Characterization of soybean tissue culture cell lines resistant to methionine analogs

Madison, James T.; Thompson, John F.

doi:10.1007/bf00272734

Cited by 11 publications

(11 citation statements)

References 16 publications

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“…Although the resistant phenotype with high essential amino acid concentration could be isolated by somaclonal variation (Kim et al, 2004), mutagen treatment seem to be indispensable to the selection of maize callus cultures resistant to 5-methyl-DL DLtryptophan (5-MT), S-2-aminoethyl-L L -cysteine (AEC) and L L -lysine plus L L -threonine (LT) (Miao et al, 1988) and of Nicotiana sylvestris protoplast cultures resistant to AEC (Negrutiu et al, 1984). Furthermore, high NTG concentration resulted in an increase in ethionine resistant frequency in A. adsurgens (Table 1), similar results have been reported for soybean (Madison and Thompson, 1988) and Vigna Sinensis cell cultures (Ahmed et al, 1986).…”

Section: Isolation Of Resistant Colony Linessupporting

confidence: 84%

“…ETH is a chemical analog of methionine and has been used to select variants with increased concentrations of free methionine in several species including alfalfa (Reisch et al, 1981), carrot (Widholm, 1976), potato (Langille et al, 1998), soybean (Madison and Thompson, 1988;Imsande, 2001), tobacco (Gonzales et al, 1984), Arabidopsis thaliana (Inaba et al, 1994;Goto et al, 2002;Shen et al, 2002), Aspargua officinalis (Curtiss et al, 1987) and Vigna sinensis (Ahmed et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Selection of ethionine-resistant variants with increased accumulation of methionine from embryogenic protoplasts of the forage legume Astragalus adsurgens

Luo

Zha

Shi

2005

Plant Cell Tiss Organ Cult

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In vitro selection was carried out to obtain ethionine-resistant plants with increased contents of free methionine in the vegetative tissues of the forage legume Astragalus adsurgens Pall. Three-week-old cell colonies were derived from protoplasts mutagenized with N-methyl-N-nitrosoguanidine from embryogenic callus and were selected with 0.6 mM ethionine. Four colony lines were isolated and their resistance to ethionine was 7-8 times that of the wild-type callus. No plant regeneration occurred on these colony lines in the differentiation medium containing ethionine. Only one colony line (R-1) regenerated plants through somatic embryogenesis in the absence of ethionine. Stem and leaf segments from the regenerated plants showed the same potential to produce callus in the presence of ethionine as in the absence of ethionine. The formed callus kept continuously growing in ethionine-containing medium. Free amino acid analysis revealed that colony line R-1, its regenerated plants and callus from the regenerated plants accumulated methionine at levels at 5-9 times higher than in wild-type. These results suggested that ethionine resistance and methionine over-accumulation were also expressed at plant level. Thus, the obtained resistant colony line that could regenerate plants with over-accumulation of methionine might provide an alternative approach to improve the nutritional quality of this forage.

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Section: Isolation Of Resistant Colony Linessupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Selection of ethionine-resistant variants with increased accumulation of methionine from embryogenic protoplasts of the forage legume Astragalus adsurgens

Luo

Zha

Shi

2005

Plant Cell Tiss Organ Cult

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“…Ethionine is a toxic analogue of Met (Alix 1982) that has been used to isolate Met over-accumulating mutants of bacteria (Kappy and Metzenberg 1965) and yeast (Sorsoli et al 1964, Mertz andSpence 1972). The phenotype of ethionine-resistance has also been used to successfully identify plant cell lines (Widholm 1976, Reish et al 1981, Gonzales et al 1984, Madison and Thompson 1988 and plant mutants , Bartlem et al 2000) that overaccumulate soluble Met.…”

Section: Introductionmentioning

confidence: 99%

A single-nucleotide mutation in a gene encoding S-adenosylmethionine synthetase is associated with methionine over-accumulation phenotype in Arabidopsis thaliana.

