Characterization of specific egg yolk immunoglobulin (IgY) against mastitis<i>-</i>causing<i>Staphylococcus aureus</i>

Zhen, Yuhong; Jin, Lei; Guo, Jie; Li, X.-Y.; Li, Z.; Fang, Rui; Xu, You-Hai

doi:10.1111/j.1365-2672.2008.03920.x

Cited by 34 publications

(26 citation statements)

References 33 publications

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“…IgY can thus be an alternative method for providing infection control. Comparing our results with those obtained by Zhen shows that the IgY produced here was effective at lower concentrations (Zhen et al 2008).…”

Section: Discussionsupporting

confidence: 85%

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Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

Guimarães¹,

Amaral²,

Rangel³

et al. 2009

Arch. Immunol. Ther. Exp.

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Introduction: The aim was to evaluate the ability of egg yolk antibody (IgY) in blocking Staphylococcus aureus growth in vitro. Materials and Methods: Specific IgY was produced by immunizing hens with formalin-killed S. aureus (ATCC 33593). Specific IgY against S. aureus was obtained from the yolks of their eggs with a carrageenan solution. IgY was identified by SDS-PAGE and Western blot and its activity against S. aureus was tested by ELISA. A growth inhibition assay and protein concentration determination were also conducted. Results: ELISA indicated that the IgY was specific to the antigen; this activity was confirmed by Western blotting. The growth of S. aureus was inhibited by the specific IgY at concentrations of 1-5 µg/ml The bacteriostatic function of IgY appeared to result possibly from the interaction of IgY with surface components of S. aureus. In vitro experiments showed that the immunoglobulin from egg yolk interfered with the culture growth of the S. aureus. Conclusion: These findings indicate that eggs from hens immunized with appropriate antigens are a potentially useful source of passive immunity.

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Section: Discussionsupporting

confidence: 85%

“…This assay was conducted to investigate whether the anti-S. aureus IgY could inhibit S. aureus growth in liquid medium as described by the method of Zhen with some modifications (Zhen et al 2008). The extracted IgY (titer 6400) was dissolved in TSB at concentrations of 1, 2.5, and 5 µg/ml.…”

Section: Growth Inhibition Assaymentioning

confidence: 99%

Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

Guimarães¹,

Amaral²,

Rangel³

et al. 2009

Arch. Immunol. Ther. Exp.

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“…IgY antibodies developed in chickens, using antigens from S. aureus were tested by several authors. Toxigenic S. aureus strains had their growth abrogated in vitro by native antigens of S. aureus (9, 24, 32). Others described antibodies from serum against native SEC (23) and recombinant SEC (26), but this is the first work to describe development of IgY in ostriches immunized with SEC recombinant protein, and tested against growth of S. aureus strains from different origin.…”

Section: Resultsmentioning

confidence: 99%

Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk

Tobias

Garcia

Kanashiro

et al. 2012

Braz. J. Microbiol.

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Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of sub products of ostrich raising, non fertilized eggs of four selected birds were utilized for development of polyclonal IgY antibodies. The birds were immunized (200ug/animal) with purified recombinant staphylococcal enterotoxin C (recSEC) and synthetic recRAP, both derived from S. aureus, and recBFPA and recEspB involved in E. coli pathogenicity, diluted in FCA injected in the braquial muscle. Two subsequent immunization steps with 21 days intervals were repeated in 0,85% saline in FIA. Blood and eggs samples were collected before and after immunization steps. Egg yolk immunoglobulins were purified by precipitation with 19% sodium sulfate and 20% ammonium sulphate methodologies. Purified IgY 50μL aliquots were incubated in 850μL BHI broth containing 50μL inoculums of five strains of S. aureus and five strains of E.coli during four hours at 37°C. Growth inhibition was evaluated followed by photometry reading (DO550nm). Egg yolk IgY preparation from hiperimmunized birds contained antibodies that inhibited significantly (p<0,05) growth of strains tested. Potential use of ostrich IgY polyclonal antibodies as a diagnostic and therapeutic tool is proposed for diseased animals.

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“…An in vitro study demonstrated that IgY has bacterial opsonizing properties that improve bacterial phagocytosis. 26 Thus, the clinical impact of IgY prophylaxis is perhaps attributable to immunomodulatory mechanisms that enhance phagocytic activity and subsequent bacterial clearance in the airways. To further explore the plausible opsonizing capacity of anti-Pseudomonas aeruginosa IgY antibodies, in vitro assays evaluating the activity of phagocytes exposed to various strains of P. aeruginosa were established.…”

Section: Introductionmentioning

confidence: 99%

Anti-Pseudomonas aeruginosaIgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils

Thomsen

Christophersen

Jensen

et al. 2016

Human Vaccines & Immunotherapeutics

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Moderation of polymorphonuclear neutrophils (PMNs) as part of a critical defense against invading pathogens may offer a promising therapeutic approach to supplement the antibiotic eradication of Pseudomonas aeruginosa infection in non-chronically infected cystic fibrosis (CF) patients. We have observed that egg yolk antibodies (IgY) harvested from White leghorn chickens that target P. aeruginosa opsonize the pathogen and enhance the PMN-mediated respiratory burst and subsequent bacterial killing in vitro. The effects on PMN phagocytic activity were observed in different Pseudomonas aeruginosa strains, including clinical isolates from non-chronically infected CF patients. Thus, oral prophylaxis with anti-Pseudomonas aeruginosa IgY may boost the innate immunity against Pseudomonas aeruginosa in the CF setting by facilitating a rapid and prompt bacterial clearance by PMNs.

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Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causingStaphylococcus aureus

Cited by 34 publications

References 33 publications

Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk

Anti-Pseudomonas aeruginosaIgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils

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