Characterization of sulfated polysaccharide from Laurencia obtusa and its apoptotic, gastroprotective and antioxidant activities

Lajili, Sirine; Ammar, Hiba Hadj; Mzoughi, Zeineb; Amor, Haifa Bel Haj; Muller, Christian D.; Majdoub, Hatem; Bouraoui, Abderrahman

doi:10.1016/j.ijbiomac.2018.12.089

Cited by 33 publications

(23 citation statements)

References 70 publications

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“…The extraction yield of JSP was around 4.55% (w/w) and close to those obtained by Zeid et al [35], Navarro et al [2], Maciel et al [36] and Chiovitti et al [37] for the extraction of galactans from the red seaweeds Pterocladia capillacea (6.46%), L. heterocladum (6.20%), Gracilaria birdiae (6.50%) and Sarconema filiforme (6.0%). This extraction yield was higher than those obtained from J. rubens (<1.4%), C. officinalis (0.3%) and B. orbigniana (0.25%) [3,18], but remained much lower than those obtained by Lajili et al [38], Fenoradosoa et al [16] and Chattopadhyay et al [39] for the red algae Laurencia obtusa (12.80%), Halymenia durvillei (~15-18%) and Grateloupia indica (13.0%). Overall, the extraction yield of sulfated galactans from Corallinales are probably lower due to their strong calcareous covers.…”

Section: Extraction Yield and Biochemical Compositioncontrasting

confidence: 54%

“…The absorption signal around 1609 cm −1 was ascribed to (-COO-) carboxylate groups, whereas those at 1417 cm −1 suggested the presence of (C=O) ester carbonyl groups of acid residues, confirming the weak electrolytic character of JSP (5.63%) [41]. FTIR spectra showed characteristic bands (1236, 1073, 1023, 934 and 875 cm −1 ) of agar-like polysaccharide (agarans structure) [1,37,38].…”

Section: Atr-ftir Spectroscopymentioning

confidence: 86%

“…This desulfation step, with a yield of 46.35% (w/w), was also confirmed by colorimetric assays (2.08%). The absorption band observed at 1236 cm −1 could be attributed to asymmetric O=S=O stretching vibration of sulfate ester [30,38,41]. The peaks near to 1073 and 1148 cm −1 were assigned to C-O-C and C-O glycosidic band vibrations, which suggested the presence of carbohydrates.…”

Section: Atr-ftir Spectroscopymentioning

confidence: 93%

“…FTIR spectra showed characteristic bands (1236, 1073, 1023, 934 and 875 cm −1 ) of agar-like polysaccharide (agarans structure) [1,37,38]. The absorption band observed at 1236 cm −1 could be attributed to asymmetric O=S=O stretching vibration of sulfate ester [30,38,41]. The peaks near to 1073 and 1148 cm −1 were assigned to C-O-C and C-O glycosidic band vibrations, which suggested the presence of carbohydrates.…”

Section: Atr-ftir Spectroscopymentioning

confidence: 97%

“…Sci. 2020, 10, 1655 6 of 18 of sulfated galactans is largely dependent on the algal species, environmental factors and extraction procedures [16,38].…”

Section: Extraction Yield and Biochemical Compositionmentioning

confidence: 99%

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Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

