Characterization of Streptococcus suis capsular type 2 haemolysin

Gottschalk, Marcelo; Lacouture, Sonia; Dubreuil, J. Daniel

doi:10.1099/00221287-141-1-189

Cited by 101 publications

(116 citation statements)

References 22 publications

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“…More recently, a thiol-activated haemolysin, the Suilysin, was described as a potential virulence factor of S. suis [7,12,18]. The production of this S. suis haemolysin was evidenced in strains collected from diseased pigs, but its role has not been determined [19,27].…”

Section: Introductionmentioning

confidence: 99%

Production of Muraminidase-Released Protein (MRP), Extracellular Factor (EF) and Suilysin by field isolates of Streptococcus suis capsular types 2, 1/2, 9, 7 and 3 isolated from swine in France

Berthelot-Hérault

Morvan²,

Keribin³

et al. 2000

Vet. Res.

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-A total of 323 isolates of Streptococcus suis recovered from diseased or healthy pigs in France were serotyped. The presence of virulence-related proteins, Muraminidase-Released Protein (MRP), Extracellular Factor (EF) and Suilysin was also studied in 122 isolates of capsular types 2, 1/2, 9, 7 and 3 to evaluate their implication in virulence of S. suis. Capsular types 2, 1/2, 9, 7 and 3 were the most frequently detected (93%), with 69% for the capsular type 2 alone. Capsular types 2, 1/2, 9, 7, 3, 1, 4, 8, 18, 10 and 12 were isolated from diseased pigs, whereas types 2, 7, 9, 1/2, and 3 originated from the nasal cavities or tonsils of healthy animals. Most of the S. suis type 2 isolates recovered from diseased pigs carried MRP+ EF-Suilysin-(46%) or MRP+ EF+ Suilysin+ (28%) phenotypes. The MRP+ EF-Suilysin-phenotype was also detected in 67% of S. suis type 2 strains isolated from healthy pigs. The production of the virulence-related proteins was less frequently found in S. suis types 1/2, 9, 7 and 3 recovered either from diseased or healthy pigs. In this study, all the capsular type 1/2 strains were MRP+ EF-Suilysin-and all the S. suis type 7 harboured an MRP-EF-Suilysin-phenotype. The MRP-EF-Suilysin-phenotype was found in S. suis types 2, 3, 7 and 9 isolated from septicaemia, meningitis, pneumonia, and pleurisy. These results suggest that the presence of these proteins should not be used as a single condition for classifying the virulence of a field isolate in France. Streptococcus suis / capsular type / virulence / swine

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Section: Introductionmentioning

confidence: 99%

Production of Muraminidase-Released Protein (MRP), Extracellular Factor (EF) and Suilysin by field isolates of Streptococcus suis capsular types 2, 1/2, 9, 7 and 3 isolated from swine in France

Berthelot-Hérault

Morvan²,

Keribin³

et al. 2000

Vet. Res.

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“…nos AF106927, Z95920 and A57222) and of these, only a few are putatively involved in virulence (Smith et al, , 1993(Smith et al, , 1999Jacobs et al, 1994 ;Gottschalk et al, 1995 ;Segers et al, 1998). Previously, putative virulence factors have been identified after growth of bacteria in standard laboratory media.…”

Section: Abbreviation : Ivet In Vivo Expression Technologymentioning

confidence: 99%

Environmentally regulated genes of Streptococcus suis: identification by the use of iron-restricted conditions in vitro and by experimental infection of piglets The GenBank accession numbers for the sequences determined in this work are AF302190–AF302207 (iri genes) and AF303226–303247 (ivs genes).

Smith¹,

Buijs²,

Vries³

et al. 2001

Microbiology

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The identification of environmentally regulated genes of Streptococcus suis by the use of iron-restricted conditions in vitro and by experimental infection of piglets is described. Eighteen unique iron-restriction-induced (iri) genes and 22 unique in-vivo-selected (ivs) genes of Strep. suis were found. None of the ivs genes was exclusively expressed in vivo. Four iri genes were identical to four clones selected in piglets. Two ivs genes were similar to genes for putative virulence factors. One of these ivs genes was identical to the epf gene of virulent Strep. suis serotype 2 strains and the other showed homology to a gene encoding a fibronectin-binding protein of Streptococcus gordonii. Two additional ivs genes showed homology to environmentally regulated genes previously identified by using an in vivo expression technology (IVET) selection system in other bacterial species. One of these showed similarity to the agrA gene of Staphylococcus aureus, a key locus involved in the regulation of numerous virulence proteins. The promoter selection system described in this paper has been successfully used for the identification of many environmentally regulated genes potentially involved in the pathogenesis of Strep. suis infections in piglets.

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“…Production of haemolysin was tested as described previously (Gottschalk et al, 1995). Production of MRP and EF was tested as described by Vecht et al (1991).…”

Section: Characterization Of Mutantsmentioning

confidence: 99%

“…Several virulence factor candidates have been proposed. These include the polysaccharide capsule, cell-wall and extracellular proteins, including muramidase-released protein (MRP), extracellular factor (EF) and adhesins, and a haemolysin (Gottschalk et al, 1995;Jacobs et al, 1994;Tikkanen et al, 1996;Vecht et al, 1991). Recent studies have shown that S. suis mutants which produce neither MRP nor EF are as virulent as the wild-type strain (Smith et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Streptococcus suis serotype 2 mutants deficient in capsular expression

Charland

Harel

Kobisch³

et al. 1998

Microbiology

141

143

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Streptococcus suis serotype 2 is responsible for a wide variety of porcine infections. In addition, it is considered a zoonotic agent. Knowledge about the virulence factors for this bacterium is limited but its polysaccharide capsule is thought to be one of the most important. Transposon mutagenesis with the self-conjugative transposon Tn916 was used to obtain acapsular mutants from the virulent 5. suis type 2 reference strain 5735. Clones were screened by colony-dot ELISA with a monoclonal antibody specific for a type 2 capsular epitope and clones that failed to react with the antibody were characterized. Two mutants, 2A and 79, having one and two Tn916 insertions respectively, were chosen for further characterization. Absence of capsule was confirmed by coagglutination, capillary precipitation and capsular reaction tests and by transmission electron microscopy. Absence of capsular polysaccharides correlated with increased hydrophobicity and phagocytosis by both murine macrophages and porcine monocytes compared to the wild-type strain. Furthermore, both mutants were shown to be avirulent in murine and pig models of infection. Finally, mutant 2A was readily eliminated from circulation in mice compared to the wild-type strain, which persisted more than 48 h in blood. Thus, isogenic mutants defective in capsule production demonstrate the importance of capsular polysaccharides as a virulence factor for 5. suis type 2.

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Characterization of Streptococcus suis capsular type 2 haemolysin

Cited by 101 publications

References 22 publications

Production of Muraminidase-Released Protein (MRP), Extracellular Factor (EF) and Suilysin by field isolates of Streptococcus suis capsular types 2, 1/2, 9, 7 and 3 isolated from swine in France

Production of Muraminidase-Released Protein (MRP), Extracellular Factor (EF) and Suilysin by field isolates of Streptococcus suis capsular types 2, 1/2, 9, 7 and 3 isolated from swine in France

Streptococcus suis serotype 2 mutants deficient in capsular expression

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