Characterization of structure, physico-chemical properties and diffusion behavior of Ca-Alginate gel beads prepared by different gelation methods

Puguan, John Marc C.; Yu, Xiaoming; Kim, Hern

doi:10.1016/j.jcis.2014.06.048

Cited by 61 publications

(36 citation statements)

References 18 publications

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“…Pectin [33] Alginate beads [35] Whey protein gels, aerated [57] Transmission electron microscopy (TEM) Fluorescence correlation spectroscopy Xanthan gels [55 ] Alginate gels [22,29] m-Particle image velocimetry b-Lactoglobulin [20 ] Internal methods which use small particles, belong to the group of techniques summarized as (nano)particle diffusometry. They require small testing micro-scale or nanoscale particles, which move through the gel by molecular self-diffusion and have to be well adapted to the purpose [55 ]:…”

Section: Internal Methods Substrate Referencesmentioning

confidence: 99%

Methods for investigation of diffusion processes and biopolymer physics in food gels

Einhorn-Stoll

Drusch

2015

Current Opinion in Food Science

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Section: Internal Methods Substrate Referencesmentioning

confidence: 99%

Methods for investigation of diffusion processes and biopolymer physics in food gels

Einhorn-Stoll

Drusch

2015

Current Opinion in Food Science

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“…There are, however, contradictory reports on the swelling behaviour of calcium alginate microhydrogels in acidic pH. Some researchers have observed a partial shrinkage for particles in these conditions [46]. Ouwerx et al [47] explained that at pH values lower than 4 carboxyl groups of alginate become protonated and the network may undergo a volume shrinkage as a result of decreased electrostatic repulsions between undissociated groups.…”

Section: Swelling Of Particlesmentioning

confidence: 99%

Electrospray-assisted encapsulation of caffeine in alginate microhydrogels

Nikoo¹,

Kadkhodaee²,

Ghorani³

et al. 2018

International Journal of Biological Macromolecules

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One of the major challenges with microencapsulation and delivery of low molecular weight bioactive compounds is their diffusional loss during storage and process conditions as well as under gastric conditions. In an attempt to slow down the release rate of core material, electrospray fabricated calcium alginate microhydrogels were coated with low molecular weight and high molecular weight chitosans. Caffeine as a hydrophilic model compound was used due to its several advantages on human behavior especially increasing consciousness. Mathematical modeling of the caffeine release by fitting the data with Korsmeyer-Peppas model showed that Fick's diffusion law could be the prevalent mechanism of the release. Electrostatic interaction between alginate and chitosan (particularly in the presence of 1% low molecular weight chitosan) provided an effective barrier against caffeine release and significantly reduced swelling of particles compared to control samples. The results of this study demonstrated that calcium alginate microhydrogels coated by chitosan could be used for encapsulation of low molecular compounds. However, more complementary research must be done in this field. In addition, electrospray, by producing monodisperse particles, would be as an alternative method for fabrication of microparticles based on natural polymers.

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“…In conjunction, indirect diffusivity estimations using pore size characterization by various scanning probe microscopy (SPM) methods have been documented [14,15,16]. Other methods include spectrophotometry [17,18,19,20], size exclusion chromatography [8,21], mechanical release tests [22,23] and fluorescence microscopy [23,24,25]. Regardless of the diffusivity characterization method, the following are a sub-set of factors affecting cross-link homogeneity, size and density, gel isotropy and, thus, membrane diffusivity [13,19,20]: (1) the β- d -mannuronic (M) to α- l -guluronic (G) ratio, the distribution of each monomer within the chain, and alginate molecular weight (MW); (2) the kinetics of gelation, specifically external gelation (chelation) vs. internal gelation.…”

Section: Introductionmentioning

confidence: 99%

Fickian-Based Empirical Approach for Diffusivity Determination in Hollow Alginate-Based Microfibers Using 2D Fluorescence Microscopy and Comparison with Theoretical Predictions

Mobed-Miremadi

Djomehri

Keralapura³

et al. 2014

Materials

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Hollow alginate microfibers (od = 1.3 mm, id = 0.9 mm, th = 400 µm, L = 3.5 cm) comprised of 2% (w/v) medium molecular weight alginate cross-linked with 0.9 M CaCl2 were fabricated to model outward diffusion capture by 2D fluorescent microscopy. A two-fold comparison of diffusivity determination based on real-time diffusion of Fluorescein isothiocyanate molecular weight (FITC MW) markers was conducted using a proposed Fickian-based approach in conjunction with a previously established numerical model developed based on spectrophotometric data. Computed empirical/numerical (Dempiricial/Dnumerical) diffusivities characterized by small standard deviations for the 4-, 70- and 500-kDa markers expressed in m2/s are (1.06 × 10−9 ± 1.96 × 10−10)/(2.03 × 10−11), (5.89 × 10−11 ± 2.83 × 10−12)/(4.6 × 10−12) and (4.89 × 10−12 ± 3.94 × 10−13)/(1.27 × 10−12), respectively, with the discrimination between the computation techniques narrowing down as a function of MW. The use of the numerical approach is recommended for fluorescence-based measurements as the standard computational method for effective diffusivity determination until capture rates (minimum 12 fps for the 4-kDa marker) and the use of linear instead of polynomial interpolating functions to model temporal intensity gradients have been proven to minimize the extent of systematic errors associated with the proposed empirical method.

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Characterization of structure, physico-chemical properties and diffusion behavior of Ca-Alginate gel beads prepared by different gelation methods

Cited by 61 publications

References 18 publications

Methods for investigation of diffusion processes and biopolymer physics in food gels

Methods for investigation of diffusion processes and biopolymer physics in food gels

Electrospray-assisted encapsulation of caffeine in alginate microhydrogels

Fickian-Based Empirical Approach for Diffusivity Determination in Hollow Alginate-Based Microfibers Using 2D Fluorescence Microscopy and Comparison with Theoretical Predictions

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