Characterization of soil bacterial community structure and physicochemical properties in created and natural wetlands

Peralta, Rita M.; Ahn, Changwoo; Gillevet, Patrick M.

doi:10.1016/j.scitotenv.2012.11.052

Cited by 142 publications

(62 citation statements)

References 47 publications

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“…Many studies have calculated Shannon diversity index (H ) in aquatic systems and values typically range from 0.5 to 5, with values of 0.5-2.0 implying low diversity and values of 2.0-5.0 indicating high diversity (Garrido et al, 2014). In this study, the majority of H were higher than 2.0, with a range of 1.59-5.60, suggesting a relatively high H value and wide range of diversity for the present treatment wetlands compared to previous studies on CWs (2.0-3.8 in Arroyo et al (2013); 1.74-2.93 in Peralta et al (2013)). …”

Section: Diversity Of Microbial Communitiescontrasting

confidence: 46%

Characterization of bacterial communities in wetland mesocosms receiving pharmaceutical-enriched wastewater

Zhang

Luo

Lee

et al. 2016

Ecological Engineering

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a b s t r a c tA 16S rRNA gene multiplex 454 pyrosequencing approach was used to characterize the structure of the bacterial community in subsurface flow constructed wetlands planted with Typha angustifolia and unplanted wetland mesocosms receiving ibuprofen-enriched wastewater at a concentration of 250 g L −1 . The removal efficiency of ibuprofen (IBP) in the planted beds (78.5%) was significantly higher than that in the unplanted beds (57.9%) (P ≤ 0.05). The presence of ibuprofen significantly (P ≤ 0.05) reduced the diversity of the bacterial community. In total, 20 phyla comprising 265 genera were identified. The most predominant phyla were Firmicutes (27.6%), Actinobacteria (26.0%) and Proteobacteria (24.9%), making up 57-95% of the 16S rDNA sequences. Other dominating phyla in wetland soils were Chloroflexi (7.3%), Bacteroidetes (2.7%), Synergistetes (2.1%), and Acidobacteria (2.0%). A distinct profile of bacterial community in the wetland mesocosms was observed after IBP enrichment. IBP-enrichment enhanced the relative abundance of Actinobacteria, Bacilli and ␥-Proteobacteria at class level. The efficient ibuprofen removal observed in this study suggested that the IBP-enriched wetland systems may have selected a restricted group of bacteria that was able to survive best in the disturbed condition and participate in the IBP biodegradation. Our findings also indicated that the presence of macrophytes may have a vital impact on the microbial community and planted wetlands may be more robust and buffered against significant shifts in microbial composition in response to IBP-disturbance.

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Section: Diversity Of Microbial Communitiescontrasting

confidence: 46%

Characterization of bacterial communities in wetland mesocosms receiving pharmaceutical-enriched wastewater

Zhang

Luo

Lee

et al. 2016

Ecological Engineering

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“…[33] To investigate the microbial community function, we analysed the identified protein sequences from soil and water metagenomic data sets. Hierarchical analysis of SEED Subsystems in MG-RAST revealed that the basic functions of microbes in soil and water were similar (Figure 4).…”

Section: Microbial Functionmentioning

confidence: 99%

Metagenomic analysis reveals microbial diversity and function in the rhizosphere soil of a constructed wetland

Bai

Liang

Liu

et al. 2014

Environmental Technology

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Microbial communities play a critical role in the degradation of effluent contaminants in constructed wetlands. Many questions remain, however, regarding the role of microbial communities in rhizospheric soil. In this study, we used metagenomic analysis to assess microbial community composition and function in a constructed wetland receiving surface water. The diversity of the microbial community of rhizosphere soil was found to be significantly greater than that of the wetland influent water. This enhancement is likely due to the availability of diverse habitats and nutrients provided by the wetland plants. From function annotation of metagenomic data, a number of biodegradation pathways associated with 14 xenobiotic compounds were identified in soil. Nitrogen fixation, nitrification and denitrification genes were semi-quantitatively analysed. By screening of manganese transformation genes, we found that the biological oxidation of Mn 2+ (mainly catalysed by multicopper oxidase) in the influent water yielded insoluble Mn 4+ , which subsequently precipitated and were incorporated into the wetland soil. These data show that the use of metagenomic analysis can provide important new insights for the study of wetland ecosystems and, in particular, how biologically mediated transformation or degradation can be used to reduce contamination of point and non-point source wastewater.

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“…However, these methods may overlook many of the low-abundance organisms in microbial communities, therefore providing incomplete information about the community structure and diversity within CWs [14]. Advances in qualitative and quantitative molecular techniques, such as high-throughput sequencing and quantitative PCR (qPCR), have made it possible to detect the structure and abundance of microbial communities more profoundly [15][16][17]. A number of studies have applied quantitative PCR [18][19][20][21] and high-throughput sequencing [22][23][24][25] to investigate microbial processes in various wastewater treatment systems.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Bacterial Community Abundance and Structure in Horizontal Subsurface Flow Wetland Mesocosms Treating Municipal Wastewater

Oopkaup

Truu

Nõlvak

et al. 2016

Water

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Dynamics of bacterial community abundance and structure of a newly established horizontal subsurface flow (HSSF) pilot-scale wetland were studied using high-throughput sequencing and quantitative polymerase chain reaction (PCR) methods. Bacterial community abundance increased rapidly within one month and stabilised thereafter in three replicate HSSF constructed wetland (CW) mesocosms. The most dominant phylum was Proteobacteria, followed by Bacteroidetes in wetland media biofilms and Firmicutes in influent wastewater. CW bacterial community diversity increased over time and was positively related to the wastewater treatment efficiency. Increase in the abundance of total bacteria in the community was accompanied with the abundance of denitrifying bacteria that promoted nitrate and nitrite removal from the wastewater. During the 150-day study period, similar patterns of bacterial community successions were observed in replicate HSSF CW mesocosms. The data indicate that successions in the bacterial community in HSSF CW are shaped by biotic interactions, with a significant contribution made by external abiotic factors such as influent chemical parameters. Network analysis of the bacterial community revealed that organic matter and nitrogen removal in HSSF CW could be, in large part, allocated to a small subset of tightly interconnected bacterial species. The diversity of bacterial community and abundance of denitrifiers were good predictors of the removal efficiency of ammonia, nitrate and total organic C in HSSF CW mesocosms, while the removal of the seven-day biochemical oxygen demand (BOD 7 ) was best predicted by the abundance of a small set of bacterial phylotypes. The results suggest that nitrogen removal in HSSF CW consist of two main pathways. The first is heterotrophic nitrification, which is coupled with aerobic denitrification and mediated by mixotrophic nitrite-oxidizers. The second pathway is anaerobic denitrification, which leads to gaseous intermediates and loss of nitrogen as N 2 .

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Characterization of soil bacterial community structure and physicochemical properties in created and natural wetlands

Cited by 142 publications

References 47 publications

Characterization of bacterial communities in wetland mesocosms receiving pharmaceutical-enriched wastewater

Characterization of bacterial communities in wetland mesocosms receiving pharmaceutical-enriched wastewater

Metagenomic analysis reveals microbial diversity and function in the rhizosphere soil of a constructed wetland

Dynamics of Bacterial Community Abundance and Structure in Horizontal Subsurface Flow Wetland Mesocosms Treating Municipal Wastewater

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