Characterization of Some Microorganisms from Human Stool Samples and Determination of Their Effects on CT26 Colorectal Carcinoma Cell Line

Kahraman, Münevver; Karahan, Aynur Gül; Terzioğlu, Mustafa

doi:10.1007/s00284-022-02915-4

Cited by 4 publications

(4 citation statements)

References 47 publications

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“…hypotensive characteristics, as documented by researchers (Araya et al, 2002;Kahraman et al, 2022). In contrast to undergoing gastrointestinal tract degradation processes, EPS reaches the cecum and colon without being catabolized.…”

Section: F I G U R Ementioning

confidence: 77%

“…Scientific inquiry has revealed that EPS synthesized by probiotic microorganisms exhibits various physiological abilities. These encompass a range of beneficial properties such as antioxidative, immune‐modulatory, antitumor, anti‐inflammatory, antiviral, and hypotensive characteristics, as documented by researchers (Araya et al., 2002 ; Kahraman et al., 2022 ). In contrast to undergoing gastrointestinal tract degradation processes, EPS reaches the cecum and colon without being catabolized.…”

Section: Resultsmentioning

confidence: 98%

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Physicochemical properties and survival assessment of potential probiotics in a novel dairy drink during storage

Gedik,

Karahan

2023

Food Science & Nutrition

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A novel fermented dairy drink utilizing microbial strains displaying potential probiotic attributes was formulated. The study constituted several experimental cohorts, including Lactiplantibacillus plantarum AB6‐25, isolated from a human stool sample; Lacticaseibacillus casei K2, sourced from a koumiss sample; Lacticaseibacillus rhamnosus 3B7, derived from a traditional yogurt sample; and identical lactic acid bacteria (LAB) in combination with yeast (Saccharomyces boulardii T8‐3C from chicken feces) strains. Two distinct iterations of probiotic‐fermented dairy drinks were generated by introducing subcultured microorganism cultures: LAB strains at a concentration of 1% each (designated as combination A) and a blend of LAB strains at 1% each along with T8‐3C at 0.5% (designated as combination B) into both whole and semi‐skimmed milk matrices. The fermentation process persisted until the pH reached 4.6 under constant conditions of 37 ± 1°C. Subsequently, the samples were held at 4 ± 1°C for 15 days. The groups' physicochemical, microbiological, and sensory characteristics were determined on days 1, 8, and 15 of storage, and the protein profile was determined. Standardized regression analysis and principal component analysis evaluated the results. Fat content affected the changes in dry matter. pH decreased in all samples during storage, particularly in the yeast group. The microorganism group positively affected syneresis, whereas fat content and the interaction of fat content and the microorganism group had a negative effect. The most critical factor in the decrease in syneresis was the increase in fat content. LAB and yeasts maintained their probiotic effects during storage, with a viability level of approximately 109 and 108 cfu/mL, respectively.

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Section: F I G U R Ementioning

confidence: 77%

Section: Resultsmentioning

confidence: 98%

Physicochemical properties and survival assessment of potential probiotics in a novel dairy drink during storage

Gedik,

Karahan

2023

Food Science & Nutrition

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“…Our studies including that of others ( Chuah et al, 2019 , Happel et al, 2020 , Pramanick and Aranha, 2020 Dec 9 ) have demonstrated different strains of the same species also vary in their functional properties, hence investigation of several species need to be undertaken to identify the promising probiotic for particular function. Studies have shown that probiotics isolated from different environments or from the same environmental niche have varying functional properties (( Bazireh et al, 2020 , Kahraman et al, 2022 ). Our study further showed HeLa cells were more sensitive than SiHa cells; likewise, sensitivity of HeLa to chemotherapeutic agents has been observed by Xu et al (2012) .…”

Section: Discussionmentioning

confidence: 99%

Lactobacilli metabolites restore E-cadherin and suppress MMP9 in cervical cancer cells

Pawar

Aranha

2022

Current Research in Toxicology

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“…The attached cells were trypsinized, counted, and put into a 96-well plate, with 1 × 10 4 cells in each well. The plate was then incubated for 24 h at 37 °C and 5% CO2 to allow the cells to adhere to the bottom of the wells [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Antiproliferative effects of mesenchymal stem cells carrying Newcastle disease virus and Lactobacillus Casei extract on CT26 Cell line: synergistic effects in cancer therapy

Ghorbani Alvanegh,

Mirzaei Nodooshan,

Dorostkar

et al. 2023

Infect Agents Cancer

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Background and aims Colorectal Cancer (CRC) is a frequent malignancy with a high mortality rate. Specific inherited and environmental influences can affect CRC. Oncolytic viruses and bacteria in treating CRC are one of the innovative therapeutic options. This study aims to determine whether mesenchymal stem cells (MSCs) infected with the Newcastle Disease Virus (NDV) in combination with Lactobacillus casei extract (L. casei) have a synergistic effects on CRC cell line growth. Materials and methods MSCs taken from the bone marrow of BALB/c mice and were infected with the 20 MOI of NDV. Then, using the CT26 cell line in various groups as a single and combined treatment, the anticancer potential of MSCs containing the NDV and L. casei extract was examined. The evaluations considered the CT26 survival and the rate at which LDH, ROS, and levels of caspases eight and nine were produced following various treatments. Results NDV, MSCs-NDV, and L. casei in alone or combined treatment significantly increased apoptosis percent, LDH, and ROS production compared with the control group (P˂0.05). Also, NDV, in free or capsulated in MSCs, had anticancer effects, but in capsulated form, it had a delay compared with free NDV. The findings proved that L. casei primarily stimulates the extrinsic pathway, while NDV therapy promotes apoptosis through the activation of both intrinsic and extrinsic apoptosis pathways. Conclusions The results suggest that MSCs carrying oncolytic NDV in combination with L. casei extract as a potentially effective strategy for cancer immunotherapy by promoting the generation of LDH, ROS, and apoptosis in the microenvironment of the CT26 cell line.

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Characterization of Some Microorganisms from Human Stool Samples and Determination of Their Effects on CT26 Colorectal Carcinoma Cell Line

Cited by 4 publications

References 47 publications

Physicochemical properties and survival assessment of potential probiotics in a novel dairy drink during storage

Physicochemical properties and survival assessment of potential probiotics in a novel dairy drink during storage

Lactobacilli metabolites restore E-cadherin and suppress MMP9 in cervical cancer cells

Antiproliferative effects of mesenchymal stem cells carrying Newcastle disease virus and Lactobacillus Casei extract on CT26 Cell line: synergistic effects in cancer therapy

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