Characterization of sodium hyaluronate blends using frit inlet asymmetrical flow field-flow fractionation and multiangle light scattering

Ali, Muhammad; Hwang, Euijin; Cho, Il Hwan; Moon, Myeong Hee

doi:10.1007/s00216-011-5531-0

Cited by 10 publications

(2 citation statements)

References 32 publications

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“…They found significantly lower extraneural and intraneural fibrosis, and lower adhesion to the surrounding tissue in the experimental group as an explanation to better nerve regeneration. In 2011, Park et al used HA-CMC solution (Guardix ® -sol, 2580 kDa [31]) in a rat sciatic nerve transection model because they thought that the solution-form hyaluronan blend would be more suitable for the weak peripheral nerve repair site than the solid-film hyaluronan blend [23]. Similar results including less perineural scar formation, less fibroblasts, less inflammatory cells and less adhesion with better nerve regeneration were observed.…”

Section: Discussionmentioning

confidence: 98%

The effect of molecular weight and concentration of hyaluronan on the recovery of the rat sciatic nerve sustaining acute traumatic injury

Lan¹,

Yang²,

Lee³

et al. 2017

Biomed. Mater.

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Acute traumatic peripheral nerve injury remains a significant clinical issue affecting mostly young individuals and their productivity in spite of advances in current medicine. Hyaluronan has been explored in this scenario for its anti-adhesive and high biocompatibility properties for decades. The molecular weight and concentration of the locally applied hyaluronan has been overlooked and not optimized. We used different molecular weights and concentrations of hyaluronan in a rat sciatic nerve crush injury model and found better overall outcomes with high molecular weight (3000 kDa) hyaluronan. The anti-inflammatory effect of the higher molecular weight hyaluronan may have a more favorable effect. We conclude that the optimization of hyaluronan is necessary when incorporating hyaluronan in the engineering of biomaterials for use in acute traumatic peripheral nerve injury.

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Section: Discussionmentioning

confidence: 98%

The effect of molecular weight and concentration of hyaluronan on the recovery of the rat sciatic nerve sustaining acute traumatic injury

Lan¹,

Yang²,

Lee³

et al. 2017

Biomed. Mater.

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“…With the application of a hydrodynamic relaxation technique using a frit inlet in asymmetrical flow FFF, the focusing process can be completely avoided. This technique has shown its efficiency for fractionation of different macromolecules and particles like lipoprotein particles [40], carbon nanotubes [41], sodium hyaluronate [42][43][44] and ultrahigh-molecular weight cationic polyacrylamide [45] reducing risk of adsorption on the FFF membrane and/or sample aggregation.…”

Section: Introductionmentioning

confidence: 99%

Frit inlet field-flow fractionation techniques for the characterization of polyion complex self-assemblies

Till

Gaucher

Amouroux

et al. 2017

Journal of Chromatography A

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Polymer self-assemblies joining oppositely charged chains, known as polyion complexes (PICs), have been formed using poly(ethyleneoxide - b - acrylic acid)/poly(l-lysine), poly(ethyleneoxide-b-acrylic acid)/dendrigraft poly(l-lysine) and poly[(3-acrylamidopropyl) trimethylammonium chloride - b - N - isopropyl acrylamide]/poly(acrylic acid). The self-assemblies have been first characterized in batch by Dynamic Light Scattering. In a second step, their analysis by Flow Field-Flow Fractionation techniques (FlFFF) was examined. They were shown to be very sensitive to shearing, especially during the focus step of the fractionation, and this led to an incompatibility with asymmetrical FlFFF. On the other hand, Frit Inlet FlFFF proved to be very efficient to observe them, either in its symmetrical (FI-FlFFF) or asymmetrical version (FI-AsFlFFF). Conditions of elution were found to optimize the sample recovery in pure water. Spherical self-assemblies were detected, with a size range between 70-400nm depending on the polymers. Compared to batch DLS, FI-AsFlFFF clearly showed the presence of several populations in some cases. The influence of salt on poly(ethyleneoxide-b-acrylic acid) (PEO-PAA) 6000-3000/dendrigraft poly(l-lysine) (DGL 3) was also assessed in parallel in batch DLS and FI-AsFlFFF. Batch DLS revealed a first process of swelling of the self-assembly for low concentrations up to 0.8M followed by the dissociation. FI-AsFlFFF furthermore indicated a possible ejection of DGL3 from the PIC assembly for concentrations as low as 0.2M, which could not be observed in batch DLS.

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Insight into hyaluronic acid molecular weight control

Marcellin

Steen

Nielsen

2014

Appl Microbiol Biotechnol

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Hyaluronic acid (HA) is a ubiquitous polysaccharide found in humans, animals, bacteria, algae and molluscs. Simple yet sophisticated, HA demonstrates unique and valuable rheological properties. In solution, HA behaves as a stiffened random coil and the resultant behaviour, even at low concentrations, is far from Newtonian or 'ideal'. These rheological properties are heavily influenced by molecular weight (MW), so it is not surprising that many of the biological functions of HA are dependent on molecular size. The current billion dollar market for HA continues to grow rapidly, both in gross production and the number of applications for its use. Increasing demand, in conjunction with a reticence to use animal-derived HA, has revitalised the market for HA produced by bacterial fermentation. Although the genes and pathways involved in bacterial production of HA are well characterised, the mechanisms that underlie HA MW control are less well understood. By performing a thorough analysis of the proposed mechanisms of MW control in bacterial fermentation, this mini-review tries to elucidate the challenges and future directions for bacterial HA biosynthesis.

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Characterization of sodium hyaluronate blends using frit inlet asymmetrical flow field-flow fractionation and multiangle light scattering

Cited by 10 publications

References 32 publications

The effect of molecular weight and concentration of hyaluronan on the recovery of the rat sciatic nerve sustaining acute traumatic injury

The effect of molecular weight and concentration of hyaluronan on the recovery of the rat sciatic nerve sustaining acute traumatic injury

Frit inlet field-flow fractionation techniques for the characterization of polyion complex self-assemblies

Insight into hyaluronic acid molecular weight control

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