Characterization of stable lysozyme-entrapped polyion complex (PIC) micelles with crosslinked core by glutaraldehyde

Complex coacervate core micelles

“…Besides co-micellization with DNA and RNA, other biopolymers, such as proteins, peptides, and enzymes, may also be encapsulated to preserve and/or promote stability and function, as was demonstrated for KALA, [88,140] lysozyme, [121,122,[141][142][143][144][145][146]150] trypsin, [148,149] myoglobin, [104] and BSA [62]. Circular dichroism (CD) experiments on lysozyme-containing C3Ms demonstrated that the secondary structure of free and C3M incorporated lysozyme is identical [144].…”

“…Circular dichroism (CD) experiments on lysozyme-containing C3Ms demonstrated that the secondary structure of free and C3M incorporated lysozyme is identical [144]. As is the case for DNA-containing C3Ms, complexation with oppositely charged copolymers, typically improves protein/enzyme stability with respect to ionic strength, dilution, denaturation by urea, [149] protease attack, [104] which may be further enhanced by core crosslinking [122,148,149] or end-functionalization of the core-block of the copolymer with hydrophobic groups, such as pyrenyl [121]. Trypsin and lysozyme activity toward small substrates (i.e., substrates that can diffuse into the micellar core) is promoted when embedded in C3Ms; i.e.…”

Hierarchical Self‐Assembly in Solutions Containing Metal Ions, Ligand, and Diblock Copolymer

“…In this way, C3Ms have great potential as nanoscale carriers for the delivery of charged compounds such as DNA and proteins. [4][5][6] Herein we address the question as to whether it is possible to fabricate self-assembled objects such as C3Ms with reversible supramolecular polymers, which are self-assembled chains themselves. Such chains are typically responsive: their chain length is not fixed but can adjust to variables such as temperature, concentration, etc.…”

pH‐Switchable Complexation between Double Hydrophilic Heteroarm Star Copolymers and a Cationic Block Polyelectrolyte

“…[34] Taking this perspective, the selective alteration of the solubility of one block, realized via polyelectrolyte complexation, [35][36][37][38][39][40][41] hydrogen-bonded complexation, [42][43][44][45] stereocomplexation, [46] and metal-ion complexation [47][48][49][50] have been successfully employed to fabricate colloidal nanoparticles, possessing micelle-like core-shell structures.…”