti, sheep, and goats (Odenyo et al 2001). Although the efficiency of tannin--tolerant bacteria has not been quantified, it is possible that the effect of phenolics on the regression lines of DN to FN may be mitigated in the diet of sika deer.…”

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confidence: 99%

Fecal nitrogen as an index of dietary nitrogen in two sika deerCervus nippon populations

et al. 2007

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Gut microbiota of skywalker hoolock gibbons (Hoolock tianxing) from different habitats and in captivity: Implications for gibbon health

Lan

Hong

Gao

et al. 2023

American J Primatol

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The gut microbiota plays an integral role in the metabolism and immunity of animal hosts, and provides insights into the health and habitat assessment of threatened animals. The skywalker hoolock gibbon (Hoolock tianxing) is a newly described gibbon species, and is considered an endangered species. Here, we used 16S rRNA amplicon sequencing to describe the fecal bacterial community of skywalker hoolock gibbons from different habitats and in captivity. Fecal samples (n = 5) from two captive gibbons were compared with wild populations (N = 6 gibbons, n = 33 samples). At the phylum level, Spirochetes, Proteobacteria, Firmicutes, Bacteroidetes dominated in captive gibbons, while Firmicutes, Bacteroidetes, and Tenericutes dominated in wild gibbons. At the genus level, captive gibbons were dominated by Treponema-2, followed by Succinivibrio and Cerasicoccus, while wild gibbons were dominated by Anaeroplasma, Prevotellaceae UCG-001, and Erysipelotrichaceae UCG-004. Captive rearing was significantly associated with lower taxonomic alphadiversity, and different relative abundance of some dominant bacteria compared to wild gibbons. Predicted Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that captive gibbons have significantly lower total pathway diversity and higher relative abundance of bacterial functions involved in "drug resistance: antimicrobial" and "carbohydrate metabolism" than wild gibbons. This study reveals the potential influence of captivity and habitat on the gut bacterial community of gibbons and provides a basis for guiding the conservation management of captive populations.

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Characterization of Tannin-tolerant Bacterial Isolates from East African Ruminants

Cited by 42 publications

References 22 publications

Specific PCR Assay for a Tannin-Tolerant Selenomonas ruminantium Isolate, Derived from Helicase Coding Sequences

Specific PCR Assay for a Tannin-Tolerant Selenomonas ruminantium Isolate, Derived from Helicase Coding Sequences

Fecal nitrogen as an index of dietary nitrogen in two sika deerCervus nippon populations

Gut microbiota of skywalker hoolock gibbons (Hoolock tianxing) from different habitats and in captivity: Implications for gibbon health

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