Characterization of Staphylococcus roterodami sp. nov., a new species within the Staphylococcus aureus complex isolated from a human foot infection

Schutte, Annemiek H J; Strepis, Nikolaos; Zandijk, Willemien H A; Bexkens, Michiel L.; Bode, L.; Klaassen, Corné H. W.

doi:10.1099/ijsem.0.004996

Cited by 22 publications

(17 citation statements)

References 20 publications

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“…A monophyletic group containing M. lentus , M. sciuri , M. vitulinus and M. stepanovicii was identified as the sister group to all other Staphylococcus analyzed, a result in line with the recent assignment of these four species to the new Mammalliicoccus genus [ 20 ]. As expected, norAI , norAII, and norAIII from S. aureus formed a strongly supported sub-cluster (bootstrap = 100%), together with S. argentus , S. schweitzeri , S. roterodami , and S. singaporensis —four recently renamed species that were previously described as divergent lineages of S. aureus [ 21 , 22 , 23 ]. Except for M. fleurettii , also recently reassigned to the new Mammalliicoccus genus, most relations within the norA gene phylogenetic tree are in general agreement with the evolutionary relations of staphylococci based on the phylogenetic tree of 16S-23S rRNA region obtained by Kosecka-Strojel et al (2019) [ 24 ], highlighting the presence of norA in the early branching of the Staphylococcus genus.…”

Section: Resultssupporting

confidence: 66%

Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus

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et al. 2022

IJMS

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NorA is one of the main native MDR efflux pumps of Staphylococcus aureus, contributing to reduced susceptibility towards fluoroquinolones and biocides, but little is known about its variability within S. aureus or its distribution and conservation among other staphylococci. We screened for sequences homologous to S. aureus norA and found it in 61 out of the 63 Staphylococcus species described. To the best of our knowledge, this is the first study to report the occurrence of norA across the Staphylococcus genus. The norA phylogenetic tree follows the evolutionary relations of staphylococci and the closely related Mammalliicoccus genus. Comparative analyses suggest a conservation of the NorA function in staphylococci. We also analyzed the variability of norA within S. aureus, for which there are several circulating norA alleles, differing up to 10% at the nucleotide level, which may hamper proper norA detection. We demonstrate the applicability of a PCR-based algorithm to detect and differentiate norA alleles in 52 S. aureus representing a wider collection of 89 isolates from different hosts. Our results highlight the prevalence of norAI and norAII in different settings and the association of norA alleles with specific S. aureus clonal lineages. Ultimately, it confirms the applicability of our PCR-based algorithm to rapidly detect and assign the different norA alleles, a trait that may impact antimicrobial efflux capacity and the search for potential NorA inhibitors.

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Section: Resultssupporting

confidence: 66%

Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus

Ferreira

Abrantes

Costa

et al. 2022

IJMS

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“…S. aureus, S. schweitzeri, S. argenteus, S. roterodami and S. singarporensis form the S. aureus complex [3][4][5][6]. The members of the S. aureus complex other than S. schweitzeri will be briefly introduced here.…”

Section: The S Aureus Complexmentioning

confidence: 99%

“…S. roterodami was first described in 2021 by Schutte et al [6]. This species was isolated from a human foot wound infection in the Netherlands.…”

Section: The S Aureus Complexmentioning

confidence: 99%

Staphylococcus schweitzeri—An Emerging One Health Pathogen?

et al. 2022

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The Staphylococcus aureus-related complex is formed by the Staphylococcus aureus, Staphylococcus schweitzeri, Staphylococcus argenteus, Staphylococcus roterodami and Staphylococcus singaporensis. Within this complex, S. schweitzeri is the only species mainly found in African wildlife, but it is rarely detected as a colonizer in humans or as a contaminant of fomites. The few detections in humans are most likely spillover events after contact with wildlife. However, since S. schweitzeri can be misidentified as S. aureus using culture-based routine techniques, it is likely that S. schweitzeri is under-reported in humans. The low number of isolates in humans, though, is consistent with the fact that the pathogen has typical animal adaptation characteristics (e.g., growth kinetics, lack of immune evasion cluster and antimicrobial resistance); however, evidence from selected in vitro assays (e.g., host cell invasion, cell activation, cytotoxicity) indicate that S. schweitzeri might be as virulent as S. aureus. In this case, contact with animals colonized with S. schweitzeri could constitute a risk for zoonotic infections. With respect to antimicrobial resistance, all described isolates were found to be susceptible to all antibiotics tested, and so far no data on the development of spontaneous resistance or the acquisition of resistance genes such the mecA/mecC cassette are available. In summary, general knowledge about this pathogen, specifically on the potential threat it may incur to human and animal health, is still very poor. In this review article, we compile the present state of scientific research, and identify the knowledge gaps that need to be filled in order to reliably assess S. schweitzeri as an organism with global One Health implications.

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“… Staphylococcus argenteus belongs to the Staphylococcus aureus complex (SAC) together with Staphylococcus schweitzeri , S. aureus , and the recently described Staphylococcus roterodami ( 1 ) and Staphylococcus singaporensis ( 2 ). S. argenteus was first described in 2006 ( 3 ) and was shown to belong to multilocus sequence typing (MLST) clonal complex 75 (CC75) of S. aureus .…”

Section: Introductionmentioning

confidence: 99%

Genetic Characteristics of Methicillin-Resistant Staphylococcus argenteus Isolates Collected in the Dutch National MRSA Surveillance from 2008 to 2021

et al. 2022

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We show that MRSArg has been circulating in the Netherlands since at least 2008. Although MRSArg is distinct from MRSA, it has a comparable population structure and carries similar resistance and virulence genes. The Dutch national MRSA surveillance has been expanded to include other methicillin-resistant members of the S. aureus complex, such as S. argenteus and Staphylococcus schweitzeri .

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Characterization of Staphylococcus roterodami sp. nov., a new species within the Staphylococcus aureus complex isolated from a human foot infection

Cited by 22 publications

References 20 publications

Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus

Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus

Staphylococcus schweitzeri—An Emerging One Health Pathogen?

Genetic Characteristics of Methicillin-Resistant Staphylococcus argenteus Isolates Collected in the Dutch National MRSA Surveillance from 2008 to 2021

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