Characterization of Staphylococcus Species by Ribosomal RNA Gene Restriction Patterns

Buyser, Marie-Laure De; Morvan, Anne; Grimont, Francine; Solh, Névine El

doi:10.1099/00221287-135-4-989

Cited by 101 publications

(98 citation statements)

References 20 publications

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“…This property is undoubtedly due to the fact that the method is applicable to the taxonomy of staphylococci [23,24]. It nonetheless discriminated between methicillin-susceptible and -resistant strains.…”

Section: Discussionmentioning

confidence: 99%

Clonal study of enterotoxin-B producing strains ofStaphylococcus aureus

Renaud¹,

Bornstein²,

Meugnier³

et al. 1994

Epidemiol. Infect.

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SUMMARYSixty-nine Staphylococcus aureas strains, 39 of which produced staphylococcal enterotoxin B (SEB + ) and 14 of which were associated with toxic shock (TS +), were studied using the following markers: serotyping, phage typing, antibiotyping, ribotyping, zymotyping and pulsed-field electrophoresis typing. Analysis of the results showed that the enterotoxin B producing strains were derived from at least three clones: the first two consisted of methicillin-susceptible strains, while the third included the methicillin-resistant (MRSA) strains. TS + strains of nongenital origin appeared to be distributed between the three clones, with no specific characters. INTRODUCTIONThe toxic shock syndrome toxin-i (TSST-1) of Staphylococcus aureus is responsible for staphylococcal toxic shock (TS). Most of the strains of genital origin and 50 % of others belong to the same clone [1]. Among the other S. aureus toxins, staphylococcal enterotoxin B (SEB) has been associated with disease recognized post-mortem [2,3]. Responsible mainly for food poisoning, it has also been associated with toxic shock [4][5][6]. The properties of SEB-producing strains (SEB+) are well documented; Asheshov and co-workers [7] demonstrated that the strains in the 94/96 phage complex were very often SEB+; Melconian and colleagues [8] observed that these strains were lysed mainly by phages of groups II and V; and Dornbusch and Hallander [9] have demonstrated a relationship between oxacillin resistance and toxin production. Recently, Lee and colleagues [10] have shown that TS-associated strains produced either TSST-1, enterotoxin B or, exceptionally, both; they have also shown that SEB+ strains belonged to the same zymotype, suggesting a clonal origin. The aim of the present study was to confirm or refute the existence of an enterotoxin-B producing cell clone by studying the phenotypic and genotypic characters of SEB + strains (whether or not associated with TS) as well as non-producing strains, using six different

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Section: Discussionmentioning

confidence: 99%

Clonal study of enterotoxin-B producing strains ofStaphylococcus aureus

Renaud¹,

Bornstein²,

Meugnier³

et al. 1994

Epidemiol. Infect.

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“…In other words, there is only a limited number of stable discriminating characteristics to distinguish one taxon supported phylogenetically from another by phenotypic tests [17]. Although our cluster groups correlated well with the species groups and other genealogical taxonomies such as ribotypes [6][7][8], several disagreements were found. Two established species, S. felis and S. simulans, which are indistinguishable by several species differentiation tables of phenotypic tests [17,20,[23][24][25] except for bacitracin resistance [21], were distinct in the phylogenetic tree with a relatively deep branch.…”

Section: Discussionmentioning

confidence: 61%

Phylogenetic Analyses of Staphylococcus Based on the 16S rDNA Sequence and Assignment of Clinical Isolates from Animals.

Takahashi

Kaneko

Mori

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. The nucleotide sequences of the 16S rDNA in 17 strains of 16 taxa of the genus Staphylococcus were determined. The sequences were compared phylogenetically together with the gene sequences of 10 (including 7 other species) Staphylococcus species retrieved from the DNA Data Bank of Japan. Although the primary and secondary structures of most of Staphylococcus species were very similar (homology values 96.4% or more) except for S. caseolyticus MAFF 911387 T (homology values 95.4% or less), the 23 staphylococcal species were divided into 10 groups based on similarity, evolutionary distance and phylogenetic tree analysis. Nucleotide stretches in several variable domains in the 16S rDNA sequence appeared to be specific for the bacterial groups or the species. By comparing such characteristics in the sequence and phylogenies of 5 staphylococcal clinical isolates from bovine mastitis, canine and feline pyoderma, and feline urogenital syndrome with the information obtained in this study, the species level of each organism was identified. -KEY WORDS: rDNA, 16S ribosomal RNA, Staphylococcus.J. Vet. Med. Sci. 59(9): 775-783, 1997 have indicated the existence of species-specific nucleotide stretches within the gene [3,28,39], however, the number of the species determined their sequence were limited. The aim of this study was to detect subtle differences between staphylococcal species by comparing their sequence patterns of the 16S rDNA and to understand the phylogenetic relationships. We also examined the usefulness of comparative gene analysis for precise identification of staphylococcal isolates in the veterinary field. MATERIALS AND METHODS

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“…It is now possible to rapidly identify pathogenic microorganisms such as Salmonella (Ashok et al, 1994 and Jorge et al, 1992) and Escherichia coli 0157 (Ito et al, 1990;Jackson et al, 1987;Takao et al, 1988 andWeinstein et al, 1988), and study the source of pathogenic microorganisms such as the dimethoxyphenyl penicillintolerant Staphylococcus aureus (De Buyser et al 1989;Kreiswirth et al, 1993;Musser and Kapur, 1992;Wada et al, 1991 and which cause nosocomial infections. In Japan, there have been several mass outbreaks of infectious disease caused by O 157 and the source of infection was made clear in part by PFGE (the pulse field gel electrophoresis) analysis (Wada et al, 1997) and RAPD analysis (lzumiya et al, 1997;Madico et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, using the RAPD method which has been assessed as superior to general DNA Fingerprinting techniques (Akopyanz et al, 1992;Brousseau et al, 1993;Makino et al, 1994;Torriani et al, 1999;Welsh and McClelland, 1990;Williams et al, 1990) and Southern blotting (De Buyser et al, 1989 andMangin et al, 1994;Marilena et al, 1992;Pot et al, 1993 andTatzel et al, 1994) targeting the 23S rRNA gene (Ludwig et al, 1992) (The Ribotyping method), we attempted to identify the source of contamination. For RAPD, we referred to lzumiya et al (1997) on screening with a primer for E. coli 0157: H7.…”

Section: Introductionmentioning

confidence: 99%