Characterization of species associated with Pythium soft rot of ginger and evaluation of Pythium Oligandrum as a biocontrol

Le, Duy Phu

doi:10.14264/uql.2016.618

Cited by 5 publications

(3 citation statements)

References 191 publications

(469 reference statements)

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“…A similar experiment using a different P. oligandrum isolate to control PSR soft-rot of ginger caused by a P. myriotylum isolated from infected ginger in Australia did not reduce the disease symptoms on ginger plants ( Le, 2016 ). It was speculated by Le (2016) that the lack of a control effect may have been in part due to poor colonization of the soil by the P. oligandrum isolate (vermiculite was used to grow the ginger plants inoculated with GAQ1) or the ineffective application of the oospore suspension by soil drenching (mycelia of the GAQ1 isolate was added directly into the vermiculite). However, there is support for P. oligandrum to control other diseases caused by P. myriotylum where previously it was shown that P. oligandrum was able to reduce soybean damping-off caused by P. myriotylum ( You et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…To our knowledge, there are no reports in the literature of antagonism of P. oligandrum toward P. myriotylum or to the control of diseases caused by P. myriotylum . An unpublished report showed that an isolate of P. oligandrum was antagonistic toward P. myriotylum in plate confrontation assays, but there was no control effect on Pythium soft-rot (PSR) of ginger caused by Pythium myriotylum ( Le, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

et al. 2021

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Biological control is a promising approach to suppress diseases caused by Pythium spp. such as Pythium soft rot of ginger caused by P. myriotylum. Unusually for a single genus, it also includes species that can antagonize Pythium plant pathogens, such as Pythium oligandrum. We investigated if a new isolate of P. oligandrum could antagonize P. myriotylum, what changes occurred in gene expression when P. oligandrum (antagonist) and P. myriotylum (host) interacted, and whether P. oligandrum could control soft-rot of ginger caused by P. myriotylum. An isolate of P. oligandrum, GAQ1, recovered from soil could antagonize P. myriotylum in a plate-based confrontation assay whereby P. myriotylum became non-viable. The loss of viability of P. myriotylum coupled with how P. oligandrum hyphae could coil around and penetrate the hyphae of P. myriotylum, indicated a predatory interaction. We investigated the transcriptional responses of P. myriotylum and P. oligandrum using dual-RNAseq at a stage in the confrontation where similar levels of total transcripts were measured from each species. As part of the transcriptional response of P. myriotylum to the presence of P. oligandrum, genes including a subset of putative Kazal-type protease inhibitors were strongly upregulated along with cellulases, elicitin-like proteins and genes involved in the repair of DNA double-strand breaks. In P. oligandrum, proteases, cellulases, and peroxidases featured prominently in the upregulated genes. The upregulation along with constitutive expression of P. oligandrum proteases appeared to be responded to by the upregulation of putative protease inhibitors from P. myriotylum, suggesting a P. myriotylum defensive strategy. Notwithstanding this P. myriotylum defensive strategy, P. oligandrum had a strong disease control effect on soft-rot of ginger caused by P. myriotylum. The newly isolated strain of P. oligandrum is a promising biocontrol agent for suppressing the soft-rot of ginger. The dual-RNAseq approach highlights responses of P. myriotylum that suggests features of a defensive strategy, and are perhaps another factor that may contribute to the variable success and durability of biological attempts to control diseases caused by Pythium spp.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

et al. 2021

View full text Add to dashboard Cite

show abstract

“…A similar experiment using a different P. oligandrum isolate to control PSR soft-rot of ginger caused by a P. myriotylum isolated from infected ginger in Australia did not reduce the disease symptoms on ginger plants (Le, 2016). It was speculated by Le (2016) that the lack of a control effect may have been in part due to poor colonization of the soil by the P. oligandrum isolate (vermiculite was used to grow the ginger plants inoculated with GAQ1) or the ineffective application of the oospore suspension by soil drenching (mycelia of the GAQ1 isolate was added directly into the vermiculite). However, there is support for P. oligandrum to control other diseases caused by P. myriotylum where previously it was shown that P. oligandrum was able to reduce soybean damping-off caused by P. myriotylum (You et al, 2019).…”

Section: P Oligandrum Gaq1 Has Control Effect On Pythium Soft-rot Of P Myriotylum Infected Gingermentioning

Characterization of species associated with Pythium soft rot of ginger and evaluation of Pythium Oligandrum as a biocontrol

Cited by 5 publications

References 191 publications

Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

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