Characterization of Sox9 in European Atlantic Sturgeon (Acipenser sturio)

Hett, Anne Kathrin; Pitra, Christian; Jenneckens, Ingo; Ludwig, Aaron T.

doi:10.1093/jhered/esi008

Cited by 30 publications

(17 citation statements)

References 44 publications

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“…Yet, Sox9 is dispensable for testis differentiation after sex determination (Barrionuevo et al, 2009), leaving its involvement in fish testis differentiation unclear. In a previous work on sturgeons, no evidence was observed for sox9 duplication (Hett et al, 2005), as is the case in teleost fish (Cresko et al, 2003; Zhou et al, 2003), and no sex‐specific differences were observed. The phylogenetic analyses of Sox9 evolution revealed a basal position for sturgeon sox9 ( Acipenser sturio , Hett et al, 2005, A. baerii present work).…”

Section: Discussionmentioning

confidence: 87%

“…In a previous work on sturgeons, no evidence was observed for sox9 duplication (Hett et al, 2005), as is the case in teleost fish (Cresko et al, 2003; Zhou et al, 2003), and no sex‐specific differences were observed. The phylogenetic analyses of Sox9 evolution revealed a basal position for sturgeon sox9 ( Acipenser sturio , Hett et al, 2005, A. baerii present work). In medaka and pejerrey ( Odontestes bonariensis ), sox9 has been proposed as being involved in the formation of testis structures, but not in testis differentiation (Nakamoto et al, 2005; Blasco et al, 2010).…”

Section: Discussionmentioning

confidence: 87%

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Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii

Berbejillo

Martinez-Bengochea

Bedó

et al. 2012

Molecular Reproduction Devel

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The molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. The sex differentiation period was investigated in the Siberian sturgeon, Acipenser baerii, by expression profiling of Sertoli cell transcription factors (dmrt1, sox9) that control testis differentiation in vertebrates; Leydig cell factors (cyp17a1, star) affecting androgen production; the androgen receptor (ar); a growth factor controlling testis development (igf1); and a gene coding for a gonadotropin hormone (lh). Two genes were characterised for the first time in the Siberian sturgeon (dmrt1, cyp17a1), while the others came from public databases. Sturgeon gonad development is very slow, with a late sexual differentiation time during their juvenile stage, and are still immature at 3 years of age. Immature fish showed a sex-dimorphic pattern; all the genes studied displayed a higher expression level in male gonads. We took advantage of the presence of juvenile fish with pre- and post-differentiated gonads (16 and 18 months old) to characterise them at the molecular level. The post-differentiated fish displayed a sex dimorphism of gene expression in their gonads for all genes studied, with the exception of sox9. The trends in undifferentiated fish lead us to propose that sturgeons undergoing male differentiation express high levels of Sertoli cell factors (dmrt1, sox9) and of genes involved in the production and receptivity of androgens (cyp17a1, star and ar) together with lh. Expression profiles and phylogenetic studies suggest that these genes are potential regulators of testis development in the Siberian sturgeon.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 87%

Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii

Berbejillo

Martinez-Bengochea

Bedó

et al. 2012

Molecular Reproduction Devel

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“…The sox9 gene is present in two copies (known as sox9a , sox9b or sox9a1 , sox9a2 ) in most of the fish species studied i.e. medaka, rice field eel, stickleback, zebrafish, fugu and rainbow trout [111-118] as a result of the teleost-specific genome duplication, except in sturgeon ( Acipenseridae ) [119,120]. The expression pattern of sox9 co-orthologs is more complicated in teleost fish.…”

Section: Reviewmentioning

confidence: 99%

Molecular players involved in temperature-dependent sex determination and sex differentiation in Teleost fish

2014

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The molecular mechanisms that underlie sex determination and differentiation are conserved and diversified. In fish species, temperature-dependent sex determination and differentiation seem to be ubiquitous and molecular players involved in these mechanisms may be conserved. Although how the ambient temperature transduces signals to the undifferentiated gonads remains to be elucidated, the genes downstream in the sex differentiation pathway are shared between sex-determining mechanisms. In this paper, we review recent advances on the molecular players that participate in the sex determination and differentiation in fish species, by putting emphasis on temperature-dependent sex determination and differentiation, which include temperature-dependent sex determination and genetic sex determination plus temperature effects. Application of temperature-dependent sex differentiation in farmed fish and the consequences of temperature-induced sex reversal are discussed.

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“…Research suggests that the sex-determination system in sturgeons is ZW for female and ZZ for male (58). Sox9, dmrt1, TrA-1, and Foxl2 have been identified as biomarkers for sex determination in some sturgeons (2,3,24,26), but no biomarker has been identified in Russian sturgeons. A previous study found that the stFSHb subunit was expressed at a higher level in 3 and 4 yr old fish than in 1 yr old fish and that the expression level of stFSHb in females was significantly higher than in males (28).…”

mentioning

confidence: 99%

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Chen

Xia²,

Shao

et al. 2016

Physiological Genomics

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Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads. Physiol Genomics 48: 464 -476, 2016. First published May 6, 2016 doi:10.1152/physiolgenomics.00113.2015.-As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated Ͼ60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.

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Characterization of Sox9 in European Atlantic Sturgeon (Acipenser sturio)

Cited by 30 publications

References 44 publications

Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii

Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii

Molecular players involved in temperature-dependent sex determination and sex differentiation in Teleost fish

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

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