Characterization of stenocephol from <i>Seriphidium stenocephalum</i> as potent HepG2 cell growth and glycogen phosphorylase inhibitor

Shafiq, Nusrat; Arshad, Uzma; Brogi, Simone; Rashid, Maryam; Rafiq, Naila; Parveen, Shagufta

doi:10.1080/14786419.2022.2117177

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…The crude methanolic extract was concentrated with the help of a rotary evaporator under reduced pressure at 50 °C. 23 As a result, a reddish-brown gummy mass was obtained. The extract was suspended in distilled water (1.0 L) and was fractionated into hexane (HXF) (40 g), ethyl acetate (EAF) (100 g), and methanol (MEF) (110 g) parts (Supporting Information, Scheme S1).…”

Section: Plant Collection Identification and Extractionmentioning

confidence: 97%

Plant-Based Bioactive Phthalates Derived from Hibiscus rosa-sinensis: As In Vitro and In Silico Enzyme Inhibition

Yasmin,

Nazli,

Shafiq

et al. 2023

ACS Omega

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Hibiscus rosa-sinensis is an attractive, ever-blossoming, and effortlessly available plant around the globe. The fabulous flowers of H. rosa-sinensis enjoy a significant status in folk medicine throughout the world and comprise a range of phyto constituents due to which this splendid flower owns numerous biological and pharmaceutical activities like antioxidant, antifungal, antimicrobial, anti-inflammatory, antipyretic, antidiabetic, and antifertility activity. Considering this, column chromatographic isolation of the phytoconstituents of ethyl acetate fraction of the flowers of H. rosa-sinensis was performed. A series of five phthalates including Di- n -octyl phthalate ( HR1 ), ditridecyl phthalate ( HR2 ), 1-allyl 2-ethyl phthalate ( HR3 ), diethyl phthalate ( HR4 ), and bis (6-methylheptyl) phthalate ( HR5 ) were isolated. The structures of the isolated phthalates were elucidated by gas chromatography–mass spectrometry, 1 H NMR, and 13 C NMR. In silico and in vitro antidiabetic and antioxidant potential and DFT studies of isolated phthalates were carried out. In our study, isolated ligands were explored as potent antidiabetic as well as antioxidant agents as they exhibited good binding affinity (in in vitro and in silico experiments) against all selected protein targets. Compounds HR1–HR5 showed that the binding affinity value ranged from −5.9 to −5.2 kcal/mol, −5.5 to −4.3 kcal/mol, and −5.0 to −4.1 kcal/mol for target proteins 1HNY , 2I3Y , and 5O40 , respectively. Among all isolated phthalates, HR5 can be a lead compound as it showed the best binding affinity with human pancreatic α-amylase (Δ G = −5.9 kcal/mol) and displayed a minimum inhibition concentration (IC 50 ) of 11.69 μM among all phthalates. Compound HR1 was the best docked and scored compound for inhibiting glutathione peroxidase; however, HR2 possessed the lowest binding score of −5.0 kcal/mol, thus indicating the highest potential among isolated phthalates for inhibiting the superoxide dismutase. Furthermore, the top-ranked docked ligand–protein complex for each protein was assessed for stability of protein and complex mobility by molecular dynamics simulation using the IMOD server.

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Section: Plant Collection Identification and Extractionmentioning

confidence: 97%

Plant-Based Bioactive Phthalates Derived from Hibiscus rosa-sinensis: As In Vitro and In Silico Enzyme Inhibition

Yasmin,

Nazli,

Shafiq

et al. 2023

ACS Omega

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“…This hydroquinine derivative demonstrated inhibitory activity against glycogen synthesis with an IC50 of 8.794 µM vitro investigations. The inhibition of glycogen phosphorylase would significantly improve the management of DM, particularly type II (N. Shafiq et al 2022). Based on these results, stephonocol may be considered a promising plant-derived chemical that offers type II diabetics a suitable means of managing their blood glucose levels.…”

Section: Caralluma Europaeamentioning

confidence: 99%

Natural Remedies for the Treatment of Diabetes Mellitus

Manzoor Ahmed,

Almas Zahid

2022

Phytopharmacological Com.

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Natural remedies have been used for decades to treat a variety of diseases. Diabetes mellitus (DM) is still one of the most common chronic diseases that cause death. Aside from its complex pathophysiology, it has been shown to be widespread in both developed and developing countries. The current pharmacological treatment has contributed to a significant reduction in disease occurrence. However, the economic burden and various pharmacological and non-pharmacological side effects associated with them, have necessitated the development of alternative naturally-derived treatment options. This review focuses on how safer, more effective, and less expensive treatments can help reduce diabetes-linked mortality rates. Furthermore, a wide range of medicinal plants with varying α-glucosidase and α-amylase inhibitory activity and improved IC50 and LD50 values evaluated in-vivo and in-vitro have been discussed. This review can help open new treatment paths for diabetes mellitus through new plant-derived medicines.

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Characterization of stenocephol from Seriphidium stenocephalum as potent HepG2 cell growth and glycogen phosphorylase inhibitor

Cited by 2 publications

References 38 publications

Plant-Based Bioactive Phthalates Derived from Hibiscus rosa-sinensis: As In Vitro and In Silico Enzyme Inhibition

Plant-Based Bioactive Phthalates Derived from Hibiscus rosa-sinensis: As In Vitro and In Silico Enzyme Inhibition

Natural Remedies for the Treatment of Diabetes Mellitus

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