Characterization of SMV resistance of soybean produced by genetic transformation of SMV-CP gene in RNAi

Kim, Hye Jeong; Kim, Mi Jin; Pak, Jung Hun; Jung, Ho Won; Choi, Hong Kyu; Lee, Yeonghoon; Ko, Jong-Min; Jeong, Soon‐Chun; Pack, In Sook; Ryu, Ki Hyun; Chung, Youngjin

doi:10.1007/s11816-013-0279-y

Cited by 36 publications

(29 citation statements)

References 25 publications

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“…We have proven the efficiency of transformation with dipping with high concentration of Agrobacterium solution, sonication and vacuum during the inoculation procedure (Kim et al 2012;Kim et al 2013;Kim et al 2016). Our method also included the addition of thiol compound including sodium thiosulfate, L-cysteine and dithiothreitol (DTT) in co-cultivation stage to prevent the cell damage.…”

Section: Discussionmentioning

confidence: 99%

“…Soybean transgenic plants with multiple functions have been developed using Agrobacterium-mediated transformation system based on the cotyledonary-node (CN) method (Hinchee et al 1988) and improved by using half-seed explants (Paz et al 2006). The trials with additional treatments including the mixture of thiol compounds, sonication and vacuum have elevated transformation efficiency to much higher level (Meurer et al 1998;Olhoft et al 2003;Kim et al 2012;Kim et al 2013;Kim et al 2016). With the high transformation rate, various genes could be introduced into soybean to investigate any physiological change or to achieve agronomical improvement.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Kim¹,

Kim²,

Pak³

et al. 2017

Plant Breed. Biotech

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Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing AtSZF2 were produced to investigate that its ectopic overexpression enhanced salt stress tolerance by Agrobacterium-mediated transformation using half-seed explants. Sixteen transgenic lines were chosen to analyze for T-DNA insertion and transcription levels, and most of them were confirmed as positive. In further analysis with Southern blot, stable transformation event and copy number were confirmed. Following high salinity stress on the detached leaf and whole plant of two transgenic lines (#4 and #6) revealed that the ectopic expression of AtSZF2 was correlated with stress tolerance in phenotype, ion leakage and chlorophyll content with statistical significance. In another test with 20% PEG treatment, similar tolerance of transgenic plants was observed with lower ion leakage and higher chlorophyll content, indicating that the damage of cell membrane was prevented in transgenic plants. Finally, expression of various abiotic stress-responding genes was detected by reverse transcriptase and quantitative real-time PCR analysis with the transgenic plants. It could be proposed that introduction of AtSZF2 resulted in the modulation of ABA/stress responsive gene expression in transgenic soybean plants and make them tolerant against salt stress. Considering soybean as a salt-sensitive crop and importance of salt stress tolerance in specific farming region, the introduction of AtSZF2 may provide an approach for crop improvement in soybean breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Kim¹,

Kim²,

Pak³

et al. 2017

Plant Breed. Biotech

Self Cite

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“…The desired destination vector, pCSEN (provided by Dr. D.H. Lee) was used for the vector construction. The plasmid, pCSEN-ATPG7 was constructed and transformed into Agrobacterium tumefaciens strain EHA105 (Karimi et al 2002), following the protocol described by Kim et al (2013Kim et al ( , 2016Kim et al ( , 2017.…”

Section: Methodsmentioning

confidence: 99%

“…Kwangankong) were utilized in Agrobacterium-mediated transformation experiments, following the protocol described by Kim et al (2013Kim et al ( , 2016Kim et al ( , 2017. T0 plants with two trifoliates were screened using an herbicide assay to determine the resistance and to identify putative transformants that express Bar gene.…”

Section: Soybean Transformationmentioning

confidence: 99%

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Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean

Kim

Cho

Pak

et al. 2017

Plant Breed. Biotech.

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AT-hook proteins of plant have shown to be involved in growth and development through the modification of chromatin architecture to co-regulate transcription of genes. Recently, many genes encoding AT-hook protein have been identified and their involvement in senescence delay is investigated. In this study, soybean transgenic plants overexpressing chromatin architecturecontrolling ATPG7 gene was produced by Agrobacterium-mediated transformation and investigated for the positive effect on the important agronomic traits mainly focusing on yield-related components. A total of 27 transgenic soybean plants were produced from about 400 explants. T1 seeds were harvested from all transgenic plants. In the analysis of genomic DNAs from soybean transformants, ATPG7 and Bar fragments were amplified as expected, 975 bp and 408 bp in size, respectively. And also exact gene expression was confirmed by reverse transcriptase-PCR (RT-PCR) from transgenic line #6, #7 and #8. In a field evaluation of yield components of ATPG7 transgenic plants (T3), higher plant height, more of pod number and greater average total seed weight were observed with statistical significance. The results of this study indicate that the introduction of ATPG7 gene in soybean may have the positive effect on yield components.

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GM Crops for Plant Virus Resistance: A Review

Johnson

Gopal

Sudhakar

2020

Genetically Modified Crops

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Characterization of SMV resistance of soybean produced by genetic transformation of SMV-CP gene in RNAi

Cited by 36 publications

References 25 publications

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean

GM Crops for Plant Virus Resistance: A Review

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