Characterization of Surface Coats of Bacterial Spores with Atomic Force Microscopy and Wavelets

Sun, Wei; Romagnoli, José A.; Palazoğlu, Ahmet; Stroeve, Pieter

doi:10.1021/ie101153y

Cited by 3 publications

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the procedure of wavelets has increasingly been used for the analysis of bacterial genomes, such as wavelet packet analysis of amino acid chain sequences in the proteins of mesophile and thermophile bacteria 3 , comparative genomics via wavelet analysis for closely related bacteria 4 , discovery functional genetic material expression patterns in the metabolic pathways of Escherichia coli using wavelets transforms 5 , wavelet analysis to rapidly determine the characteristic morphology of the spore coat of bacteria 6 , and the existence of wavelet symmetries in Archaea DNA 7 . In a previous study, the authors bearing in mind the sequences of the MTB genome showed that the clustering analysis using the energy (variance) obtained at each decomposition level employing the discrete non-decimated wavelet transform (NDWT) was essential to verify the similarity of the sequences 8 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genome similarities using a wavelet-domain approach

Ferreira

Sáfadi

Ferreira

2020

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Tuberculosis is listed among the top 10 causes of deaths worldwide. The resistant strains causing this disease have been considered to be responsible for public health emergencies and health security threats. As stated by the World Health Organization (WHO), around 558,000 different cases coupled with resistance to rifampicin (the most operative first-line drug) have been estimated to date. Therefore, in order to detect the resistant strains using the genomes of Mycobacterium tuberculosis (MTB), we propose a new methodology for the analysis of genomic similarities that associate the different levels of decomposition of the genome (discrete non-decimated wavelet transform) and the Hurst exponent. Methods: The signals corresponding to the ten analyzed sequences were obtained by assessing GC content, and then these signals were decomposed using the discrete non-decimated wavelet transform along with the Daubechies wavelet with four null moments at five levels of decomposition. The Hurst exponent was calculated at each decomposition level using five different methods. The cluster analysis was performed using the results obtained for the Hurst exponent. Results: The aggregated variance, differenced aggregated variance, and aggregated absolute value methods presented the formation of three groups, whereas the Peng and R/S methods presented the formation of two groups. The aggregated variance method exhibited the best results with respect to the group formation between similar strains. Conclusion: The evaluation of Hurst exponent associated with discrete non-decimated wavelet transform can be used as a measure of similarity between genome sequences, thus leading to a refinement in the analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of genome similarities using a wavelet-domain approach

Ferreira

Sáfadi

Ferreira

2020

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

Bacillus thuringiensis as a surrogate for Bacillus anthracis in aerosol research

Tufts

Calfee

Lee

et al. 2013

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Characterization of candidate surrogate spores prior to experimental use is critical to confirm that the surrogate characteristics are as closely similar as possible to those of the pathogenic agent of interest. This review compares the physical properties inherent to spores of Bacillus anthracis (Ba) and Bacillus thuringiensis (Bt) that impact their movement in air and interaction with surfaces, including size, shape, density, surface morphology, structure and hydrophobicity. Also evaluated is the impact of irradiation on the physical properties of both Bacillus species. Many physical features of Bt and Ba have been found to be similar and, while Bt is considered typically non-pathogenic, it is in the B. cereus group, as is Ba. When cultured and sporulated under similar conditions, both microorganisms share a similar cylindrical pellet shape, an aerodynamic diameter of approximately 1 μm (in the respirable size range), have an exosporium with a hairy nap, and have higher relative hydrophobicities than other Bacillus species. While spore size, morphology, and other physical properties can vary among strains of the same species, the variations can be due to growth/sporulation conditions and may, therefore, be controlled. Growth and sporulation conditions are likely among the most important factors that influence the representativeness of one species, or preparation, to another. All Bt spores may, therefore, not be representative of all Ba spores. Irradiated spores do not appear to be a good surrogate to predict the behavior of non-irradiated spores due to structural damage caused by the irradiation. While the use of Bt as a surrogate for Ba in aerosol testing appears to be well supported, this review does not attempt to narrow selection between Bt strains. Comparative studies should be performed to test the hypothesis that viable Ba and Bt spores will behave similarly when suspended in the air (as an aerosol) and to compare the known microscale characteristics versus the macroscale response.

show abstract

Characterization of Bacteria–Biomaterial Interactions, from a Single Cell to Biofilms

Abu-Lail

Beyenal

2013

Characterization of Biomaterials

View full text Add to dashboard Cite

Characterization of Surface Coats of Bacterial Spores with Atomic Force Microscopy and Wavelets

Cited by 3 publications

References 42 publications

Evaluation of genome similarities using a wavelet-domain approach

Evaluation of genome similarities using a wavelet-domain approach

Bacillus thuringiensis as a surrogate for Bacillus anthracis in aerosol research

Characterization of Bacteria–Biomaterial Interactions, from a Single Cell to Biofilms

Contact Info

Product

Resources

About