Characterization of T-cell Non-Hodgkin's Lymphoma and its Association with Epstein-Barr Virus in Pakistani Patients

Noorali, Samina; Pervez, Shahid; Moatter, Tariq; Soomro, Irshad; Kazmi, Shahana Urooj; Nasir, Muhammad; Smith, Joan K.

doi:10.1080/1042819031000067747

Cited by 9 publications

(9 citation statements)

References 32 publications

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“…Under these blinded conditions, we detected IGH clones in only 6 of 74 (8%) PTCL cases, consistent with previous reports on large series of PTCL (9 to 16%). [2][3][4][5][6] By contrast, we detected IGH clones in 23 of 24 (96%) pre-B-ALL cases, and similarly high rates of detection have been reported for pre-B-ALL. 18 -20 In regard to PTCL, the frequency of IGH clonality differed significantly from our previous report, in which we detected IGH clones in 33% of AILT cases and 35% of PTCL-U cases (P Ͻ 0.004).…”

Section: Discussionmentioning

confidence: 61%

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The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large Cell Lymphoma and Other Peripheral T-Cell Lymphomas

Tan

Seo

Warnke

et al. 2008

The Journal of Molecular Diagnostics

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We previously identified a relatively high frequency of B-cell proliferations along with simultaneous T-cell receptor ␥-chain gene (TRG) and immunoglobulin heavy chain gene (IGH) rearrangements in a seriesPeripheral T-cell lymphoma (PTCL) is an uncommon malignancy that accounts for less than 10% of non-Hodgkin lymphomas worldwide. By current World Health Organization criteria, the diagnosis and classification of PTCL is based on the combination of clinical, histologic, immunophenotypic, and genetic findings.1 However, the diagnosis of PTCL is difficult even for experienced pathologists, given the infrequency of cases, the often unusual histologic features, and perhaps most importantly, the lack of good immunophenotypic markers to assess for clonality in T-lineage neoplasms.In cases of suspected PTCL, pathologists often evaluate for clonality and assess for lineage by performing PCR-based assays for clonal rearrangement in both the T-cell receptor ␥-chain gene (TRG) and the immunoglobulin heavy chain gene (IGH). Although PCR-based assays for TRG typically detect clones in 80 to 90% of PTCL cases, several studies have shown that IGH clones are also detected relatively frequently (9 to 16%).2-6 The detection of an IGH clone, with or without a concurrent TRG clone, complicates an already challenging diagnosis.Recently, we investigated the etiology of IGH clones in PTCL by studying a large series of two of the most common subtypes of PTCL, angioimmunoblastic T-cell lymphoma (AILT) and PTCL-unspecified (PTCL-U).7 A subset of cases were complicated by associated B-cell proliferations, a finding that we and others have described as an atypical infiltrate of B cells that is often associated with Epstein-Barr virus (EBV). 8 -11 Using multiplex PCRs developed in a European collaborative study (BIOMED-2), we detected TRG clones in approximately 80% of cases and IGH clones in approximately 35% of cases, with the majority of IGH clones detected along with simultaneous TRG clones.Interestingly, a positive IGH clone correlated, at least in part, with the presence of a B-cell proliferation, suggesting that B-cell proliferations contribute to IGH clonality. However, alternate explanations for the detection of simultaneous TRG and IGH clones include those that are technical in nature and so-called lineage infidelity. In this context, lineage infidelity refers to recombination of both TRG and IGH in the same clone. However, this phenomenon is more common in immature hematolymphoid neoplasms and occurs only rarely (Ͻ5%) in mature B-and T-cell non-Hodgkin lymphomas. [12][13][14][15] Supported by the Stanford Department of Pathology.

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Section: Discussionmentioning

confidence: 61%

“…Although PCR-based assays for TRG typically detect clones in 80 to 90% of PTCL cases, several studies have shown that IGH clones are also detected relatively frequently (9 to 16%). [2][3][4][5][6] The detection of an IGH clone, with or without a concurrent TRG clone, complicates an already challenging diagnosis.…”

mentioning

confidence: 99%

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large Cell Lymphoma and Other Peripheral T-Cell Lymphomas

Tan

Seo

Warnke

et al. 2008

The Journal of Molecular Diagnostics

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“…In particular, studies evaluating large numbers of PTCLs by PCR show that simultaneous B-and T-cell clones occur relatively often (9 to 16%). [2][3][4][5][6] These cases, with both B-and T-cell clones, present a diagnostic dilemma for the pathologist and treating oncologist.…”

mentioning

confidence: 99%

The Frequency of B- and T-Cell Gene Rearrangements and Epstein-Barr Virus in T-Cell Lymphomas

