Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness

Liu, Dong; Liu, Zengshan; Hu, Pan; Cai, Ling; Fu, Bao‐Quan; Li, Yansong; Lu, Shiying; Liu, Nannan; Ma, Xiaolong; Chi, Dan; Chang, Jiang; Shui, Yi-Ming; Li, Zhaohui; Ahmad, Waqas; Ren, Hong-Lin

doi:10.1016/j.vetmic.2016.02.018

Cited by 46 publications

(41 citation statements)

References 48 publications

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“…Fitness was investigated by using growth curve assay and in vitro competition experiments (Liu, D. et al, 2016). Three isolates (GZ1, GZ2, and GZ3) cultured overnight in LB broth were diluted to an OD 600 of 0.01 and grew at 37°C with vigorous aeration (200 rpm).…”

Section: Methodsmentioning

confidence: 99%

“…Each isolate was tested three times. The competitive index (CI) was determined as follows: CI = (GZ3/GZ2)/(Inoculated GZ3/Inoculated GZ2) as previously described (Liu, D. et al, 2016). …”

Section: Methodsmentioning

confidence: 99%

“…To verify the association between the resistance plasmids and the fitness, growth kinetics of the transconjugants and the recipient E. coli J53 were investigated (Liu, D. et al, 2016). The strains cultured overnight in LB broth were diluted to an OD 600 of 0.01 and grew at 37°C with vigorous aeration (200 rpm).…”

Section: Methodsmentioning

confidence: 99%

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Decreased Fitness and Virulence in ST10 Escherichia coli Harboring blaNDM-5 and mcr-1 against a ST4981 Strain with blaNDM-5

Zhang

Liao

Gao

et al. 2017

Front. Cell. Infect. Microbiol.

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Although coexistence of blaNDM-5 and mcr-1 in Escherichia coli has been reported, little is known about the fitness and virulence of such strains. Three carbapenem-resistant Escherichia coli (GZ1, GZ2, and GZ3) successively isolated from one patient in 2015 were investigated for microbiological fitness and virulence. GZ1 and GZ2 were also resistant to colistin. To verify the association between plasmids and fitness, growth kinetics of the transconjugants were performed. We also analyzed genomic sequences of GZ2 and GZ3 using PacBio sequencing. GZ1 and GZ2 (ST10) co-harbored blaNDM-5 and mcr-1, while GZ3 (ST4981) carried only blaNDM-5. GZ3 demonstrated significantly more rapid growth (P < 0.001) and overgrew GZ2 with a competitive index of 1.0157 (4 h) and 2.5207 (24 h). Increased resistance to serum killing and mice mortality was also identified in GZ3. While GZ2 had four plasmids (IncI2, IncX3, IncHI2, IncFII), GZ3 possessed one plasmid (IncFII). The genetic contexts of blaNDM-5 in GZ2 and GZ3 were identical but inserted into different backbones, IncX3 (102,512 bp) and IncFII (91,451 bp), respectively. The growth was not statistically different between the transconjugants with mcr-1 or blaNDM-5 plasmid and recipient (P = 0.6238). Whole genome sequence analysis revealed that 28 virulence genes were specific to GZ3, potentially contributing to increased virulence of GZ3. Decreased fitness and virulence in a mcr-1 and blaNDM-5 co-harboring ST10 E. coli was found alongside a ST4981 strain with only blaNDM-5. Acquisition of mcr-1 or blaNDM-5 plasmid did not lead to considerable fitness costs, indicating the potential for dissemination of mcr-1 and blaNDM-5 in Enterobacteriaceae.

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Section: Methodsmentioning

confidence: 99%

“…Each isolate was tested three times. The competitive index (CI) was determined as follows: CI = (GZ3/GZ2)/(Inoculated GZ3/Inoculated GZ2) as previously described (Liu, D. et al, 2016). …”

Section: Methodsmentioning

confidence: 99%

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Decreased Fitness and Virulence in ST10 Escherichia coli Harboring blaNDM-5 and mcr-1 against a ST4981 Strain with blaNDM-5

Zhang

Liao

Gao

et al. 2017

Front. Cell. Infect. Microbiol.

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“…The recA mutant shows significantly reduced survival within macrophages and decreases lethality in a mouse model of systemic infection (Aranda et al, 2011). The surface antigen protein 1 (SurA1) plays an important role in fitness and virulence of A. baumannii (Liu D. et al, 2016). Serum resistance of the surA1 mutant significantly decreases compared with that of the wild-type strain CCGGD201101.…”

Section: Acinetobacter Baumannii Virulence Factors and Pathogenesismentioning

confidence: 99%

“…Serum resistance of the surA1 mutant significantly decreases compared with that of the wild-type strain CCGGD201101. In the G. mellonella insect model, a surA1 mutant strain exhibits a lower survival rate and decreased dissemination (Liu D. et al, 2016). …”

Section: Acinetobacter Baumannii Virulence Factors and Pathogenesismentioning

confidence: 99%

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Lee

Park

et al. 2017

Front. Cell. Infect. Microbiol.

654

548

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Acinetobacter baumannii is undoubtedly one of the most successful pathogens responsible for hospital-acquired nosocomial infections in the modern healthcare system. Due to the prevalence of infections and outbreaks caused by multi-drug resistant A. baumannii, few antibiotics are effective for treating infections caused by this pathogen. To overcome this problem, knowledge of the pathogenesis and antibiotic resistance mechanisms of A. baumannii is important. In this review, we summarize current studies on the virulence factors that contribute to A. baumannii pathogenesis, including porins, capsular polysaccharides, lipopolysaccharides, phospholipases, outer membrane vesicles, metal acquisition systems, and protein secretion systems. Mechanisms of antibiotic resistance of this organism, including acquirement of β-lactamases, up-regulation of multidrug efflux pumps, modification of aminoglycosides, permeability defects, and alteration of target sites, are also discussed. Lastly, novel prospective treatment options for infections caused by multi-drug resistant A. baumannii are summarized.

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Genetic mechanisms of antibiotic resistance and virulence in Acinetobacter baumannii: background, challenges and future prospects

Chakravarty

2020

Mol Biol Rep

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Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness

Cited by 46 publications

References 48 publications

Decreased Fitness and Virulence in ST10 Escherichia coli Harboring blaNDM-5 and mcr-1 against a ST4981 Strain with blaNDM-5

Decreased Fitness and Virulence in ST10 Escherichia coli Harboring blaNDM-5 and mcr-1 against a ST4981 Strain with blaNDM-5

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Genetic mechanisms of antibiotic resistance and virulence in Acinetobacter baumannii: background, challenges and future prospects

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