Characterization of stress response involved in chicken myopathy

Kang, Seong Wook; Kidd, M. T.; Kadhim, Hakeem J.; Shouse, Stephanie; Orlowski, Sara; Hiltz, J.Z.; Anthony, N. B.; Kuenzel, Wayne J.; Kong, Byung-Whi

doi:10.1016/j.ygcen.2020.113526

Cited by 10 publications

(13 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, mammalian studies indicate that liver disease might be implicated as a conjoint pathological mechanism underlying several metabolic disorders including neuromuscular disease (Pearce and Grant, 2010;David et al, 2014). Recent studies have likewise assessed the alterations of transcriptome and stress response genes in both liver and muscle of WB myopathic birds and revealed that the etiology of this myopathy is not limited to muscle, but is a systemic pathology (Kang et al, 2020;Phillips et al, 2020). Therefore, this study was conducted to investigate the histological and biochemical status and to depict the possible mechanistic changes involved in the liver of myopathic birds for better understanding of the molecular basis of the underlying pathological process and thereby promoting the healthy production of broiler chickens.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, lung histopathology of WB myopathic birds exhibited occasional localized multifocal lymphoplasmacytic phlebitis and more foci of chondro-osseous metaplasia compared with the unaffected birds (Lake et al, 2020). WB myopathy increased stress hormone corticosterone levels in plasma and altered expression patterns of stress responserelated genes in the liver (Kang et al, 2020). Assessment of potential systemic physiological disparities accompanying WB myopathy might contribute to a profound understanding of its etiology.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

et al. 2021

View full text Add to dashboard Cite

Wooden breast (WB) syndrome has emerged as a global myopathy in modern commercial broiler chickens, mainly affecting the pectoralis major muscle. Recent evidence suggests that WB myopathy is a systemic disease, which might be accompanied by other physiological disparities and metabolic changes. This study was conducted to systemically investigate the potential physiological changes in liver tissues as well as the possible mechanisms involved to enhance the understanding of the etiology. A total of 93 market-age Arbor Acres male broiler chickens were sampled and categorized into control (CON) and WB groups based on the evaluation of myopathic lesions. Liver samples were collected (n = 10 in each group) for histopathological evaluation and biochemical analyses. Results indicated that WB birds exhibited significantly higher plasma aspartate amino transferase, alkaline phosphatase, and gamma glutamyl transpeptidase activities. Histopathological changes in hydropic/fatty degeneration, inflammatory cell infiltration, intrahepatic hemorrhages, elevated myeloperoxidase activity, and overproduction of nitric oxide were observed in WB liver compared with CON, suggesting the occurrence of liver injury in birds affected by WB myopathy. The WB group showed increased levels of reactive oxygen species, oxidative products, as well as enhanced antioxidant capacities in the liver. These changes were associated with impaired mitochondria morphology and mitochondrial dysfunction. WB myopathy also induced mitochondria-mediated hepatic apoptosis by upregulating levels of caspases 3 and 9, altering the expressions of apoptotic B-cell lymphoma-2 family regulators, as well as increasing the release of cytochrome c. The activation of nuclear factor kappa-light-chain-enhancer of activated B cell signaling enhanced the mRNA expression of downstream inflammatory mediators, contributing to the production of inflammatory cytokines in WB liver. Combined, these findings suggest that hepatic disorders may be conjoined with WB myopathy in broiler chickens and indicating systemic physiological disparities, and other metabolic changes accompanying this myopathy need further assessment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Damaged muscle fibers were replaced by collagen and adipose tissue and exhibited altered sarcomeric structure and functions ( Papah et al, 2017 ; Velleman et al, 2017 ; Liu et al, 2020 ). Metabolic processes, ion homeostasis, as well as oxidative stress response mechanisms can be profoundly disrupted ( Mutryn et al, 2015 ; Abasht et al, 2016 ; Kang et al, 2020 ). Soglia et al (2020) recently demonstrated an increase in vimentin (VIM) and desmin (DES) in WB muscles compared with those of normal counterparts, suggesting the occurrence of intensive regenerative processes within these muscles.…”