Goto

Ogi²,

Kijima³

et al. 2002

Genes Genet. Syst.

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Met-overaccumulating mutants provide a powerful genetic tool for examining both the regulation of the Met biosynthetic pathway and in vivo developmental responses of gene expression to altered Met levels. We have previously reported the identification of two Arabidopsis thaliana Met over-accumulation ( mto ) mutants, mto1-1 and mto2-1 , that carry mutations in the genes encoding cystathionine γ -synthase (CGS) and threonine synthase (TS), respectively. A third mutant, mto3-1 , has recently been reported to carry a mutation in the gene encoding S -adenosylmethionine synthetase 3 (SAMS3). Here, we report the isolation of a new ethionine-resistant A. thaliana mutant that over-accumulates soluble Met approximately 20-fold in young rosettes. The causal mutation was determined to be a single, recessive mutation that was mapped to chromosome 3. Sequence analysis identified a single nucleotide change in the gene encoding SAMS3 that was distinct from the mto3-1 mutation and altered the amino acid sequence of the enzyme active site. This mutation was therefore referred to as mto3-2 . Although Met over-accumulation in the mto3-2 mutant was similar to that in the mto2-1 mutant, CGS mRNA levels did not respond to the mto3-2 mutation and were similar to that in equivalent wild-type plants.

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“…Because of their similarity in chemical structure and composition, an increased concentration of methionine lessens the toxicity of low concentrations of ethionine. Thus, low concentrations of ethionine have been used successfully to select and identify methionine over‐producing variants, including soybean tissue culture lines (Gonzales et al, 1984; Inaba et al, 1994; Langille et al, 1998; Madison and Thompson, 1988). All 26 of the ethionine‐resistant soybean tissue culture lines isolated contained an elevated internal methionine concentration, the mean of which, 41.7 nmol g −1 fresh weight of callus, was 8.7 fold greater than that of the parental line (Madison and Thompson, 1988).…”

mentioning

confidence: 99%

Selection of Soybean Mutants with Increased Concentrations of Seed Methionine and Cysteine

Imsande

2001

Crop Science

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Soybean [Glycine max (L.) Merr.] protein, which is deficient in the sulfur amino acids, especially methionine, is consumed world‐wide by both humans and other animals. Methionine deficiency is caused by an abundance of the β‐chain of β‐conglycinin, a seed storage protein that lacks methionine. De novo synthesis of the β‐chain is inhibited by an elevated concentration of methionine. The objectives of our investigations were to mutagenize soybean seeds, characterize a methionine over‐producing phenotype, and select several methionine over‐producing genetic lines. Mutant lines with a methionine over‐producing phenotype were isolated and crossed. Seeds from a cross, designated H82 × 20a2, contained a normal seed nitrogen concentration and an 18% increase in seed sulfur concentration. The S/N atomic ratio of line H82 × 20a2 was 16.2% greater than that of the parental line. Amino acid analyses of seeds from the derived and parental lines revealed mole percentages of 1.84 and 1.51, respectively, for methionine and 1.685 and 1.32, respectively, for cysteine. Thus, seed methionine and cysteine concentrations of H82 × 20a2 were each approximately 20% greater than those of the parental line. Protein produced by the derived line may fulfill nutritional requirements for methionine and cysteine.

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Characterization of soybean tissue culture cell lines resistant to methionine analogs

Cited by 11 publications

References 16 publications

Selection of ethionine-resistant variants with increased accumulation of methionine from embryogenic protoplasts of the forage legume Astragalus adsurgens

Selection of ethionine-resistant variants with increased accumulation of methionine from embryogenic protoplasts of the forage legume Astragalus adsurgens

A single-nucleotide mutation in a gene encoding S-adenosylmethionine synthetase is associated with methionine over-accumulation phenotype in Arabidopsis thaliana.

Selection of Soybean Mutants with Increased Concentrations of Seed Methionine and Cysteine

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