Tan

Warnke

Arber

2006

The Journal of Molecular Diagnostics

115

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We report on a series of 58 cases of angioimmunoblastic T-cell lymphoma (AILT) and 59 cases of peripheral Tcell lymphoma, unspecified (PTCL-NOS). Subsets of cases from both diagnostic groups were complicated by associated B-cell proliferations, and we performed Band T-cell clonality studies and in situ hybridization for Epstein-Barr virus (EBV) to investigate the relationship between B-cell proliferation, B-cell clonality, and EBV. Using multiplex polymerase chain reaction assays based on the BIOMED-2 collaborative study, we detected TCR␥ T-cell clones in 78 and 81% of AILT and PTCL-NOS cases, respectively, and IGH B-cell clones in 34 and 35% of AILT and PTCL-NOS cases, respectively. The majority of cases contained EBV-positive cells, including 50% of AILT and 57% of PTCL-NOS cases, and cases with B-cell proliferations were more often EBV-positive. Although a relatively high rate of B-cell clonality has been shown for AILT, our findings for PTCL-NOS differ from previous reports in that B-cell clonality was relatively frequent. Overall, a positive B-cell clone correlated, in part, with the presence of a B-cell proliferation but not with EBV. Our findings demonstrate that B-cell clonality is a common finding in AILT and PTCL-NOS, and its presence should not negate the diagnosis established by morphologic, immunophenotypic, and clinical findings.

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“…In the Southeast Asian population, in which EBV-positive T-cell lymphoma is more common, Huh and colleagues [10], Jung and colleagues [11], Ko and colleagues [14], and Kim and colleagues [35] have reported 58%, 46.7%, 76%, and 50% of EBV-positive cases in the Korean adult population, respectively. Meanwhile, Noorali and colleagues [16] have observed a 55.4% EBV association in adult patients from Pakistan; Hirose and colleagues [21] a 63% EBV association in a Japanese adult series; and Agarwal and colleagues [36] a 8.7% EBV association in a T-cell anaplastic large cell lymphoma series from India. Conversely, in United States, Herling and colleagues [37] described the absence of EBV in 48 anaplastic T-cell lymphoma patients.…”

Section: Discussionmentioning

confidence: 99%

Low Frequency of Epstein Barr Virus Association and High Frequency of p53 Overexpression in an Argentinean Pediatric T-Cell Lymphoma Series

et al. 2009

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T-cell non-Hodgkin's lymphomas (NHLs) represent 10% to 15% of all diagnosed lymphomas in Western countries. Various geographic frequencies of T-cell NHL have been documented, in part reflecting increased exposure to pathogenic factors such as Epstein-Barr virus (EBV). Our aims were to assess EBV and p53 expression in Argentine pediatric T-cell lymphoma and to correlate them with patients' survival. Epstein-Barr encoded RNAs (EBERs) in situ hybridization and LMP1 and p53 immunohistochemical staining were performed on formalin-fixed paraffin-embedded lymph node biopsies from 25 pediatric T-lymphoma patients. In 17 of 25 samples good-quality DNA was obtained, and EBER polymerase chain reaction was assessed to confirm in situ hybridization and immunohistochemical results. Epstein-Barr virus expression was found in 8.0% of cases. p53-positive staining was distributed in 92% of pediatric cases. Kaplan-Meier survival analysis showed that neither EBV nor p53 expression was statistically significantly associated with event-free survival. Our data showed a low frequency of EBV association with pediatric T-cell lymphoma. It seems that p53 plays an important role in proliferation in our studied population, since it is overexpressed in 92% of T-cell lymphoma cases.

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Characterization of T-cell Non-Hodgkin's Lymphoma and its Association with Epstein-Barr Virus in Pakistani Patients

Cited by 9 publications

References 32 publications

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large Cell Lymphoma and Other Peripheral T-Cell Lymphomas

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large Cell Lymphoma and Other Peripheral T-Cell Lymphomas

The Frequency of B- and T-Cell Gene Rearrangements and Epstein-Barr Virus in T-Cell Lymphomas

Low Frequency of Epstein Barr Virus Association and High Frequency of p53 Overexpression in an Argentinean Pediatric T-Cell Lymphoma Series

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