Characterization of syringe-pump-driven versus pressure-driven microfluidic flows

Zeng, Wen; Li, Songjing; Wang, Zuwen

doi:10.1109/fpm.2015.7337207

Cited by 28 publications

(23 citation statements)

References 16 publications

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“…Instead of typical syringe pumps, a pressure-controlled pump (OB1, Elveflow, Paris, France) was used in this study to deliver both the continuous phase (i.e., oil) and the dispersed phase (i.e., water) at a constant flow. Compared to syringe pumps, pressure-controlled pumps can provide more stable and pulseless flow, handle much larger volume (up to several liters), and make it easier for the systems to be integrated with other microfluidic components as well as to build compact platforms [28]. Deionized water with red color dye and mineral oil (Zhanhong Chemical Industry, Guangzhou, China) mixed with a surfactant (Span-80, 7.5 g/L, Sigma-Aldrich, St. Louis, MI, USA) were used as the dispersed and continuous phase, respectively.…”

Section: Droplet Generationmentioning

confidence: 99%

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

et al. 2019

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There have been growing interests in droplet-based microfluidics due to its capability to outperform conventional biological assays by providing various advantages, such as precise handling of liquid/cell samples, fast reaction time, and extremely high-throughput analysis/screening. The droplet-based microfluidics utilizes the interaction between the interfacial tension and the fluidic shear force to break continuous fluids into uniform-sized segments within a microchannel. In this paper, the effect of different viscosities of carrier oil on water-in-oil emulsion, particularly how droplet size and droplet generation rate are affected, has been investigated using a commonly used T-junction microfluidic droplet generator design connected to a pressure-controlled pump. We have tested mineral oils with four different viscosities (5, 7, 10, and 15 cSt) to compare the droplet generation under five different flow pressure conditions (i.e., water flow pressure of 30–150 mbar and oil flow pressure of 40–200 mbar). The results showed that regardless of the flow pressure levels, the droplet size decreased as the oil viscosity increased. Average size of the droplets decreased by approximately 32% when the viscosity of the oil changed from 5 to 15 cSt at the flow pressure of 30 mbar for water and 40 mbar for oil. Interestingly, a similar trend was observed in the droplet generation rate. Droplet generation rate and the oil viscosity showed high linear correlation (R2 = 0.9979) at the water flow pressure 30 mbar and oil flow pressure 40 mbar.

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Section: Droplet Generationmentioning

confidence: 99%

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

et al. 2019

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“… 21 However, their method relied on a multi-casting PDMS process and on the use of syringe pump-driven flow, with longer stabilization time compared to pressure-driven flow. 22 This precludes stable and reproducible processing of small sample volumes. Furthermore, a significant decline in cell viability appeared for high flow rates and heterogeneous delivery, cargo- and cell-size dependent, was observed.…”

Section: Introductionmentioning

confidence: 99%

Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation

et al. 2021

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“…8b, c. The formation of hexadecane (O) droplets in water (W) is realized by maintaining the hexadecane flow rate (Q HD ) constant and varying the water flow rate (Q W ) in which 2% Tween80 (v/v) is added so as to avoid merging of HD droplets. The main reason of maintaining Q HD constant is that this fluid is controlled by a syringe pump and thus more equilibration time is required to stabilize the system (Zeng et al 2015). In consequence, the stabilization times of 30 and 5 min have been used for Q HD and Q W , respectively.…”

Section: Validation Of the Lfcs In A Droplet Generation Systemmentioning

confidence: 99%

Highly integrated polymeric microliquid flow controller for droplet microfluidics

Etxebarria

Berganzo

Brivio

et al. 2017

Microfluid Nanofluid

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and replaceable from the external electronic and pneumatic actuators box. Functionality of the LFCS is tested by connecting it to a microfluidic droplet generator, rendering highly stable flow rates and allowing generation of monodisperse droplets over a wide range of flow rates. The results indicate the successful performance of the LFCS with significant improvements over existing LFCS devices, facing the possibility of using the system for biological applications such as generating distinct perfusion modes in cell culture, novel digital microfluidics. Moreover, the integration capabilities and the reproducible fabrication method enable straightforward transition from prototype to product in a way that is lean, cost-effective and with reduced risk.

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Characterization of syringe-pump-driven versus pressure-driven microfluidic flows

Cited by 28 publications

References 16 publications

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation

Highly integrated polymeric microliquid flow controller for droplet microfluidics

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