Characterization of strains of Weissella fabalis sp. nov. and Fructobacillus tropaeoli from spontaneous cocoa bean fermentations

Abstract

Six facultatively anaerobic, non-motile lactic acid bacteria were isolated from spontaneous cocoa bean fermentations carried out in Brazil, Ecuador and Malaysia. Phylogenetic analysis revealed that one of these strains, designated M75 T , isolated from a Brazilian cocoa bean fermentation, had the highest 16S rRNA gene sequence similarity towards Weissella fabaria LMG 24289 T (97.7 %), W. ghanensis LMG 24286 T (93.3 %) and W. beninensis LMG 25373 T (93.4 %). The remaining lactic acid bacteria isolates, represented by strain M622, showed the highest 16S rRNA gene sequence similarity towards the type strain of Fructobacillus tropaeoli (99.9 %), a recently described species isolated from a flower in South Africa. pheS gene sequence analysis indicated that the former strain represented a novel species, whereas pheS, rpoA and atpA gene sequence analysis indicated that the remaining five strains belonged to F. tropaeoli; these results were confirmed by DNA-DNA hybridization experiments towards their respective nearest phylogenetic neighbours. Additionally, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved successful for the identification of species of the genera Weissella and Fructobacillus and for the recognition of the novel species. We propose to classify strain M75 T (5LMG 26217 T 5CCUG 61472 T

The microbial diversity of an industrially produced lambic beer shares members of a traditionally produced one and reveals a core microbiota for lambic beer fermentation

“…All PCR assays were performed as described by Snauwaert et al (2013). Bacterial DNA was obtained via the protocol described by Niemann et al (1997), whereas yeast DNA was obtained using the protocol of Harju et al (2004).…”

Weissella uvarum sp. nov., isolated from wine grapes

“…Since then, several novel species of the genus Weissella have been described, most of which have been associated with nutrient-rich environments like fermented foods or food products (Weissella beninensis, Weissella ghanensis, Weissella fabalis, Weissella fabaria, Weissella koreensis, Weissella oryzae, Weissella thailandensis) (Tanasupawat et al, 2000;Lee et al, 2002;De Bruyne et al, 2008Padonou et al, 2010;Snauwaert et al, 2013;Tohno et al, 2013). A few species (Weissella ceti, Weissella diestrammenae and Weissella cibaria) have been isolated from human or animal sources (Björkroth et al, 2002;Vela et al, 2011;Oh et al, 2013), while Weissella soli was isolated from soil samples (Magnusson et al, 2002).…”

Bifidobacterium commune sp. nov. isolated from the bumble bee gut

“…The 16S rRNA gene sequence of strain LMG 28292 T was determined as previously described (Snauwaert et al 2013). The forward primer MO-157 (5 0 -CTGGT GAAGGAGGTCGCCAA-3 0 ) and the reverse primer MO-158 (5 0 -CCATATCCTGCAGCATAGCCTT-3 0 ) were used for hsp60 gene sequence analysis of the isolates LMG 28292 T , R-53560, R-53124 and LMG 28626 (Okamoto et al 2008).…”