Characterization of soluble and bound EPS obtained from 2 submerged membrane bioreactors by 3D-EEM and HPSEC

Chabaliná, Liuba Domínguez; Pastor, Manuel Rodríguez; Prats, Daniel

doi:10.1016/j.talanta.2013.05.062

Cited by 50 publications

(6 citation statements)

References 27 publications

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“…However, membrane fouling is still the major obstacle preventing the universal application of MBR. Regarding the fouling mechanisms, organic foulants generated from biological process have been the main focus for previous studies, and extracellular polymeric substances (EPS) have been considered the major cause of membrane fouling in MBR [6][7][8][9]. Extracellular polymeric substances are a complex high molecular-weight mixture of polymers excreted by microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Membrane Fouling Control by HMBR: Effect of Microbial Community on EPS

Liu

Yang

2020

IJERPH

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A hybrid membrane bioreactor (HMBR) employing activated sludge and biofilm simultaneously is proved to represent a good performance on membrane fouling control compared to conventional membrane bioreactor (CMBR) by reducing extracellular polymeric substances (EPS), especially bound EPS (B-EPS). In order to better understand the mechanism of membrane fouling control by the HMBR in regard of microbial community composition, a pilot scale HMBR operated to treat domestic wastewater for six months, and a CMBR operated at the same time as control group. Results showed that HMBR can effectively control membrane fouling. When transmembrane pressure reached 0.1 MPa, the membrane module in the HMBR operated for about 26.7% longer than that in the CMBR. In the HMBR, the quantity of EPS was significantly lower than that in the CMBR. In this paper, soluble EPS was also found to have a close relationship with cake layer resistance. The species richness and diversity in the HMBR were higher than those in the CMBR, and a certain difference between the compositions of microbial communities in the two reactors was confirmed. Therefore, the difference in microbial community compositions may be the direct reason why EPS in the HMBR was lower than that in the CMBR.

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Section: Introductionmentioning

confidence: 99%

Mechanism of Membrane Fouling Control by HMBR: Effect of Microbial Community on EPS

Liu

Yang

2020

IJERPH

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“…The principle of HPSEC based on difference in retention times/intensity of the molecules which is characterized by size and concentration. Small molecules can penetrate the column of HPSEC chromatography quickly, while very large molecules penetrate the column slowly [24].…”

Section: Methodsmentioning

confidence: 99%

Ozonation and Depolymerization of Extracellular Polymeric Substances (EPS) Extracted from a Biofilter Treating Gaseous Toluene

Baig

Saingam

et al. 2018

Polymers

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Low-concentration ozonation was developed as a novel technique to control the excess biomass in volatile organic compound (VOC) biofilters. In order to understand the reaction mechanism between ozone and biomass, the changes in properties of ozone exposed extracellular polymeric substances (EPS) were investigated in this study. EPS was sequestered from the biofilm, obtained from a biofilter treating gaseous toluene, and then it was exposed to gaseous ozone at 272 ± 22 ppm continuously for 12 h. The total organic carbon (TOC) results indicated that low concentration ozone could not mineralize the EPS to carbon dioxide (CO2) completely. The excitation-emission matrix fluorescence spectroscopy (EEM) results demonstrated that ozone preferred to attack the benzene ring and specific amino acid residues (such as tryptophan) on the protein chain. High performance size-exclusion chromatography (HPSEC) results confirmed that the protein molecules were depolymerized after ozone attack, while the molecular weight of polysaccharides was not much affected by ozone. During ozonation, few volatile organic compounds (VOCs), such as carboxylic acids, aldehydes, ketones, benzaldehyde and by-products of toluene, were generated, which confirms a minor change in the TOC concentration of EPS. Results revealed that low concentration ozone can reduce the molecular weight of biofilter EPS which can be a key reason for controlling biomass accumulation. Additionally, this can be used to study the composition of biofilm EPS from biofilters.

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“…The soluble and bound EPS were calculated by summing proteins, carbohydrates and humic acids, applying a method optimized by our group (Domínguez Chabaliná et al 2013). EPS extraction was carried out by ion exchange using a cation resin (Frolund et al 1996).…”

Section: Carbamazepinamentioning

confidence: 99%

Use of combined UASB + eMBR treatment for removal of emerging micropollutants and reduction of fouling

Mora-Cabrera

Peña-Guzmán

Trapote

et al. 2021

Journal of Water Supply: Research and Technology-Aqua

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This study employs a novel combined pilot plant consisting of an anaerobic reactor followed by a membrane electro-bioreactor (eMBR) to treat domestic water containing selected micro-pollutants of emerging concern (CECs) [ibuprofen (IB), carbamazepine, diclofenac (DCF) and 17α-ethinylestradiol (EE2)]. The first phase operated as a conventional membrane bioreactor to achieve the removal of organic matter [chemical oxygen demand (COD)], the CECs and phosphorus. A removal rate of 96.3% for COD, 94.5% for IB, 37.1% for CMZ, 87.1% for DCF and 96% for EE2 was obtained. In the three subsequent phases, current density (CD) of 5, 10 and 15 A/m2 was applied successively in the eMBR with the aim of investigating the effects on the removal of the former components and the fouling of the membrane. After the application of 5 and 10 A/m2 CD, the removal rate of COD decreased. Regarding phosphorus, a CD of 5 A/m2 was enough to achieve the rate of 97% and the membrane fouling suffered a substantial reduction too. Finally, the experimental results were subject to statistical analysis using the Kruskal–Wallis and Wilcoxon tests to validate the influence of each DC.

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Characterization of soluble and bound EPS obtained from 2 submerged membrane bioreactors by 3D-EEM and HPSEC

Cited by 50 publications

References 27 publications

Mechanism of Membrane Fouling Control by HMBR: Effect of Microbial Community on EPS

Mechanism of Membrane Fouling Control by HMBR: Effect of Microbial Community on EPS

Ozonation and Depolymerization of Extracellular Polymeric Substances (EPS) Extracted from a Biofilter Treating Gaseous Toluene

Use of combined UASB + eMBR treatment for removal of emerging micropollutants and reduction of fouling

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