Section: Introductionmentioning

confidence: 99%

Insights Into Transcriptome Profiles Associated With Wooden Breast Myopathy in Broilers Slaughtered at the Age of 6 or 7 Weeks

et al. 2021

View full text Add to dashboard Cite

Transcriptomes associated with wooden breast (WB) were characterized in broilers at two different market ages. Breasts (Pectoralis major) were collected, 20-min postmortem, from male Ross 308 broilers slaughtered at 6 and 7 weeks of age. The breasts were classified as “non-WB” or “WB” based on palpation hardness scoring (non-WB = no abnormal hardness, WB = consistently hardened). Total RNA was isolated from 16 samples (n = 3 for 6 week non-WB, n = 3 for 6 week WB; n = 5 for 7 week non-WB, n = 5 for 7 week WB). Transcriptome was profiled using a chicken gene expression microarray with one-color hybridization technique, and compared between non-WB and WB samples of the same age. Among 6 week broilers, 910 transcripts were differentially expressed (DE) (false discovery rate, FDR < 0.05). Pathway analysis underlined metabolisms of glucose and lipids along with gap junctions, tight junction, and focal adhesion (FA) signaling as the top enriched pathways. For the 7 week broilers, 1,195 transcripts were identified (FDR < 0.05) with regulation of actin cytoskeleton, mitogen-activated protein kinase (MAPK) signaling, protein processing in endoplasmic reticulum and FA signaling highlighted as the enriched affected pathways. Absolute transcript levels of eight genes (actinin-1 – ACTN1, integrin-linked kinase – ILK, integrin subunit alpha 8 – ITGA8, integrin subunit beta 5 – ITGB5, protein tyrosine kinase 2 – PTK2, paxillin – PXN, talin 1 – TLN1, and vinculin – VCL) of FA signaling pathway were further elucidated using a droplet digital polymerase chain reaction. The results indicated that, in 6 week broilers, ITGA8 abundance in WB was greater than that of non-WB samples (p < 0.05). Concerning 7 week broilers, greater absolute levels of ACTN1, ILK, ITGA8, and TLN1, accompanied with a reduced ITGB5 were found in WB compared with non-WB (p < 0.05). Transcriptional modification of FA signaling underlined the potential of disrupted cell-cell communication that may incite aberrant molecular events in association with development of WB myopathy.

show abstract

“…inflammation, response to reactive oxygen species and blood vessels morphogenesis 5,[10][11][12] . These functional categories are often involved in case of compromised blood and oxygen supply, suggesting that tissues affected by modern myopathies could be characterized by hypoxic conditions 5,10,13 .…”

mentioning

confidence: 99%

Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies

Bordini

Zappaterra

Soglia

et al. 2021

Sci Rep

View full text Add to dashboard Cite

In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform a Weighted Gene Co-expression Network Analysis (WGCNA) using the gene expression profile and meat quality parameters of Pectoralis major muscles analysed in our previous study. Among the 212 modules identified by WGCNA, the red, darkred, midnightblue and paleturquoise4 modules were chosen for subsequent analysis. Functional analysis evidenced pathways involved in extracellular matrix (ECM) organization, collagen metabolism, cellular signaling and unfolded protein response. The hub gene analysis showed several genes coding for ECM components as the most interconnected nodes in the gene network (e.g. COL4A1, COL4A2, LAMA2, LAMA4, FBLN5 and FBN1). In this regard, this study suggests that alterations in ECM composition could somehow activate the cascade of biological reactions that result in the growth-related myopathies onset, and the involvement of Collagen IV alterations in activating the endoplasmic reticulum (ER) stress response may be hypothesized. Therefore, our findings provide further and innovative knowledge concerning the molecular mechanisms related to the breast abnormalities occurrence in modern broilers.

show abstract

Characterization of stress response involved in chicken myopathy

Cited by 10 publications

References 84 publications

Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

Insights Into Transcriptome Profiles Associated With Wooden Breast Myopathy in Broilers Slaughtered at the Age of 6 or 7 Weeks

Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies

Contact Info

Product

Resources

About