et al. 2020

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A novel sulfated xylogalactan-rich fraction (JSP for J. adhaerens Sulfated Polysaccharide) was extracted from the red Tunisian seaweed Jania adhaerens. JSP was purified using an alcoholic precipitation process and characterized by Attenuated Total Reflectance-Fourier-transform infrared spectroscopy (ATR-FTIR), high-pressure size exclusion chromatography (HPSEC) with a multi-angle laser light scattering (MALLS), gas chromatography coupled to mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR, 1D and 2D). JSP was then evaluated regarding its physicochemical and rheological properties. Results showed that JSP was mainly composed of an agar-like xylogalactan sharing the general characteristics of corallinans. The structure of JSP was mainly composed of agaran disaccharidic repeating units (→3)-β-d-Galp-(1,4)-α-l-Galp-(1→) n and (→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→) n , mainly substituted on O-6 of (1,3)-β-d-Galp residues by β-xylosyl side chains, and less with sulfate or methoxy groups. (1,4)-α-l-Galp residues were also substituted by methoxy and/or sulfate groups in the O-2 and O-3 positions. Mass-average and number-average molecular masses (M w ) and (M n ), intrinsic viscosity ([η]) and hydrodynamic radius (R h ) for JSP were, respectively, 8.0 × 10 5 g/mol, 1.0 × 10 5 g/mol, 76 mL/g and 16.8 nm, showing a flexible random coil conformation in solution. The critical overlap concentration C* of JSP was evaluated at 7.5 g/L using the Williamson model. In the semi-diluted regime, JSP solutions displayed a shear-thinning behavior with a great viscoelasticity character influenced by temperature and monovalent salts. The flow characteristics of JSP were described by the Ostwald model. always belongs to the d-series, while the former (α-Gal) can occur as d-(carrageenans) or l-(agarans) series [4][5][6][7]. The main backbone can be substituted by pyruvic acid ketals, sulfate, O-glycosyl and/or methyl groups and also by side chains, giving rise to great structural changes along the galactan chain. These structural changes affect the physicochemical and rheological behaviors of these polymers. So far, it has been reported that sulfated galactans exhibit numerous biological properties, including antioxidant, anticoagulant, antinociceptive, immunomodulatory, anticancer, gastroprotective, anti-inflammatory, antithrombotic and antiproliferative [1,[6][7][8][9][10][11][12][13][14]. Marine seaweed (Phaeophyceae and Rhodophyceae) cell wall polysaccharides also hold great promise as a raw material for feed, food, and biofuel production. Through metabolic engineering, seaweeds (micro-and macroalgae) are attracting growing attention as an alternative source of biofuel by converting the seaweed biomass and, in particular, the cell wall, which is rich in carbohydrates, into liquid biofuels [15].Galactans are currently used as thickening, gelling, and stabilizing agents in food, cosmetic, and pharmaceutical fields [1,10-16]. However, sulfated galactans produced by Corallinales are less studied, probably because ...

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Section: Extraction Yield and Biochemical Compositioncontrasting

confidence: 54%

Section: Atr-ftir Spectroscopymentioning

confidence: 86%

Section: Atr-ftir Spectroscopymentioning

confidence: 93%

Section: Atr-ftir Spectroscopymentioning

confidence: 97%

“…Sci. 2020, 10, 1655 6 of 18 of sulfated galactans is largely dependent on the algal species, environmental factors and extraction procedures [16,38].…”

Section: Extraction Yield and Biochemical Compositionmentioning

confidence: 99%

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Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

et al. 2020

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Gastroprotective polysaccharide from natural sources: Review on structure, mechanism, and structure–activity relationship

Wang

Yin

et al. 2022

Food Frontiers

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Polysaccharides from natural sources have the potentials in being used as substitutes of chemosynthetic drugs for gastroprotection because of its safety and efficacy.For giving a better understanding of gastroprotective polysaccharides, the research progress on preparation, structure, bioactivity, and their action mechanism is comprehensively summarized in this review. Moreover, the structure-activity relationship of gastroprotective polysaccharides is discussed. Accumulating evidence has indicated that natural polysaccharides, which were widely prepared by water extraction and column chromatography purifications, exhibited gastroprotective effects in vitro and in vivo. The action mechanism might be related to gastric secretions, promotion of gastric defensive factor releases, antioxidation, anti-inflammatory, antiapoptosis, and facilitation of proliferation. Phenolic compounds, molecular weight and conformation, monosaccharide composition, backbone structure and side chain, and functional group have great influences on the gastroprotective activities of polysaccharides. This review gives comprehensive guidance to the exploitation and application of natural polysaccharides in food and other industries for gastroprotection.

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Modification, Structural Characterizations, and Biological Activities of Sulfated Polysaccharides: A Review

Hao,

Dai,

Tan

et al. 2023

Starch Stärke

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Polysaccharides are another important class of bioactive macromolecules in organisms, in addition to proteins and nucleic acids. Numerous studies have confirmed that polysaccharides have various biological activities, including anti‐inflammatory, antiviral, antioxidant, immunomodulatory, anticancer, and other activities, which have attracted the attention of researchers in the biomedical field. In the past decade, increasing researches have found that sulfation modification can improve many physicochemical properties of polysaccharides, significantly enhance their original biological activities, and even generate new activity. Hence, sulfated polysaccharides have attracted more and more attention. A systematic review of the latest research progress and future development prospects of sulfated polysaccharides is very essential to better understand them. Hence, the study has systematically summarized current knowledge about synthesis, structural characteristics, biological activities, and potential molecular mechanisms of sulfated polysaccharides. This review provides some valuable insights and important guidance for the further study of sulfated polysaccharides.

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Characterization of sulfated polysaccharide from Laurencia obtusa and its apoptotic, gastroprotective and antioxidant activities

Cited by 33 publications

References 70 publications

Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

Gastroprotective polysaccharide from natural sources: Review on structure, mechanism, and structure–activity relationship

Modification, Structural Characterizations, and Biological Activities of Sulfated Polysaccharides: A Review

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