Characterization of Staphylococcus aureus biofilms via crystal violet binding and biochemical composition assays of isolates from hospitals, raw meat, and biofilm-associated gene mutants

Ball, Ashley L.; Augenstein, Emilee D.; Wienclaw, Trevor M.; Richmond, Bradley C.; Freestone, Courtney A.; Lewis, Jessica M.; Thompson, Jared S.; Pickett, Brett E.; Berges, Bradford K.

doi:10.1016/j.micpath.2022.105554

Cited by 8 publications

(11 citation statements)

References 54 publications

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“…The crystal violet staining was used to determine the E. coli O157:H7 biofilm formation and the measures were according to Shu et al [31] and Ball et al [32] . After cultured at 37℃ for 12, 24, 36, 48 h, the supernatant NB was removed and the E. coli O157:H7 biofilm was washed with 0.85 % NaCl solution twice and then air-dried for 1 h. After air-dried, the E. coli O157:H7 biofilm was stained with 200 μL of 1 % (w/v) crystal violet for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7

Sun,

Shen,

Pan

et al. 2024

Ultrasonics Sonochemistry

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Section: Methodsmentioning

confidence: 99%

Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7

Sun,

Shen,

Pan

et al. 2024

Ultrasonics Sonochemistry

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“…The biofilm mass was quantified through the crystal violet staining method, as previously reported. 41,42 In brief, after the growth of biofilms, the suspension was poured into the microplates and the biofilms were washed twice with 0.1 M PBS to remove the suspended cells. Then, the biofilms were fixed with methanol solution for 15 min and dyed with 200 μL 0.1% crystal violet (CV) solution for 30 min.…”

Section: Cultivation and Quantification Of Biofilmsmentioning

confidence: 99%

Multiomics reveals the mechanism of B. longum in promoting the formation of mixed-species biofilms

Xu¹,

Xiao²,

Wang³

et al. 2023

Food Funct.

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“…S. aureus strain JE2 was grown on each surface for 36 hours. JE2 was chosen because it is a USA300 strain, which is clinically relevant, and because it was found to have a bio lm matrix structure that was representative of many S. aureus isolates in quantities of polysaccharide, protein, and extracellular DNA [22]. After 36 hours, the bio lm was washed to remove unattached cells.…”

Section: Optimizing Cicnt Diameter To Reduce Bio Lm Growthmentioning

confidence: 99%

“…To determine if the reduction in adherent bacteria was consistent among different strains of S. aureus, we tested six additional isolates of S. aureus (JE2, used in previous experiments, is included as a reference). The clinical isolates chosen were found in a previous publication to represent a variety of relative bio lm strengths, with SH1000 forming the strongest bio lm and HA3 the weakest [22]. Additionally, both methicillin resistant and susceptible isolates were tested.…”

Section: Bio Lm Growth Is Slower On Cicnt Surfaces Than On Ti Surfacesmentioning

confidence: 99%

“…The six other strains were chosen since they represent a variety of bio lm compositions. These strains have previously been tested for their relative composition of bio lm protein, polysaccharide, and extracellular DNA [22]. SH1000 (BEI Resources NR-55396) is a methicillin-sensitive human isolate with the addition of the rbsU gene for ribose uptake.…”

Section: Bacterial Strainsmentioning

confidence: 99%

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Carbon-Infiltrated Carbon Nanotubes Inhibit the Development of Staphylococcus aureus Biofilms

Bowden,

Wells,

Miller

et al. 2023

Preprint

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Background: Staphylococcus aureus forms biofilms that cause considerable morbidity and mortality in patients who receive implanted devices such as prosthetics or fixator pins. An ideal surface for such medical devices would inhibit biofilm growth. Recently, it was reported that surface modification of stainless steel materials with carbon-infiltrated carbon nanotubes (CICNT) inhibits the growth of S. aureus biofilms. The purpose of this study was to investigate this antimicrobial effect on titanium materials with CICNT coated surfaces in a variety of surface morphologies and across a broader spectrum of S. aureus isolates. Results: Study samples of CICNT-coated titanium, and control samples of bare titanium, a common implant material, were exposed to S. aureus. Viable bacteria were removed from adhered biofilms and quantified as colony forming units. Scanning electron microscopy was used to qualitatively analyze biofilms both before and after removal of cells. The CICNT surface was found to have significantly fewer adherent bacteria than bare titanium control surfaces, both via colony forming unit and microscopy analyses. This effect was most pronounced on CICNT surfaces with an average nanotube diameter of 150 nm, showing a 2.5-fold reduction in adherent bacteria. Since S. aureus forms different biofilm structures by isolate and by growth conditions, we tested 7 total isolates and found a significant reduction in the biofilm load in six out of seven S. aureus isolates tested. To examine whether the anti-biofilm effect was due to the structure of the nanotubes, we generated an unstructured carbon surface. Significantly more bacteria adhered to a nonstructured carbon surface than to the CICNT surface, suggesting that the topography of the nanotube structure itself has anti-biofilm properties. Conclusions: The CICNT surface possesses antimicrobial properties that result in fewer adherent S. aureus bacteria. These antimicrobial properties are consistent across multiple isolates of S. aureus and are affected by nanotube diameter. The experiments performed in this study suggest that this effect is due to the nanostructure of the CICNT surface.

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Characterization of Staphylococcus aureus biofilms via crystal violet binding and biochemical composition assays of isolates from hospitals, raw meat, and biofilm-associated gene mutants

Cited by 8 publications

References 54 publications

Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7

Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7

Multiomics reveals the mechanism of B. longum in promoting the formation of mixed-species biofilms

Carbon-Infiltrated Carbon Nanotubes Inhibit the Development of Staphylococcus aureus Biofilms

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